Detection of Cryptosporidium molnari oocysts from fish by fluorescent-antibody staining assays for Cryptosporidium spp. affecting humans

Applied and Environmental Microbiology

Volumn 77, Issue 5, 2011, Pages 1878-1880

  Barugahare, Rona ^a   Dennis, Michelle M ^a   Becker, Joy A ^a   Šlapeta, Jan ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

CRYPTOSPORIDIUM; CRYPTOSPORIDIUM SPP; FORMALIN; OOCYSTS; RIBOSOMAL DNA (RDNA); STAINING ASSAYS;

ANTIBODIES; ASSAYS; FLUORESCENCE; FORMALDEHYDE;

CHEMICAL DETECTION;

DIAGNOSTIC AGENT; PROTOZOAL DNA; PROTOZOAL RNA; PROTOZOON ANTIBODY; RIBOSOME DNA; RNA 18S;

ANTIBODY; BIOASSAY; EXPERIMENTAL STUDY; PERCIFORM; PROTOZOAN;

ANIMAL; ANIMAL DISEASE; ARTICLE; CHEMISTRY; CLASSIFICATION; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM; DNA SEQUENCE; FISH DISEASE; FLUORESCENT ANTIBODY TECHNIQUE; GADIFORMES; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; OOCYST; PARASITOLOGY; RNA GENE; STAINING;

ANIMALS; ANTIBODIES, PROTOZOAN; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM; DNA, PROTOZOAN; DNA, RIBOSOMAL; FISH DISEASES; FLUORESCENT ANTIBODY TECHNIQUE, DIRECT; GADIFORMES; GENES, RRNA; MOLECULAR SEQUENCE DATA; OOCYSTS; PARASITOLOGY; RNA, PROTOZOAN; RNA, RIBOSOMAL, 18S; SEQUENCE ANALYSIS, DNA; STAINING AND LABELING;

CRYPTOSPORIDIUM; CRYPTOSPORIDIUM MOLNARI; MACCULLOCHELLA PEELII PEELII;

EID: 79953216392     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02691-10     Document Type: Article

References (23)

1. Alvarez-Pellitero, P., and A. Sitjà-Bobadilla. 2002. Cryptosporidium molnari n. sp. (Apicomplexa: Cryptosporidiidae) infecting two marine fish species, Sparus aurata L. and Dicentrarchus labrax L. Int. J. Parasitol. 32:1007-1021.
2. Arnold, M. M., et al. 1996. Effects of fixation and tissue processing on immunohistochemical demonstration of specific antigens. Biotech. Histochem. 71:224-230.
3. Battifora, H., and M. Kopinski. 1986. The influence of protease digestion and duration of fixation on the immunostaining of keratins. A comparison of formalin and ethanol fixation. J. Histochem. Cytochem. 34:1095-1100.
4. Bonnin, A., J. F. Dubremetz, and P. Camerlynck. 1991. Characterization and immunolocalization of an oocyst wall antigen of Cryptosporidium parvum (Protozoa, Apicomplexa). Parasitology 103:171-177.
5. Chalmers, R. M., and A. P. Davies. 2010. Minireview: clinical cryptosporidiosis. Exp. Parasitol. 124:138-146.
6. Entrala, E., Y. Sbihi, M. Sanchez-Moreno, and C. Mascaro. 2001. Antigen incorporation on Cryptosporidium parvum oocyst walls. Mem. Inst. Oswaldo Cruz 96:233-235.
7. Fayer, R. (ed.). 1997. Cryptosporidium and cryptosporidiosis. CRC Press, Boca Raton, FL.
8. Fayer, R. 2010. Taxonomy and species delimitation in Cryptosporidium. Exp. Parasitol. 124:90-97.
9. Gabor, L., et al. Cryptosporidiosis in intensively reared Barramundi (Lates calcarifer). J. Vet. Diagn. Invest., in press.
10. Ganzenmueller, T., et al. 2010. Comparison of the performance of direct fluorescent antibody staining, a point-of-care rapid antigen test and virus isolation with that of RT-PCR for the detection of novel 2009 influenza A (H1N1) virus in respiratory specimens. J. Med. Microbiol. 59:713-717.
11. Garcia, L. S., A. C. Shum, and D. A. Bruckner. 1992. Evaluation of a new monoclonal-antibody combination reagent for direct fluorescence detection of Giardia and Cryptosporidium oocysts in human fecal specimens. J. Clin. Microbiol. 30:3255-3257.
12. Graczyk, T. K., M. R. Cranfield, and R. Fayer. 1995. A comparative assessment of direct fluorescence antibody, modified acid-fast stain, and sucrose flotation techniques for detection of Cryptosporidium serpentis oocysts in snake fecal specimens. J. Zoo Wildl. Med. 26:396-402.
13. Green, S. L., D. M. Bouley, C. A. Josling, and R. Fayer. 2003. Cryptosporidiosis associated with emaciation and proliferative gastritis in a laboratoryreared South African clawed frog (Xenopus laevis). Comp. Med. 53:81-84.
14. Murphy, B. G., D. Bradway, T. Walsh, G. E. Sanders, and K. Snekvik. 2009. Gastric cryptosporidiosis in freshwater angelfish (Pterophyllum scalare). J. Vet. Diagn. Invest. 21:722-727.
15. Palenzuela, O., P. Alvarez-Pellitero, and A. Sitjà-Bobadilla. 2010. Molecular characterization of Cryptosporidium molnari reveals a distinct piscine clade. Appl. Environ. Microbiol. 76:7646-7649.
16. Rimhanen-Finne, R., H. L. Enemark, J. Kolehmainen, P. Toropainen, and M. L. Hanninen. 2007. Evaluation of immunofluorescence microscopy and enzyme-linked immunosorbent assay in detection of Cryptosporidium and Giardia infections in asymptomatic dogs. Vet. Parasitol. 145: 345-348.
17. Ryan, U., et al. 2003. Identification of novel Cryptosporidium genotypes from the Czech Republic. Appl. Environ. Microbiol. 69:4302-4307.
18. Sitjà-Bobadilla, A., F. Padros, C. Aguilera, and P. Alvarez-Pellitero. 2005. Epidemiology of Cryptosporidium molnari in Spanish gilthead sea bream (Sparus aurata L.) and European sea bass (Dicentrarchus labrax L.) cultures: from hatchery to market size. Appl. Environ. Microbiol. 71:131-139.
19. Šlapeta, J. 2009. Centenary of the genus Cryptosporidium: from morphological to molecular species identification, p. 31-50. In M. G. Ortega-Pierres et al. (ed.), Giardia and Cryptosporidium: from molecules to disease. CAB International, Wallingford, United Kingdom.
20. Šlapeta, J. 2006. Cryptosporidium species found in cattle: a proposal for a new species. Trends Parasitol. 22:469-474.
21. Xiao, L., R. Fayer, U. Ryan, and S. J. Upton. 2004. Cryptosporidium taxonomy: recent advances and implications for public health. Clin. Microbiol. Rev. 17:72-97.
22. Yu, J. R., S. P. O'Hara, J. L. C. Lin, M. E. Dailey, and G. Cain. 2002. A common oocyst surface antigen of Cryptosporidium recognized by monoclonal antibodies. Parasitol. Res. 88:412-420.
23. Zanguee, N., et al. 2010. Identification of novel Cryptosporidium species in aquarium fish. Vet. Parasitol. 174:43-48.