Expression pattern of Oct-4, sox2, and c-Myc in the primary culture of human dental pulp derived cells

Journal of Endodontics

Volumn 37, Issue 4, 2011, Pages 466-472

  Liu, Lu ^a,b   Wei, Xi ^a   Ling, Junqi ^a   Wu, Liping ^a   Xiao, Yin ^b  

^a SUN YAT SEN UNIVERSITY   (China)

^b QUEENSLAND UNIVERSITY OF TECHNOLOGY   (Australia)

Author keywords

Dental pulp cells (DPCs); explant culture; reprogramming markers

Indexed keywords

MYC PROTEIN; OCTAMER TRANSCRIPTION FACTOR 4; SOX2 PROTEIN, HUMAN; TRANSCRIPTION FACTOR SOX;

ADOLESCENT; ADULT; ARTICLE; CELL AGING; CELL CULTURE; CELL DIFFERENTIATION; CELL MOTION; CELL NUCLEUS; CELLULAR, SUBCELLULAR AND MOLECULAR BIOLOGICAL PHENOMENA AND FUNCTIONS; CHILD; CULTURE TECHNIQUE; CYTOLOGY; CYTOPLASM; FLUORESCENT ANTIBODY TECHNIQUE; GENE EXPRESSION REGULATION; GENETICS; HUMAN; MITOSIS; NUCLEAR REPROGRAMMING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STEM CELL; TIME; TOOTH PULP; ULTRASTRUCTURE;

ADOLESCENT; ADULT; CELL AGING; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL MOVEMENT; CELL NUCLEUS; CELL PHYSIOLOGICAL PROCESSES; CELLS, CULTURED; CHILD; CYTOPLASM; DENTAL PULP; FLUORESCENT ANTIBODY TECHNIQUE; GENE EXPRESSION REGULATION; HUMANS; MITOSIS; NUCLEAR REPROGRAMMING; OCTAMER TRANSCRIPTION FACTOR-3; PROTO-ONCOGENE PROTEINS C-MYC; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SOXB1 TRANSCRIPTION FACTORS; STEM CELLS; TIME FACTORS; YOUNG ADULT;

EID: 79952817038     PISSN: 00992399     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.joen.2010.12.012     Document Type: Article

References (31)

