Synthesis and properties of immobilized pectinase onto the macroporous polyacrylamide microspheres

Journal of Agricultural and Food Chemistry

Volumn 59, Issue 6, 2011, Pages 2592-2599

  Lei, Zhongli ^a   Jiang, Qin ^a  

^a SHAANXI NORMAL UNIVERSITY   (China)

Author keywords

Covalent immobilization; large surface area; macroporous polyacrylamide (PAM) microspheres; pectinase

Indexed keywords

BATCH REACTIONS; CARRIER PARTICLES; COVALENT IMMOBILIZATION; IMMOBILIZED ENZYME; INITIAL ACTIVITY; INVERSE SUSPENSION POLYMERIZATION; LARGE SURFACE AREA; MACROPOROUS; MODEL REACTIONS; OPERATIONAL STABILITY; PECTINASES; POLYACRY LAMIDE; POLYACRYLAMIDES; RESIDUAL ACTIVITY;

CENTRIFUGATION; MICROSPHERES; REACTION KINETICS; SYNTHESIS (CHEMICAL);

ENZYMES;

ACRYLIC ACID RESIN; IMMOBILIZED ENZYME; MICROSPHERE; POLYACRYLAMIDE; POLYGALACTURONASE;

ADSORPTION; ARTICLE; CHEMISTRY; ENZYME STABILITY; KINETICS; METABOLISM; POROSITY; TEMPERATURE;

ACRYLIC RESINS; ADSORPTION; ENZYME STABILITY; ENZYMES, IMMOBILIZED; KINETICS; MICROSPHERES; POLYGALACTURONASE; POROSITY; TEMPERATURE;

EID: 79952765123     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf103719t     Document Type: Article

References (28)

