Integrated Raman and angular scattering microscopy reveals chemical and morphological differences between activated and nonactivated CD8+ T lymphocytes

Journal of Biomedical Optics

Volumn 15, Issue 3, 2010, Pages

  Smith, Zachary J ^a   Wang, Jyh Chiang E ^b   Quataert, Sally A ^b   Berger, Andrew J ^a  

^a UNIVERSITY OF ROCHESTER   (United States)

^b UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

Author keywords

Elastic scattering; Multimodal microscopy; Raman spectroscopy

Indexed keywords

ANGULAR SCATTERING; CD8+T-LYMPHOCYTES; CELLULAR PROTEINS; LABEL FREE; MEAN SIZE; MULTI-MODAL; MULTIMODAL MICROSCOPY; OPTICAL TECHNIQUE; PAIRED SAMPLE; PHORBOL MYRISTATE ACETATES; RAMAN BANDS; RESTING STATE; SINGLE CELLS; STAPHYLOCOCCAL ENTEROTOXIN B; T CELLS;

FLOW CYTOMETRY; RAMAN SPECTROSCOPY;

SCATTERING;

STAPHYLOCOCCUS;

ENTEROTOXIN; ENTEROTOXIN B, STAPHYLOCOCCAL; PHORBOL 13 ACETATE 12 MYRISTATE;

ARTICLE; CD8+ T LYMPHOCYTE; DRUG EFFECT; ELASTICITY; FLOW CYTOMETRY; HUMAN; IMMUNOLOGY; LYMPHOCYTE ACTIVATION; METHODOLOGY; MICROSCOPY; RAMAN SPECTROMETRY;

CD8-POSITIVE T-LYMPHOCYTES; ELASTICITY; ENTEROTOXINS; FLOW CYTOMETRY; HUMANS; LYMPHOCYTE ACTIVATION; MICROSCOPY; SPECTRUM ANALYSIS, RAMAN; TETRADECANOYLPHORBOL ACETATE;

EID: 79953075644     PISSN: 10833668     EISSN: 15602281     Source Type: Journal    
DOI: 10.1117/1.3443794     Document Type: Article

References (22)

