Three hours of perfusion culture prior to 28 days of static culture, enhances osteogenesis by human cells in a collagen GAG scaffold

Biotechnology and Bioengineering

Volumn 108, Issue 5, 2011, Pages 1203-1210

  Keogh, Michael B ^a   Partap, Sonia ^a,b   Daly, Jacqueline S ^a   O'Brien, Fergal J ^a,b  

^a ROYAL COLLEGE OF SURGEONS IN IRELAND   (Ireland)

^b TRINITY COLLEGE DUBLIN   (Ireland)

Author keywords

Collagen; Flow perfusion bioreactor; Osteogenesis; Scaffold; Tissue engineering

Indexed keywords

BONE TISSUE ENGINEERING; CELL DISTRIBUTION; CELL NUMBER; COMPRESSIVE MODULI; CONTROL GROUPS; EXTRACELLULAR MATRICES; FLOW PERFUSION BIOREACTOR; GENE EXPRESSION ANALYSIS; GLYCOSAMINOGLYCANS; HUMAN CELLS; HUMAN OSTEOBLAST CELLS; IN-VITRO; IN-VIVO; METABOLIC ACTIVITY; MINERAL DEPOSITIONS; OSTEOGENESIS; OSTEOGENIC; OSTEOGENIC PHENOTYPE; PERFUSION CULTURES; REGULATORY SIGNALS; SHORT TERM; STATIC CONTROL; STATIC CULTURE;

ANIMAL CELL CULTURE; BIOCONVERSION; BIOREACTORS; CELL CULTURE; COLLAGEN; GENE EXPRESSION; MECHANICAL TESTING; OSTEOBLASTS; TISSUE;

SCAFFOLDS (BIOLOGY);

ALKALINE PHOSPHATASE; BIOLOGICAL MARKER; CELL DNA; CHONDROITIN 6 SULFATE; COLLAGEN TYPE 1; GENOMIC DNA; GLYCOSAMINOGLYCAN; MESSENGER RNA; MINERAL; OSTEOCALCIN; OSTEONECTIN; OSTEOPONTIN; TISSUE SCAFFOLD;

ARTICLE; BIOREACTOR; BIOREACTOR CULTURE; BONE DEVELOPMENT; CELL COUNT; CELL CULTURE; CELL ENCAPSULATION; CELL METABOLISM; CELL VIABILITY; CELLULAR DISTRIBUTION; CONTROLLED STUDY; EQUIPMENT DESIGN; FLOW PERFUSION BIOREACTOR; GENE EXPRESSION; HISTOLOGY; HUMAN; HUMAN CELL; MINERALIZATION; OSTEOBLAST; RIGIDITY; STATIC CULTURE; TISSUE ENGINEERING;

CELLS, CULTURED; COLLAGEN; GLYCOSAMINOGLYCANS; HUMANS; OSTEOGENESIS; PERFUSION;

EID: 79953164380     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.23032     Document Type: Article

References (33)