1. Kashyap, D. R.; Vohra, P. K.; Chopra, S.; Tewari, R. Applications of pectinases in the commercial sector: A review Bioresour. Technol. 2001, 77, 215-227 (Pubitemid 32015866)
2. Alkorta, I.; Garbisu, C.; Llama, M. J.; Serra, J. L. Industrial applications of pectic enzymes Process Biochem. 1998, 33, 21-28
3. Sheldon, R. A. Enzyme immobilization: The quest for optimum performance Adv. Synth. Catal. 2007, 349, 1289-1307
4. Mateo, C.; Palomo, J. M.; Fernandez-Lorente, G.; Guisan, J. M.; Fernandez-Lafuente, R. Improvement of enzyme activity, stability and via immobilization techniques Enzyme Microb. Technol. 2007, 40, 1451-1463 (Pubitemid 46482654)
5. Jin, W.; Brennan, J. D. Properties and applications of proteins encapsulated within sol-gel derived materials Anal. Chim. Acta 2002, 461, 1-36 (Pubitemid 34603555)
6. +2 Anal. Chem. 2005, 77, 2670-2672 (Pubitemid 40562773)
7. Lei, Z.; Bi, S. Preparation and properties of immobilized pectinase onto the amphiphilic PS- b -PAA diblock copolymers J. Biotechnol. 2007, 128, 112-119 (Pubitemid 44960515)
8. 2- g -PSStNa polymer nanocomposites microspheres (PNCMs) from a surface-initiated atom transfer radical polymerization (SI-ATRP) approach for pectinase immobilization J. Agric. Food Chem. 2009, 57, 1544-1549
9. Liu, C.; Ohta, H.; Kuwahara, T.; Shimomura, M. Amperometric glucose-responding property of enzyme electrodes fabricated by covalent immobilization of glucose oxidase on conducting polymer films with macroporous structure Eur. Polym. J. 2008, 44, 1114-1122
10. Li, Y.; Gao, F.; Wei, W.; Qu, J. B.; Ma, G. H.; Zhou, W. Q. Pore size of macroporous polystyrene microspheres affects lipase immobilization J. Mol. Catal. B: Enzym. 2010, 66, 182-189
11. Blanco, R. M.; Terreros, P.; Fernandez-Perez, M.; Otero, C.; Diaz-Gonzalez, G. Functionalization of mesoporous silica for lipase immobilisation characterization of the support and the catalysts J. Mol. Catal. B: Enzym. 2004, 30, 83-93 (Pubitemid 38597105)
12. Das, R. D.; Maji, S.; Das, S.; Chaudhuri, C. R. Optimization of covalent antibody immobilization on macroporous silicon solid supports Appl. Surf. Sci. 2010, 256, 5867-5875
13. Keeling-Tucker, T.; Brennan, J. D. Fluorescent probes as reporters on the local structure and dynamics in sol-gel-derived nanocomposite materials Chem. Mater. 2001, 13, 3331-3350 (Pubitemid 32999279)
14. Tsai, H. C.; Doong, R. A. Preparation and characterization of urease-encapsulated biosensors in poly(vinyl alcohol)-modified silica sol-gel materials Biosens. Bioelectron. 2007, 23, 66-73
15. Liu, P.; Guo, J. Polyacrylamide grafted attapulgite (PAM-ATP) via surface-initiated atom transfer radical polymerization (SI-ATRP) for removal of Hg(II) ion and dyes Colloids Surf., A 2006, 282-283, 498-503 (Pubitemid 43816841)
16. Tang, Z. G.; Zhou, R. Q.; Duan, Z. T. Adsorption and desorption behaviour of taurine on macroporous adsorption resins J. Chem. Technol. Biotechnol. 2001, 76, 752-756
17. Pessela, B. C. C.; Fernández-Lafuente, R.; Fuentes, M.; Vián, A.; Garcia, J. L.; Carrascosa, A. V.; Mateo, C.; Guisán, J. M. Reversible immobilization of a thermophilic β-galactosidase via ionic adsorption on PEI-coated Sepabeads Enzyme Microb. Technol. 2003, 32, 369-374 (Pubitemid 36136056)
18. Milosavíc, N.; Prodanovíc, R.; Jovanovíc, S.; Vujčic, Z. Immobilization of glucoamylase on macroporous spheres Acta Period. Technol. 2004, 35, 207-14
19. Fernandez-Lafuente, R. Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation Enzyme Microb. Technol. 2009, 45, 405-418
20. Walt, D. R.; Agayn, V. I. The chemistry of enzyme and protein immobilization with glutaraldehyde Trends Anal. Chem. 1994, 13, 425-430 (Pubitemid 24340111)
21. Migneault, I.; Dartiguenave, C.; Bertrand, M. J.; Waldron, K. C. Glutaraldehyde: Behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking BioTechniques 2004, 37, 798-802
22. Lowry, O. H.; Rosebrough, N. J.; Farr, A. L.; Randall, R. J. Protein measurement with the folin phenol reagent J. Biol. Chem. 1951, 193, 265-275
23. Miller, G. L. Use of dinitrosalicylic acid reagent for determination of reducing sugar Anal. Chem. 1959, 31, 426-428
24. Durán, N.; Rosa, M. A.; D'Annibale, A.; Gianfreda, L. Applications of laccases and tyrosinases (phenoloxidases) immobilized on different supports: A review Enzyme Microb. Technol. 2002, 31, 907-931 (Pubitemid 35375750)
25. Li, T. P.; Wang, N.; Zhao, Q. C.; Guo, M.; Zhang, C. Y. Optimization of covalent immobilization of pectinase on sodium alginate support Biotechnol. Lett. 2007, 29, 1413-1416 (Pubitemid 47205856)
26. Li, T.; Li, S.; Wang, N.; Tain, L. Immobilization and stabilization of pectinase by multipoint attachment onto an activated agar-gel support Food Chem. 2008, 109, 703-708
27. Li, Y.; Zhong, H.; Xiao, Y.; Ge, C.; Guo, H. Immobilization of pectinase on chitosan Food Sci. 2002, 23, 50-53 (in Chinese)
28. Roy, I.; Sardar, M.; Gupta, M. N. Evaluation of a smart bioconjugate of pectinase for chitin hydrolysis Biochem. Eng. J. 2003, 16, 329-335 (Pubitemid 37288391)