Microfluidic system for single cell sorting with optical tweezers

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 7376, Issue , 2010, Pages

  Bruns, Thomas ^a   Becsi, Laszlo ^b   Talkenberg, Marc ^a   Wagner, Michael ^a   Weber, Petra ^a   Mescheder, Ulrich ^b   Schneckenburger, Herbert ^a  

^a AALEN UNIVERSITY   (Germany)

^b FURTWANGEN UNIVERSITY   (Germany)

Author keywords

Cell sorting; Cell viability; Light dose; Microfluidic system; Optical tweezers

Indexed keywords

CELL SORTING; CELL VIABILITY; CHANNEL STRUCTURES; EXPOSURE TIME; GLASS CHIPS; MICROFLUIDIC SYSTEM; SILICON LAYER; SINGLE CELLS;

BIOASSAY; BIOCHIPS; FLUIDIC DEVICES; MICROFLUIDICS; OPTICAL INSTRUMENTS; POLYMERASE CHAIN REACTION;

OPTICAL TWEEZERS;

EID: 79953116789     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.871450     Document Type: Conference Paper

References (14)

1. Ashkin, A., "Acceleration and Trapping of Particles by Radiation Pressure," Phys. Rev. Lett. 24(4), 156-159 (1969).
2. Neuman, K. C. and Block, S. M., "Optical Trapping," Rev. Sci. Instrum. 75(9), 2787-2809 (2004).
3. Weber, G. and Greulich, K. O. "Manipulation of cells, organelles, and genomes by laser microbeam and optical trap," Int. Rev. Cytol. 133, 1-41 (1992).
4. Liang, H., Wright, W. H., Cheng, S., He, W., and Berns, M. W, "Micromanipulation of chromosomes in PTK2 cells using laser microsurgery (optical scalpel) in combination with laser-induced optical force (optical tweezers), " Exp. Cell Res. 204, 110-120 (1993).
5. Schütze, K. and Clement-Sengewald, A., "Catch and move cut or fuse," Nature (London) 368, 667-669 (1994).
6. Zhang, H. and Liu, K.-K., "Optical tweezers for single cells," J. R. Soc. Interface 5(24), 671-690 (2008).
7. Enger, J., Goksör, M., Ramser, K., Hagberg, P., and Hanstorp, D., "Optical tweezers applied to a microfluidic system," Lab. Chip 4(3), 196-200 (2004).
8. Cran-McGreehin, S., Krauss, T.F., and Dholakia, K., "Integrated monolithic optical manipulation," Lab. Chip 6(9), 1122-1124 (2006).
9. Kovac, J.R. and Voldman, J., "Intuitive, image-based cell sorting using optofluidic cell sorting," Anal. Chem. 79(24), 9321-9330 (2007).
10. Nève, N., Kohles, S.S., Winn, S.R., and Tretheway, D.C., "Manipulation of Suspended Single Cells by Microfluidics and Optical Tweezers," Cell. Mol. Bioeng. 3(3), 213-228 (2010).
11. Murata, M., Okamoto, Y., Park, Y.S., Kaji, N., Tokeshi, M., and Baba, Y., "Cell separation by the combination of microfluidics and optical trapping force on a microchip," Anal. Bioanal. Chem. 394(1), 277-283 (2009).
12. Iliescu, C., Tay, F.E.H., and Miao, J., "Strategies in deep wet etching of Pyrex glass," Sens. Actuators A, 133(2), 395-400 (2007).
13. Wagner, M., Weber, P., Bruns, T., Strauss, W.S.L., Wittig, R, and Schneckenburger, H., "Light dose is a limiting factor to maintain cell viability in fluorescence microscopy and single molecule detection," Int. J. Mol. Sci. 11, 956-966 (2010).
14. Schneckenburger, H., Hendinger, A., Sailer, R., Strauss, W.S.L., and Schmitt, M., "Laser-assisted optoporation of single cells," J. Biomed. Opt. 7, 410-416 (2002).