1. N. Auphan-Anezin, G. Verdeil, and A.-M. Schmitt-Verhulst, "Distinct thresholds for CD8 T cell activation lead to functional heterogeneity: CD8 T cell priming can occur independently of cell division," J. Immunol. 170(5), 2442-2448 (2003). (Pubitemid 36245632)
2. M. R. Branco, T. Branco, F. Ramirez, and A. Pombo, "Changes in chromosome organization during PHA-activation of resting human lymphocytes measured by cryo-FISH," Chromosome Res. 16(3), 413-426 (2008). (Pubitemid 351644861)
3. M. Wölfl, J. Kuball, W. Y. Ho, H. Nguyen, T. J. Manley, M. Bleakley, and P. D. Greenberg, "Activation-induced expression of CD137 permits detection, isolation, and expansion of the full repertoire of CD8+ T cells responding to antigen without requiring knowledge of epitope specificities," Blood 110(1), 201-210 (2007). (Pubitemid 47026836)
4. C. Krafft, T. Knetschke, A. Siegner, R. H. W. Funk, and R. Salzer, "Mapping of single cells by near infrared Raman microspectroscopy," Vib. Spectrosc. 32(5), 75-83 (2003).
5. C. Xie, D. Chen, and Y.-Q. Li, "Raman sorting and identification of single living micro-organisms with optical tweezers," Opt. Lett. 30(14), 1800-1802 (2005). (Pubitemid 41083735)
6. J. W. Chan, D. S. Taylor, T. Zwerdling, S. M. Lane, K. Ihara, and T. Huser, "Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells," Biophys. J. 90(2), 648-656 (2006).
7. M. D. Mannie, T. J. McConnell, C. Xie, and Y.-Q. Li, " Activationdependent phases of T cells distinguished by use of optical tweezers and near infrared Raman spectroscopy," J. Immunol. Methods 297(1), 53-60 (2005). (Pubitemid 40386388)
8. J. W. Chan, D. S. Taylor, S. M. Lane, T. Zwerdling, J. Tuscano, and T. Huser, "Nondestructive identification of individual leukemia cells by laser trapping Raman spectroscopy," Anal. Chem. 80(6), 2180-2187 (2008). (Pubitemid 351458069)
9. J. R. Mourant, A. H. Hielscher, A. A. Eick, T. M. Johnson, and J. P. Freyer, "Evidence of intrinsic differences in the light scattering properties of tumorigenic and nontumorigenic cells," Cancer Cytopathol. 84(6), 366-374 (1998). (Pubitemid 29031398)
10. V. Backman, R. Gurjar, K. Badizadegan, I. Itzkan, R. R. Dasari, L. T. Perelman, and M. S. Feld, "Polarized light scattering spectroscopy for quantitative measurement of epithelial cellular structures in situ," IEEE J. Sel. Top. Quantum Electron. 5(4), 1019-1026 (1999). (Pubitemid 30544900)
11. A. Wax, J. W. Pyhtila, R. N. Graf, R. Nines, C. W. Boone, R. R. Dasari, M. S. Feld, V. E. Steele, and G. D. Stoner, "Prospective grading of neoplastic change in rat esophagus epithelium using angleresolved low-coherence interferometry," J. Biomed. Opt. 10(25), 051604 (2005).
12. J. D. Wilson, C. E. Bigelow, D. J. Calkins, and T. H. Foster, "Light scattering from intact cells reports oxidative-stress-induced mitochondrial swelling," Biophys. J. 88, 2929-2938 (2005). (Pubitemid 40976157)
13. J. D. Wilson, B. R. Giesselman, S. Mitra, and T. H. Foster, "Lysosome-damage-induced scattering changes coincide with release of cytochrome c," Opt. Lett. 32(17), 2517-2519 (2007). (Pubitemid 351302971)
14. N. N. Boustany, S. C. Kuo, and N. V. Thakor, "Optical scatter imaging: subcellular morphometry in situ with Fourier filtering," Opt. Lett. 26(14), 1063-1065 (2001). (Pubitemid 33694749)
15. H. Fang, L. Qiu, E. Vitkin, M. M. Zaman, C. Andersson, S. Salahuddin, L. M. Kimerer, P. B. Cipolloni, M. D. Modell, B. S. Turner, S. E. Keates, I. Bigio, I. Itzkan, S. D. Freedman, R. Bansil, E. B. Hanlon, and L. T. Perelman, "Confocal light absorption and scattering spectroscopic (CLASS) microscopy," Appl. Opt. 46(10), 1760-1769 (2007). (Pubitemid 46659543)
16. Z. J. Smith and A. J. Berger, "Integrated Raman- and angularscattering microscopy," Opt. Lett. 33(7), 714-716 (2008).
17. Z. J. Smith and A. J. Berger, "Validation of an integrated Raman- and angular-scattering microscopy system on heterogeneous bead mixtures and single human immune cells," Appl. Opt. 48(10), D109-D120 (2009).
18. Z. J. Smith and A. J. Berger, "Construction of an integrated Ramanand angular-scattering microscope," Rev. Sci. Instrum. 80, 044302(2009).
19. G. Gouesbet, G. Grehan, and B. Maheu, "Scattering of a Gaussian beam by a Mie scatter center using a Bromwich formalism," J. Opt. (Paris) 16(2), 83-93 (1985). (Pubitemid 15490866)
20. N. Uzunbajakava, A. Lenferink, Y. Kraan, B. Willekens, G. Vrensen, J. Greve, and C. Otto, "Nonresonant Raman imaging of protein distribution in single human cells," Biopolymers 72(1), 1-9 (2003). (Pubitemid 36098152)
21. G. J. Puppels, H. S. P. Garritsen, J. A. Kummer, and J. Greve, "Carotenoids located in human lymphocyte subpopulations and natural killer cells by Raman microspectroscopy," Cytometry A 14(3), 251-256 (1993). (Pubitemid 23089074)
22. J. D. Wilson and T. H. Foster, "Mie theory interpretations of light scattering from intact cells," Opt. Lett. 30(18), 2442-2444 (2005). (Pubitemid 41463514)