Assessment of optical clearing agents using reflectance-mode confocal scanning laser microscopy

Journal of Innovative Optical Health Sciences

Volumn 3, Issue 3, 2010, Pages 183-188

  Samatham, Ravikant ^a   Phillips, Kevin G ^a   Jacques, Steven L ^a  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

anisotropy; Optical properties; skin

Indexed keywords

CONFOCAL MICROSCOPES; CONFOCAL SCANNING LASER MICROSCOPY; DEPTH OF FOCUS; GLASS COVERS; MECHANISM OF ACTION; MOUSE SKIN; NUMERICAL APERTURE; OPTICAL CLEARING AGENTS; OPTICAL IMAGING; REFLECTANCE SIGNALS; SCATTERING CO-EFFICIENT; UNTREATED CONTROL;

ANISOTROPY; CONFOCAL MICROSCOPY; GLYCEROL; MAMMALS; OPTICAL PROPERTIES; TISSUE;

REFLECTION;

EID: 79953118967     PISSN: 17935458     EISSN: 17937205     Source Type: Journal    
DOI: 10.1142/S1793545810001064     Document Type: Article

References (9)

1. D. S. Gareau, G. Merlino, C. Corless, M. Kulesz-Martin, S. L. Jacques, "Noninvasive imaging of melanoma with reflectance mode confocal scanning laser microscopy in a murine model", J. Investigat. Dermatol. 27(9), 2184-2190 (2007). (Pubitemid 47258480)
2. R. Samatham, S. L. Jacques, P. Campagnola, "Optical properties of mutant versus wild-type mouse skin measured by reflectance-mode confocal scanning laser microscopy (rCSLM)", J. Biomed. Opt. 13, 041309 (2008).
3. K. G. Phillips, R. Samatham, N. Choudhury, G. C. Gladish, P. Thuillier, S. L. Jacques, "In vivo measurement of epidermal thickness changes associated with tumor promotion in murine models", J. Biomed. Opt. in press (2010).
4. R. K. Wang, X. Xu, V. V. Tuchin, J. B. Elder, "Concurrent enhancement of imaging depth and contrast for optical coherence tomography by hyperosmotic agents", J. Opt. Soc. Am. B 18, 948-953 (2001). (Pubitemid 33298615)
5. S. R. Millon, K. M. Roldan-Perez, K. M. Riching, G. M. Palmer, N. Ramanujam, "Effect of optical clearing agents on the in vivo optical properties of squamous epithelial tissue", Lasers Surg. Med. 38, 920-927 (2006). (Pubitemid 46037473)
6. goyoDMSO is 1.4523 at 546nm at 25°C, from R. G. LeBel, D. A. I. Goring, "Density, viscosity, refractive index, and hygroscopicity of mixtures of water and dimethyl sulfoxide", J. Chem. Eng. Data 7(1), 100-101 (1962).
7. glycerol = 1.4729 at 589.29 nm, from http://en.wikipedia.org/ wiki/List-of-refractiveindices.
8. D. S. Gareau, In Vivo Confocal Microscopy In Turbid Media, Ph. D. Dissertation, Oregon Health and Science University, Portland, Oregon, USA (2006)
9. S. L. Jacques, D. Levitz, R. Samatham, N. Choudhury, F. Truffer, D. S. Gareau, "Light scattering in confocal reflectance microscopy", Chap. 7 in Biomedical Applications of Light Scattering, A. Wax, V. Backman, Eds., McGraw Hill Publishing (2010).