1. Bancroft GN, Sikavitsas VI, van den Dolder J, Sheffield TL, Ambrose CG, Jansen JA, Mikos AG. 2002. Fluid flow increases mineralized matrix deposition in 3D perfusion culture of marrow stromal osteoblasts in a dose-dependent manner. Proc Natl Acad Sci USA 99(20): 12600-12605.
2. Bjerre L, Bünger CE, Kassem M, Mygind T. 2008. Flow perfusion culture of human mesenchymal stem cells on silicate-substituted tricalcium phosphate scaffolds. Biomaterials 29(17): 2616.
3. Chowdhury TT, Arghandawi S, Brand J, Akanji OO, Bader DL, Salter DM, Lee DA. 2008. Dynamic compression counteracts IL-1beta induced inducible nitric oxide synthase and cyclo-oxygenase-2 expression in chondrocyte/agarose constructs. Arthritis Res Ther 10(2): R35.
4. Compton CC, Butler CE, Yannas IV, Warland G, Orgill DP. 1998. Organized skin structure is regenerated in vivo from collagen-GAG matrices seeded with autologous keratinocytes. J Invest Dermatol 110(6): 908-916.
5. Donahue HJ, Li Z, Zhou Z, Yellowley CE. 2000. Differentiation of human fetal osteoblastic cells and gap junctional intercellular communication. Am J Physiol Cell Physiol 278(2): C315-C322.
6. Elder BD, Athanasiou KA. 2009. Hydrostatic pressure in articular cartilage tissue engineering: From chondrocytes to tissue regeneration. Tissue Eng Part B Rev 15(1): 43-53.
7. Farrell E, O'Brien FJ, Doyle P, Fischer J, Yannas I, Harley BA, O'Connell B, Prendergast PJ, Campbell VA. 2006. A collagen-glycosaminoglycan scaffold supports adult rat mesenchymal stem cell differentiation along osteogenic and chondrogenic routes. Tissue Eng 12(3): 459-468.
8. Frith JE, Thomson B, Genever P. 2010. Dynamic three-dimensional culture methods enhance mesenchymal stem cell properties and increase therapeutic potential. Tissue Eng Part C Methods 16(4): 735-749.
9. Glowacki J, Mizuno S, Greenberger JS. 1998. Perfusion enhances functions of bone marrow stromal cells in three-dimensional culture. Cell Transplant 7(3): 319.
10. Goldstein AS, Juarez TM, Helmke CD, Gustin MC, Mikos AG. 2001. Effect of convection on osteoblastic cell growth and function in biodegradable polymer foam scaffolds. Biomaterials 22(11): 1279.
11. Harris SA, Enger RJ, Riggs BL, Spelsberg TC. 1995. Development and characterization of a conditionally immortalized human fetal osteoblastic cell line. J Bone Miner Res 10(2): 178-186.
12. Haugh MG, Murphy CM, O'Brien FJ. 2010. Novel freeze-drying methods to produce a range of collagen-glycosaminoglycan scaffolds with tailored mean pore sizes. Tissue Eng Part C Methods 16(5): 887-894.
13. Jaasma MJ, O'Brien FJ. 2008. Mechanical stimulation of osteoblasts using steady and dynamic fluid flow. Tissue Eng Part A 14(7): 1213-1223.
14. Jaasma MJ, Plunkett NA, O'Brien FJ. 2008. Design and validation of a dynamic flow perfusion bioreactor for use with compliant tissue engineering scaffolds. J Biotechnol 133(4): 490-496.
15. Janssen FW, Oostra J, Oorschot A, van Blitterswijk CA. 2006. A perfusion bioreactor system capable of producing clinically relevant volumes of tissue-engineered bone: In vivo bone formation showing proof of concept. Biomaterials 27(3): 315-323.
16. Keogh MB, O'Brien FJ, Daly J. 2009. The effects of cross-linking methods on osteogenesis within a collagen GAG scaffold. Bone 44 (Suppl 2): S266.
17. Keogh MB, FJ OB, Daly JS. 2010. A novel collagen scaffold supports human osteogenesis-Applications for bone tissue engineering. Cell Tissue Res 340(1): 169-177.
18. Kim SS, Penkala R, Abrahimi P. 2007. A perfusion bioreactor for intestinal tissue engineering. J Surg Res 142(2): 327.
19. Margolis L, Hatfill S, Chuaqui R, Vocke C, Emmert-Buck M, Linehan WM, Duray PH. 1999. Long term organ culture of human prostate tissue in a NASA-designed rotating wall bioreactor. J Urol 161(1): 290-297.
20. O'Brien FJ, Harley BA, Yannas IV, Gibson L. 2004. Influence of freezing rate on pore structure in freeze-dried collagen-GAG scaffolds. Biomaterials 25(6): 1077-1086.
21. O'Brien FJ, Harley BA, Yannas IV, Gibson LJ. 2005. The effect of pore size on cell adhesion in collagen-GAG scaffolds. Biomaterials 26(4): 433-441.
22. Owan I, Burr DB, Turner CH, Qiu J, Tu Y, Onyia JE, Duncan RL. 1997. Mechanotransduction in bone: Osteoblasts are more responsive to fluid forces than mechanical strain. Am J Physiol 273(3 Pt 1): C810-C815.
23. Partap S, Plunkett NA, Kelly DJ, O'Brien FJ. 2010. Stimulation of osteoblasts using rest periods during bioreactor culture on collagen-glycosaminoglycan scaffolds. J Mater Sci Mater Med 21(8): 2325-2330.
24. Partap S, Lyons F, O'Brien FJ. 2010. Scaffolds & surfaces. Stud Health Technol Inform. Basic Engineering for Medics and Biologist. Amsterdam, The Netherlands: IOS Press, p 187-201.
25. Plunkett NA, Partap S, O'Brien FJ. 2010. Osteoblast response to rest periods during bioreactor culture of collagen-glycosaminoglycan scaffolds. Tissue Eng Part A 16(3): 943-951.
26. Shea LD, Wang D, Franceschi RT, Mooney DJ. 2000. Engineered bone development from a pre-osteoblast cell line on three-dimensional scaffolds. Tissue Eng 6(6): 605-617.
27. Syedain ZH, Tranquillo RT. 2009. Controlled cyclic stretch bioreactor for tissue-engineered heart valves. Biomaterials 30(25): 4078.
28. Tierney CM, Jaasma MJ, O'Brien FJ. 2009. Osteoblast activity on collagen-GAG scaffolds is affected by collagen and GAG concentrations. J Biomed Mater Res A 91(1): 92-101.
29. Wang TW, Wu HC, Wang HY, Lin FH, Sun JS. 2009. Regulation of adult human mesenchymal stem cells into osteogenic and chondrogenic lineages by different bioreactor systems. J Biomed Mater Res A 88(4): 935-946.
30. Wiesmann HP, Joos U, Meyer U. 2004. Biological and biophysical principles in extracorporal bone tissue engineering: Part II. Int J Oral Maxillofac Surg 33(6): 523-530.
31. Yates C, Shepard CR, Papworth G, Dash A, Beer Stolz D, Tannenbaum S, Griffith L, Wells A. 2007. Novel three dimensional organotypic liver bioreactor to directly visualize early events in metastatic progression. Advances in Cancer Research 97:225-246.
32. Yu X, Botchwey EA, Levine EM, Pollack SR, Laurencin CT. 2004. Bioreactor-based bone tissue engineering: The influence of dynamic flow on osteoblast phenotypic expression and matrix mineralization. Proc Natl Acad Sci USA 101(31): 11203-11208.
33. Zhu XH, Arifin DY, Khoo BH, Hua J, Wang C-H. 2009. Study of cell seeding on porous poly(d, l-lactic-co-glycolic acid) sponge and growth in a Couette-Taylor bioreactor. Chem Eng Sci 65(6): 2108.