Identification of Brucella species and biotypes using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)

Critical Reviews in Microbiology

Volumn 31, Issue 4, 2005, Pages 191-196

  Al Dahouk, Sascha ^a   Tomaso, Herbert ^a   Prenger Berninghoff, Ellen ^b   Splettstoesser, Wolf D ^a   Scholz, Holger C ^a   Neubauer, Heinrich ^a  

^a BUNDESWEHR INSTITUTE OF MICROBIOLOGY   (Germany)

^b JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

Brucella; Brucellosis; dnaK; ery; Molecular Typing; omp2; omp25; omp31; PCR RFLP; rpsL

Indexed keywords

BIOTYPE; BRUCELLA; BRUCELLA ABORTUS; BRUCELLA CANIS; BRUCELLA MELITENSIS; BRUCELLA OVIS; BRUCELLA SUIS; BRUCELLOSIS; DNA CLEAVAGE; GENE LOCUS; GENETIC POLYMORPHISM; MOLECULAR EVOLUTION; NONHUMAN; PATHOGENICITY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; REVIEW; ZOONOSIS;

BACTERIAL OUTER MEMBRANE PROTEINS; BRUCELLA; BRUCELLOSIS; GENOME, BACTERIAL; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SPECIES SPECIFICITY;

BRUCELLA; BRUCELLA MELITENSIS; BRUCELLA MELITENSIS BIOVAR ABORTUS; BRUCELLA MELITENSIS BIOVAR CANIS; BRUCELLA MELITENSIS BIOVAR OVIS; BRUCELLA MELITENSIS BIOVAR SUIS;

EID: 28844481481     PISSN: 1040841X     EISSN: None     Source Type: Journal    
DOI: 10.1080/10408410500304041     Document Type: Review

References (25)

