Enrichment of cardiac differentiation of mouse embryonic stem cells by optimizing the hanging drop method

Biotechnology Letters

Volumn 33, Issue 4, 2011, Pages 853-858

  Chen, Ming ^a   Lin, Yong Qing ^a   Xie, Shuang Lun ^a   Wu, Hong Fu ^b   Wang, Jing Feng ^a  

^a SUN YAT SEN MEMORIAL HOSPITAL OF SUN YAT SEN UNIVERSITY   (China)

^b GUANGDONG MEDICAL UNIVERSITY   (China)

Author keywords

Cardiomyocytes; Differentiation; Embryonic stem cells; Hanging drop method

Indexed keywords

CARDIAC DIFFERENTIATION; CARDIOMYOCYTES; CELL TYPES; DIFFERENTIATION; EMBRYOID BODY; EMBRYONIC STEM CELLS; HANGING DROP METHOD; MOUSE EMBRYONIC STEM CELLS;

CELL CULTURE; DROPS; HEART; OPTIMIZATION; STEM CELLS;

CELL GROWTH;

ANIMAL; ARTICLE; CELL DIFFERENTIATION; CULTURE TECHNIQUE; CYTOLOGY; EMBRYONIC STEM CELL; HEART MUSCLE CELL; IMMUNOHISTOCHEMISTRY; METHODOLOGY; MOUSE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; EMBRYONIC STEM CELLS; IMMUNOHISTOCHEMISTRY; MICE; MYOCYTES, CARDIAC; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 79952773286     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0494-3     Document Type: Article

References (14)

1. Abranches E, Bekman E, Henrique D, Cabral JM (2003) Expansion and neural differentiation of embryonic stem cells in adherent and suspension cultures. Biotechnol Lett 25: 725-730.
2. Boheler KR, Czyz J, Tweedie D, Yang HT, Anisimov SV, Wobus AM (2002) Differentiation of pluripotent embryonic stem cells into cardiomyocytes. Circ Res 91: 189-201.
3. Bongso A, Fong CY, Gauthaman K (2008) Taking stem cells to the clinic: major challenges. J Cell Biochem 105: 1352-1360.
4. Bratt-Leal AM, Carpenedo RL, McDevitt TC (2009) Engineering the embryoid body microenvironment to direct embryonic stem cell differentiation. Biotechnol Prog 25: 43-51.
5. Burridge PW, Anderson D, Priddle H, Barbadillo Munoz MD, Chamberlain S, Allegrucci C, Young LE, Denning C (2007) Improved human embryonic stem cell embryoid body homogeneity and cardiomyocyte differentiation from a novel V-96 plate aggregation system highlights interline variability. Stem Cells 25: 929-938.
6. Denning C, Allegrucci C, Priddle H, Barbadillo-Munoz MD, Anderson D, Self T, Smith NM, Parkin CT, Young LE (2006) Common culture conditions for maintenance and cardiomyocyte differentiation of the human embryonic stem cell lines, BG01 and HUES-7. Int J Dev Biol 50: 27-37.
7. Hakuno D, Takahashi T, Lammerding J, Lee RT (2005) Focal adhesion kinase signaling regulates cardiogenesis of embryonic stem cells. J Biol Chem 280: 39534-39544.
8. Hwang YS, Chung BG, Ortmann D, Hattori N, Moeller HC, Khademhosseini A (2009) Microwell-mediated control of embryoid body size regulates embryonic stem cell fate via differential expression of WNT5a and WNT11. Proc Natl Acad Sci USA 106: 16978-16983.
9. Lints TJ, Parsons LM, Hartley L, Lyons I, Harvey RP (1993) Nkx-2. 5: a novel murine homeobox gene expressed in early heart progenitor cells and their myogenic descendants. Development 119: 969.
10. Maltsev VA, Rohwedel J, Hescheler J, Wobus AM (1993) Embryonic stem cells differentiate in vitro into cardiomyocytes representing sinusnodal, atrial and ventricular cell types. Mech Dev 44: 41-50.
11. Maltsev VA, Wobus AM, Rohwedel J, Bader M, Hescheler J (1994) Cardiomyocytes differentiated in vitro from embryonic stem cells developmentally express cardiac-specific genes and ionic currents. Circ Res 75: 233-244.
12. Murry CE, Keller G (2008) Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development. Cell 132: 661-680.
13. Park J, Cho CH, Parashurama N, Li Y, Berthiaume F, Toner M, Tilles AW, Yarmush ML (2007) Microfabrication-based modulation of embryonic stem cell differentiation. Lab Chip 7: 1018-1028.
14. Wobus AM, Kaomei G, Shan J, Wellner MC, Rohwedel J, Ji G, Fleischmann B, Katus HA, Hescheler J, Franz WM (1997) Retinoic acid accelerates embryonic stem cell-derived cardiac differentiation and enhances development of ventricular cardiomyocytes. J Mol Cell Cardiol 29: 1525-1539.