Contribution of the Klebsiella pneumoniae capsule to bacterial aggregate and biofilm microstructures

Applied and Environmental Microbiology

Volumn 77, Issue 5, 2011, Pages 1777-1782

  Dzul, Stephen P ^a   Thornton, Margaret M ^b   Hohne, Danial N ^a   Stewart, Elizabeth J ^a   Shah, Aayush A ^a   Bortz, David M ^c   Solomon, Michael J ^a   Younger, John G ^b  

^a UNIVERSITY OF MICHIGAN   (United States)

^b UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^c UNIVERSITY OF COLORADO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AGGREGATE SIZE DISTRIBUTIONS; AGGREGATE STRUCTURES; BACTERIAL AGGREGATES; FLUID DYNAMIC FORCES; GROWTH CONDITIONS; HIGH RESOLUTION; HIGH-SPEED; HYDRODYNAMIC CONDITIONS; IMAGE-PROCESSING ALGORITHMS; KLEBSIELLA PNEUMONIAE; LAMINAR-TURBULENT TRANSITION; LENGTH SCALE; MACROSCOPIC STRUCTURE; MULTICELLULAR STRUCTURE; REYNOLDS; WILD TYPES;

AGGREGATES; ALIGNMENT; BACTERIOLOGY; BIOFILMS; BIOFILTERS; CONFOCAL MICROSCOPY; FEATURE EXTRACTION; FLUID DYNAMICS; GROWTH (MATERIALS); HYDRODYNAMICS; SIZE DISTRIBUTION;

AGGLOMERATION;

AGGREGATE SIZE; ALGORITHM; BACTERIUM; BIOFILM; GROWTH RATE; HYDRODYNAMICS; IMAGE PROCESSING; MICROSCOPY; MICROSTRUCTURE; MUTATION; REYNOLDS NUMBER; SIZE DISTRIBUTION;

ARTICLE; BACTERIAL MEMBRANE; BACTERIUM ADHERENCE; BIOFILM; CONFOCAL MICROSCOPY; GROWTH, DEVELOPMENT AND AGING; KLEBSIELLA PNEUMONIAE; METABOLISM; METHODOLOGY;

BACTERIAL ADHESION; BACTERIAL CAPSULES; BIOFILMS; KLEBSIELLA PNEUMONIAE; MICROSCOPY, CONFOCAL;

BACTERIA (MICROORGANISMS); KLEBSIELLA PNEUMONIAE;

EID: 79953208650     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01752-10     Document Type: Article

References (18)

1. Allen, M., G. Evans, D. Frenkel, and B. Mulder. 1993. Hard convex body fluids. Adv. Chem. Physics 86:1-166.
2. Bortz, D., T. Jackson, K. Taylor, A. Thompson, and J. Younger. 2008. Flocculation dynamics of Klebsiella pneumoniae. Bull. Math. Biol. 70:745- 768.
3. Bratby, J. 2006. Coagulation and flocculation in water and wastewater treatment, 2nd ed. IWA Publishing, London, United Kingdom.
4. Buchs, J., U. Maier, C. Milbradt, and B. Zoels. 2000. Power consumption in shaking flasks on rotary shaking machines: II. Nondimensional description of specific power consumption and flow regimes in unbaffled flasks at elevated liquid viscosity. Biotechnol. Bioeng. 68:594-601.
5. Crocker, J., and D. Grier. 1996. Methods of digital microscopy for colloidal studies. J. Colloid Interface Sci. 179:298-310.
6. Dutton, G., and A. Lim. 1985. Structural investigation of the capsular polysaccharide of Klebsiella serotype K35. Carbohydr. Res. 145:67-80.
7. Faruque, S., et al. 2006. Transmissibility of cholera: in vivo-formed biofilms and their relationship to infectivity and persistence in the environment. Proc. Nat. Acad. Sci. U. S. A. 103:6350-6355.
8. Favre-Bonte, S., B. Joly, and C. Forestier. 1999. Consequences of reduction of Klebsiella pneumoniae capsule expression on interactions of this bacterium with epithelial cells. Infect. Immun. 67:554-561.
9. Gregory, J. 2006. Particles in water: properties and processes. IWA Publishing, London, United Kingdom.
10. Heydorn, A., et al. 2000. Quantification of biofilm structures by the novel computer program COMSTAT. Microbiology 146:2395-2407.
11. Hohne, D. 2008. Characterization of industrial and biological complex fluids using confocal microscopy. Ph.D. dissertation. University of Michigan, Ann Arbor, MI.
12. Hohne, D., J. Younger, and M. Solomon. 2009. Flexible microfluidic device for mechanical property characterization of soft viscoelastic solids such as bacterial biofilms. Langmuir 25:7743-7751.
13. Mohraz, A., and M. Solomon. 2005. Direct visualization of colloidal rod assembly by confocal microscopy. Langmuir 21:5298-5306.
14. Podschun, R., and U. Ullman. 1998. Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clin. Microbiol. Rev. 11:589-603.
15. R Development Core Team. 2008. R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria.
16. Schembri, M., J. Blom, K. Krogfelt, and P. Klemm. 2005. Capsule and fimbria interaction in Klebsiella pneumoniae. Infect. Immun. 73:4626-4633.
17. Stoodley, P., et al. 2001. Growth and detachment of cell clusters from mature mixed species biofilms. Appl. Environ. Microbiol. 67:5608-5613.
18. Verstrepen, K., and F. Klis. 2006. Flocculation, adhesion, and biofiolm formation in yeast. Mol. Microbiol. 60:5-15.