1. Al Dahouk, S., Tomaso, H., Nöckler, K., Neubauer, H., and Frangoulidis, D. 2003. Laboratory-based diagnosis of brucellosis - a review of the literature. Part I: Techniques for direct detection and identification of Brucella spp. Clin. Lab. 49, 487-505.
2. Allardet-Servent, A., Bourg, G., Ramuz, M., Pages, M., Bellis, M., and Roizes, G. 1988. DNA polymorphism in strains of the genus Brucella. J. Bacteriol. 170, 4603-1607.
3. Alton, G.G., Jones, L.M., Angus, R.D., and Verger, J.M. 1988. Techniques for the brucellosis laboratory, Paris: Institut National de la Recherche Agronomique.
4. Bardenstein, S., Mandelboim, M., Ficht, T.A., Baum, M., and Banai, M. 2002. Identification of the Brucella melitensis vaccine strain Rev.1 in animals and humans in Israel by PCR analysis of the PstI site polymorphism of its omp2 gene. J. Clin. Microbiol. 40, 1475-1480.
5. Bricker, B.J. 2002. PCR as a diagnostic tool for brucellosis. Vet. Microbiol. 90, 435-446.
6. Bricker, B.J., Ewalt, D.R., and Halling, S.M. 2003. Brucella 'HOOF-Prints': strain typing by multi-locus analysis of variable number tandem repeats (VNTRs). BMC Microbiol. 3, 15, http://www.biomedcentral.com/1471-2180/3/ 15 (accessed 2003 July 11).
7. Clavareau, C., Wellemans, V., Walravens, K., Tryland, M., Verger, J.M., Grayon, M., Cloeckaert, A., Letesson, J.J., and Godfroid, J. 1998. Phenotypic and molecular characterization of a Brucella strain isolated from a minke whale (Balaenoptera acutorostrata). Microbiology 144, 3267-3273.
8. Cloeckaert, A., Grayon, M., and Grépinet, O. 2002. Identification of Brucella melitensis vaccine strain Rev. 1 by PCR-RFLP based on a mutation in the rpsL gene. Vaccine 20, 2546-2550.
9. Cloeckaert, A., Grayon, M., Verger, J.M., Letesson, J.J., and Godfroid, J. 2000. Conservation of seven genes involved in the biosynthesis of the lipopolysaccharide O-side chain in Brucella spp. Res. Microbiol. 151, 209-216.
10. Cloeckaert, A., Verger, J.M., Grayon, M., and Vizcaíno, N. 1996a. Molecular and immunological characterization of the major outer membrane proteins of Brucella. FEMS Microbiol. Lett. 145, 1-8.
11. Cloeckaert, A., Verger, J.M., Grayon, M., and Grépinet, O. 1995. Restriction site polymorphism of the genes encoding the major 25 kDa and 36 kDa outer-membrane proteins of Brucella. Microbiology 141, 2111-2121.
12. Cloeckaert, A., Verger, J.M., Grayon, M., and Grépinet, O. 1996b. Polymorphism at the dnaK locus of Brucella species and identification of a Brucella melitensis species-specific marker. J. Med. Microbiol. 45, 200-205.
13. Cloeckaert, A., Verger, J.M., Grayon, M., Paquet, J.Y., Garin-Bastuji, B., Foster, G., and Godfroid, J. 2001. Classification of Brucella spp. isolated from marine mammals by DNA polymorphism at the omp2 locus. Microbes Infect. 3, 729-738.
14. Corbel, M.J. 1997. Brucellosis: an overview. Emerg. Infect. Dis. 3, 213-221.
15. Da Costa, M., Guillou, J.P., Garin-Bastuji, B., Thiébaud, M., and Dubray, G. 1996. Specificity of six gene sequences for the detection of the genus Brucella by DNA amplification. J. Appl. Bacteriol. 81, 267-275.
16. Ficht, T.A., Bearden, S.W., Sowa, B.A., and Marquis, H. 1990. Genetic variation at the omp2 porin locus of the Brucellae: Species-specific markers. Mol. Microbiol. 4, 1135-1142.
17. Ficht, T.A., Bearden, S.W., Sowa, B.A., and Adams, L.G. 1989. DNA sequence and expression of the 36-kilodalton outer membrane protein gene of Brucella abortus. Infect. Immun. 57, 3281-3291.
18. Ficht, T.A., Husseinen, H.S., Derr, J., and Bearden, S.W. 1996. Species-specific sequences at the omp2 locus of Brucella type strains. Int. J. Syst. Bacteriol. 46, 329-331.
19. García-Yoldi, D., Marín, C.M., and López-Goni, I. 2005. Restriction site polymorphisms in the genes encoding new members of group 3 outer membrane protein family of Brucella spp. FEMS Microbiol. Lett. 245, 79-84.
20. Muzny, D.M., Ficht, T.A., Templeton, J.W., and Adams, L.G. 1989. DNA homology of Brucella abortus strains 19 and 2308. Am. J. Vet. Res. 50, 655-661.
21. O'Hara, M.J., Collins, D.M., and de Lisle, G.W. 1985. Restriction endonuclease analysis of Brucella ovis and other Brucella species. Vet. Microbiol. 10, 425-429.
22. Sangari, F.J., and Agüero, J. 1994. Identification of Brucella abortus B19 vaccine strain by the detection of DNA polymorphism at the ery locus. Vaccine 12, 435-438.
23. Verger, J.M., Grimont, F., Grimont, P.A.D., and Grayon, M. 1985. Brucella, a monospecific genus as shown by deoxyribonucleic acid hybridization. Int. J. Syst. Bacteriol. 35, 292-295.
24. Vizcaíno, N., Cloeckaert, A., Verger, J.M., Grayon, M., and Fernández-Lago, L. 2000. DNA polymorphism in the genus Brucella. Microbes Infect. 2, 1089-1100.
25. Vizcaíno, N., Verger, J.M., Grayon, M., Zygmunt, M.S., and Cloeckaert, A. 1997. DNA polymorphism at the omp-31 locus of Brucella spp.: Evidence for a large deletion in Brucella abortus, and other species-specific markers. Microbiology 143, 2913-2921.