Dynamic quantitative visualization of single cell alignment and migration and matrix remodeling in 3-D collagen hydrogels under mechanical force

Biomaterials

Volumn 32, Issue 15, 2011, Pages 3776-3783

  Pang, Yonggang ^a,b,c   Wang, Xiaoli ^b   Lee, Dongkeun ^c   Greisler, Howard P ^a,c  

^a LOYOLA UNIVERSITY MEDICAL CENTER   (United States)

^b Illinois Institute of Technology   (United States)

^c HINES VA HOSPITAL   (United States)

Author keywords

Cell signaling; Collagen; Confocal microscopy; ECM; Molecular imaging; Smooth muscle cell

Indexed keywords

ALIGNMENT DYNAMICS; CELL ALIGNMENT; CELL BODIES; CELL CONCENTRATIONS; CELL INTERFERENCE; CELL MATRIX INTERACTIONS; CELL MIGRATION; CELL SIGNALING; CELL SPREADING; CELL TRACKING; COLLAGEN MATRICES; DIFFERENTIAL INTERFERENCE CONTRAST; DYNAMIC VISUALIZATION; ECM; ENGINEERED TISSUES; HIGHLY INTEGRATED; IN-CELL; MATRIX; MATRIX REMODELING; MECHANICAL FORCE; MECHANICAL SIGNALS; MECHANICAL STRETCH; MICRO-SCALES; MOLECULAR IMAGING; PHASE-CONTRAST MICROSCOPY; QUANTITATIVE VISUALIZATION; SINGLE CELLS; SMOOTH MUSCLE CELL; SMOOTH MUSCLE CELLS;

COLLAGEN; CONFOCAL MICROSCOPY; CYTOLOGY; DYNAMICS; MECHANICAL ENGINEERING; MECHANICAL PROPERTIES; MUSCLE; SIGNALING; THREE DIMENSIONAL; VISUALIZATION;

CELLS;

COLLAGEN;

ANIMAL CELL; ARTICLE; CELL ASSAY; CELL INTERACTION; CELL MIGRATION; CELL SPREADING; CELL STRUCTURE; CELL TRACKING; CONFOCAL MICROSCOPY; EXTRACELLULAR MATRIX; FLUORESCENCE; HYDROGEL; IMAGING SYSTEM; MOLECULAR DYNAMICS; NONHUMAN; PHASE CONTRAST MICROSCOPE; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; SMOOTH MUSCLE FIBER; THREE DIMENSIONAL IMAGING;

ANIMALS; CAROTID ARTERIES; CATTLE; CELL MOVEMENT; CELL SURVIVAL; CELLS, CULTURED; COLLAGEN; DOGS; EQUIPMENT DESIGN; EXTRACELLULAR MATRIX; HYDROGELS; MECHANICAL PHENOMENA; MICROSCOPY, CONFOCAL; MYOCYTES, SMOOTH MUSCLE; SINGLE-CELL ANALYSIS; TISSUE SCAFFOLDS;

EID: 79952766931     PISSN: 01429612     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2011.02.003     Document Type: Article

References (31)

