The role of C-terminus carbohydrate-binding domain of Vibrio cholerae haemolysin/cytolysin in the conversion of the pre-pore β-barrel oligomer to a functional diffusion channel

Indian Journal of Medical Research

Volumn 133, Issue 2, 2011, Pages 131-137

  Mazumdar, Budhaditya ^a   Ganguly, Sreerupa ^a   Ghosh, Amar N ^a   Banerjee, Kalyan K ^a  

^a NATIONAL INSTITUTE OF CHOLERA AND ENTERIC DISEASES   (India)

Author keywords

Haemolysin; Lectin domain; Lipid bilayer; Pore formation; V. cholerae

Indexed keywords

CARBOHYDRATE BINDING PROTEIN; CYTOLYSIN; HEMOLYSIN; HYDROCARBON; LECTIN; OLIGOMER; PORE FORMING CYTOTOXIC PROTEIN; UNCLASSIFIED DRUG; VIBRIO CHOLERAE CYTOLYSIN HEMOLYSIN; BACTERIAL PROTEIN; HEMOLYSIN, VIBRIO; LIPOSOME;

APOPTOSIS; ARTICLE; BILAYER MEMBRANE; CARBOXY TERMINAL SEQUENCE; ERYTHROCYTE; HEMOLYSIS; IMMUNOCOMPETENT CELL; INTESTINE CELL; NONHUMAN; PROTEIN DOMAIN; PROTEIN ISOLATION; PROTEIN TRANSPORT; VIBRIO CHOLERAE; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; DIFFUSION; GENETICS; METABOLISM; MICROBIOLOGY; MOLECULAR GENETICS; PHYSIOLOGY; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; RABBIT; ULTRASTRUCTURE;

AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; DIFFUSION; ERYTHROCYTES; HEMOLYSIN PROTEINS; HEMOLYSIS; LIPOSOMES; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RABBITS; VIBRIO CHOLERAE;

EID: 79952919239     PISSN: 09715916     EISSN: 09715916     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Kaper JB, Morris JG Jr, Levine MM. Cholera. Clin Microbiol Rev 1995; 8: 48-86.
2. Heuck AP, Tweten RK, Johnson AE. β-barrel pore-forming toxins: intriguing dimorphic proteins. Biochemistry 2001; 40: 9065-9073.
3. Tweten RK. Cholesterol-dependent cytolysins, a family of versatile pore-forming toxins. Infect Immun 2005; 73: 6199-6209.
4. Nagamune K, Yamamoto K, Naka A, Matsuyama J, Miwatani T, Honda T. In vitro proteolytic processing and activation of the recombinant precursor of El Tor cytolysin/hemolysin (pro-HlyA) of Vibrio cholerae by soluble hemagglutinin/protease of V. cholerae, trypsin and other proteases. Infect Immun 1996; 64: 4655-4658.
5. Krasilnikov OV, Muratkhodjaev JN, Zitzer AO. The mode of action of Vibrio cholerae cytolysin. The influences on both erythrocytes and planar lipid bilayers. Biochim Biophys Acta 1992; 1111: 7-16.
6. Ichinose Y, Yamamoto K, Nakasone N, Tanabe MJ, Takeda T, Miwatani T, et al. Enterotoxicity of El Tor-like hemolysin of non-O1 Vibrio cholerae. Infect Immun 1987; 55: 1090-1093.
7. Zitzer A, Wassenaar TM, Walev I, Bhakdi S. Potent membrane-permeabilizing and cytocidal action of Vibrio cholerae cytolysin on human intestinal cells. Infect Immun 1997; 65: 1293-1298.
8. Mitra R, Figueroa P, Mukhopadhyay AK, Shimada T, Takeda Y, Berg DE, et al. Cell vacuolation, a manifestation of the El Tor hemolysin of Vibrio cholerae. Infect Immun 2000; 68: 1928-1933.
9. Saka HA, Bidinost C, Sola C, Carranza P, Collino C, Ortiz S, et al. Vibrio cholerae cytolysin is essential for high enterotoxicity and apoptosis induction produced by a cholera toxin gene-negative V. cholerae non-O1, non-O139 strain. Microb Pathog 2008; 44: 118-128.
10. Mukherjee G, Biswas A, Banerjee KK, Biswas T. Vibrio cholerae hemolysin is apoptogenic to peritoneal B-1a cells but its oligomer shepherd the cells for IgA response. Mol Immunol 2008; 45: 266-270.
11. Rapoport TA, Jungnickel B, Kutay U. Protein transport across the eukaryotic endoplasmic reticulum and bacterial inner membranes. Annu Rev Biochem 1996; 65: 271-303.
12. Chattopadhyay K, Banerjee KK. Unfolding of Vibrio cholerae hemolysin induces oligomerization of the toxin monomer. J Biol Chem 2003; 278: 38470-38475.
13. Olson R, Gouaux E. Crystal structure of the Vibrio cholerae cytolysin (VCC) protoxin and its assembly into a heptameric transmembrane pore. J Mol Biol 2005; 350: 997-1016.
14. Saha N, Banerjee KK. Carbohydrate-mediated regulation of interaction of Vibrio cholerae hemolysin with erythrocyte and phospholipids vesicle. J Biol Chem 1997; 272: 162-167.
15. Chattopadhyay K, Bhattacharyya D, Banerjee KK. Vibrio cholerae hemolysin: Implication of amphiphilicity and lipid-induced conformational change for its pore-forming activity. Eur J Biochem 2002; 269: 4351-4358.
16. Zitzer A, Zitzer O, Bhakdi S, Palmer M. Oligomerization of Vibrio cholerae cytolysin yields a pentameric pore and has a dual specificity for cholesterol and sphingolipids in the target membrane. J Biol Chem 1999; 274: 1375-1380.
17. Dutta S, Mazumdar B, Banerjee KK, Ghosh AN. Three-dimensional structure of different functional forms of the Vibrio cholerae hemolysin oligomer: a cryo-electron microscopic study. J Bacteriol 2010; 192: 169-178.
18. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-685.
19. Markwell MA, Haas SM, Bieber LL, Tolbert NE. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem 1978; 87: 206-210.
20. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugar and related substances. Anal Chem 1956; 28: 350-356.
21. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979; 76: 4350-4354.
22. Zajchowski LD, Robbins SM. Lipid rafts and little caves: compartmentalized signalling in membrane microdomains. Eur J Biochem 2002; 269: 737-752.
23. Diep DB, Nelson KL, Raja SM, Pleshak EN, Buckley JT. Glycosylphosphatidylinositol anchors of membrane glycoproteins are binding determinants for the channel-forming toxin aerolysin. J Biol Chem 1998; 273: 2355-2360.