1. Wei X, Ling J, Wu L, Liu L, Xiao Y. Expression of mineralization markers in dental pulp stem cells. J Endod 2007;33:703-8.
2. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci 2000;97:13625-30.
3. Iohara K, Zheng L, Ito M, Tomokiyo A, Matsushita K, Nakashima M. Side population cells isolated from porcine dental pulp tissue with self-renewal and multipotency for dentinogenesis, chondrogenesis, adipogenesis, and neurogenesis. Stem Cells 2006;24:2493-503. (Pubitemid 44674310)
4. Mimeault M, Batra SK. Aging of tissue-resident adult stem/progenitor cells and their pathological consequences. Panminerva Med 2009;51:57-79.
5. Wagner W, Bork S, Horn P, et al. Aging and replicative senescence have related effects on human stem and progenitor cells. PLoS One 2009;4:e5846.
6. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006;126:663-76. (Pubitemid 44233629)
7. Yu J, Vodyanik MA, Smuga-Otto K, et al. Induced pluripotent stem cell lines derived from human somatic cells. Science 2007;318:1917-20.
8. Yan X, Qin H, Qu C, Tuan RS, Shi S, Huang GT. iPS cells reprogrammed from human mesenchymal-like stem/progenitor cells of dental tissue origin. Stem Cells Dev 2010;19:469-80.
9. Wei X, Wu L, Ling J, Liu L, Xiao Y. Differentially expressed protein profile of human dental pulp cells in early process of odontoblast-like differentiation in vitro. J Endod 2008;34:1077-84.
10. Liu L, Ling J, Wei X, Wu L, Xiao Y. Stem cell regulatory gene expression in human adult dental pulp and periodontal ligament cells undergoing odontogenic/osteogenic differentiation. J Endod 2009;35:1368-76.
11. Cortes O, Garcia C, Perez L, Boj J, Alcaina A. Pulp cell cultures obtained with two different methods for in vitro cytotoxicity tests. Eur Arch Paediatr Dent 2006;7:96-9.
12. Babaie Y, Herwig R, Greber B, et al. Analysis of Oct4-dependent transcriptional networks regulating self-renewal and pluripotency in human embryonic stem cells. Stem Cells 2007;25:500-10. (Pubitemid 46232806)
13. Lengner CJ, Welstead GG, Jaenisch R. The pluripotency regulator Oct4: a role in somatic stem cells? Cell Cycle 2008;7:725-8.
14. Lengner CJ, Camargo FD, Hochedlinger K, et al. Oct4 expression is not required for mouse somatic stem cell self-renewal. Cell Stem Cell 2007;1:403-15. (Pubitemid 47506536)
15. Kerkis I, Kerkis A, Dozortsev D, et al. Isolation and characterization of a population of immature dental pulp stem cells expressing OCT-4 and other embryonic stem cell markers. Cells Tissues Organs 2006;184:105-16.
16. Brambrink T, Foreman R, Welstead GG, et al. Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2008;2:151-9. (Pubitemid 351168714)
17. Loh YH, Wu Q, Chew JL, et al. The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells. Nat Genet 2006;38:431-40.
18. Boyer LA, Lee TI, Cole MF, et al. Core transcriptional regulatory circuitry in human embryonic stem cells. Cell 2005;122:947-56. (Pubitemid 41345211)
19. Lee J, Kim HK, Rho JY, et al. The human OCT-4 isoforms differ in their ability to confer self-renewal. J Biol Chem 2006;281:33554-65. (Pubitemid 46036736)
20. Ma D, Ma Z, Zhang X, et al. Effect of age and extrinsic microenvironment on the proliferation and osteogenic differentiation of rat dental pulp stem cells in vitro. J Endod 2009;35:1546-53.
21. Li J, Pan G, Cui K, Liu Y, Xu S, Pei D. A dominant-negative form of mouse SOX2 induces trophectoderm differentiation and progressive polyploidy in mouse embryonic stem cells. J Biol Chem 2007;282:19481-92. (Pubitemid 47100100)
22. Fong H, Hohenstein KA, Donovan PJ. Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells. Stem Cells 2008;26:1931-8.
23. Que J, Luo X, Schwartz RJ, Hogan BL. Multiple roles for Sox2 in the developing and adult mouse trachea. Development 2009;136:1899-907.
24. Singh AM, Dalton S. The cell cycle and Myc intersect with mechanisms that regulate pluripotency and reprogramming. Cell Stem Cell 2009;5:141-9.
25. Merrill BJ. Develop WNTs in somatic cell reprogramming. Cell Stem Cell 2008;3:465-6.
26. Wernig M, Meissner A, Cassady JP, Jaenisch R. c-Myc is dispensable for direct reprogramming of mouse fibroblasts. Cell Stem Cell 2008;2:10-2.
27. Takahashi K, Tanabe K, Ohnuki M, et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 2007;131:861-72. (Pubitemid 350138099)
28. Okada M, Oka M, Yoneda Y. Effective culture conditions for the induction of pluripotent stem cells. Biochim Biophys Acta 2010;1800:956-63.
29. Takemoto T, Uchikawa M, Kamachi Y, Kondoh H. Convergence of Wnt and FGF signals in the genesis of posterior neural plate through activation of the Sox2 enhancer N-1. Development 2006;133:297-306. (Pubitemid 43210718)
30. Mansukhani A, Ambrosetti D, Holmes G, Cornivelli L, Basilico C. Sox2 induction by FGF and FGFR2 activating mutations inhibits Wnt signaling and osteoblast differentiation. J Cell Biol 2005;168:1065-76.
31. Smith KP, Luong MX, Stein GS. Pluripotency: toward a gold standard for human ES and iPS cells. Cell Physiol 2009;220:21-9.