1. Engler A.J., Sen S., Sweeney H.L., Discher D.E. Matrix elasticity directs stem cell lineage specification. Cell 2006, 126:677-689.
2. Ingber D.E. Mechanical control of tissue morphogenesis during embryological development. Int J Dev Biol 2006, 50:255-266.
3. le Noble F., Klein C., Tintu A., Pries A., Buschmann I. Neural guidance molecules, tip cells, and mechanical factors in vascular development. Cardiovasc Res 2008, 78:232-241.
4. Lim C.T., Zhou E.H., Quek S.T. Mechanical models for living cells-a review. J Biomech 2006, 39:195-216.
5. Haga J.H., Li Y.S., Chien S. Molecular basis of the effects of mechanical stretch on vascular smooth muscle cells. J Biomech 2007, 40:947-960.
6. Yim E.K., Reano R.M., Pang S.W., Yee A.F., Chen C.S., Leong K.W. Nanopattern-induced changes in morphology and motility of smooth muscle cells. Biomaterials 2005, 26:5405-5413.
7. Kaunas R., Nguyen P., Usami S., Chien S. Cooperative effects of Rho and mechanical stretch on stress fiber organization. Proc Natl Acad Sci U S A 2005, 102:15895-15900.
8. Reno F., Traina V., Cannas M. Mechanical stretching modulates growth direction and MMP-9 release in human keratinocyte monolayer. Cell Adh Migr 2009, 3:239-242.
9. Hayakawa K., Sato N., Obinata T. Dynamic reorientation of cultured cells and stress fibers under mechanical stress from periodic stretching. Exp Cell Res 2001, 268:104-114.
10. Cukierman E., Pankov R., Stevens D.R., Yamada K.M. Taking cell-matrix adhesions to the third dimension. Science 2001, 294:1708-1712.
11. Keller P.J., Pampaloni F., Stelzer E.H. Life sciences require the third dimension. Curr Opin Cell Biol 2006, 18:117-124.
12. Pampaloni F., Reynaud E.G., Stelzer E.H. The third dimension bridges the gap between cell culture and live tissue. Nat Rev Mol Cell Biol 2007, 8:839-845.
13. Bell E., Ivarsson B., Merrill C. Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. Proc Natl Acad Sci U S A 1979, 76:1274-1278.
14. Bellows C.G., Melcher A.H., Aubin J.E. Association between tension and orientation of periodontal ligament fibroblasts and exogenous collagen fibres in collagen gels in vitro. J Cell Sci 1982, 58:125-138.
15. Huang D., Chang T.R., Aggarwal A., Lee R.C., Ehrlich H.P. Mechanisms and dynamics of mechanical strengthening in ligament-equivalent fibroblast-populated collagen matrices. Ann Biomed Eng 1993, 21:289-305.
16. Roby T., Olsen S., Nagatomi J. Effect of sustained tension on bladder smooth muscle cells in three-dimensional culture. Ann Biomed Eng 2008, 36:1744-1751.
17. Berry C.C., Shelton J.C., Lee D.A. Cell-generated forces influence the viability, metabolism and mechanical properties of fibroblast-seeded collagen gel constructs. J Tissue Eng Regen Med 2009, 3:43-53.
18. Lee E.J., Holmes J.W., Costa K.D. Remodeling of engineered tissue anisotropy in response to altered loading conditions. Ann Biomed Eng 2008, 36:1322-1334.
19. Pang Y., Ucuzian A.A., Matsumura A., Brey E.M., Gassman A.A., Husak V.A., et al. The temporal and spatial dynamics of microscale collagen scaffold remodeling by smooth muscle cells. Biomaterials 2009, 30:2023-2031.
20. Pang Y., Wang X., Ucuzian A.A., Brey E.M., Burgess W.H., Jones K.J., et al. Local delivery of a collagen-binding FGF-1 chimera to smooth muscle cells in collagen scaffolds for vascular tissue engineering. Biomaterials 2010, 31:878-885.
21. Pang Y., Greisler H.P. Using a type 1 collagen-based system to understand cell-scaffold interactions and to deliver chimeric collagen-binding growth factors for vascular tissue engineering. J Investig Med 2010, 58:845-848.
22. Ziegler T., Nerem R.M. Tissue engineering a blood vessel: regulation of vascular biology by mechanical stresses. J Cell Biochem 1994, 56:204-209.
23. Mammoto T., Ingber D.E. Mechanical control of tissue and organ development. Development 2010, 137:1407-1420.
24. Dado D., Levenberg S. Cell-scaffold mechanical interplay within engineered tissue. Semin Cell Dev Biol 2009, 20:656-664.
25. Gerlich D., Ellenberg J. 4D imaging to assay complex dynamics in live specimens. Nat Cell Biol 2003, Suppl:S14-S19.
26. Waters J.C. Live-cell fluorescence imaging. Methods Cell Biol 2007, 81:115-140.
27. Gibbons G.H., Dzau V.J. The emerging concept of vascular remodeling. N Engl J Med 1994, 330:1431-1438.
28. Li L., Chaikof E.L. Mechanical stress regulates syndecan-4 expression and redistribution in vascular smooth muscle cells. Arterioscler Thromb Vasc Biol 2002, 22:61-68.
29. Li C., Wernig F., Leitges M., Hu Y., Xu Q. Mechanical stress-activated PKCdelta regulates smooth muscle cell migration. FASEB J 2003, 17:2106-2108.
30. Libby P., Tanaka H. The molecular bases of restenosis. Prog Cardiovasc Dis 1997, 40:97-106.
31. Newby A.C., Zaltsman A.B. Molecular mechanisms in intimal hyperplasia. J Pathol 2000, 190:300-309.