Validation of an automated method for salivary alpha-amylase measurements in pigs (Sus scrofa domesticus) and its application as a stress biomarker

Journal of Veterinary Diagnostic Investigation

Volumn 23, Issue 2, 2011, Pages 282-287

  Fuentes, María ^a   Tecles, Fernando ^a   Gutiérrez, Ana ^a   Otal, Julio ^a   Martínez Subiela, Silvia ^a   Cerón, José J ^a  

^a UNIVERSITY OF MURCIA   (Spain)

Author keywords

Alpha amylase; Pigs; Saliva; Stress

Indexed keywords

SUIDAE; SUS SCROFA DOMESTICA;

EID: 79952578310     PISSN: 10406387     EISSN: None     Source Type: Journal    
DOI: 10.1177/104063871102300213     Document Type: Article

References (28)

1. Candiani D, Salamano G, Mellia E, et al.: 2008, A combination of behavioral and physiological indicators for assessing pig welfare on the farm. J Appl Anim Welf Sci 11:1-13.
2. Chaloupková H, Illmann G, Neuhauserová K, et al.: 2007, Preweaning housing effects on behavior and physiological measures in pigs during the suckling and fattening periods. J Anim Sci 85:1741-1749.
3. DeCaro JA: 2008, Methodological considerations in the use of salivary a-amylase as a stress marker in field research. Am J Hum Biol 20:617-619.
4. Fazio E, Ferlazzo A: 2003, Evaluation of stress during transport. Vet Res Commun 27:519-524.
5. Geverink NA, Schoutenb WGP, Gortc G, Wiegant VM: 2002, Individual differences in behavioral and physiological responses to restraint stress in pigs. Physiol Behav 77:451-457.
6. Granger DA, Kivlighan KT, el-Sheikh M, et al.: 2007, Salivary alpha-amylase in biobehavioral research: recent developments and applications. Ann N Y Acad Sci 1098:122-144.
7. Gutiérrez AM, Martínez-Subiela S, Cerón JJ: 2009, Evaluation of an immunoassay for determination of haptoglobin concentration in various biological specimens from swine. Am J Vet Res 70:691-696.
8. Gutiérrez AM, Martínez-Subiela S, Eckersall PD, Cerón JJ: 2009, C-reactive protein quantification in porcine saliva: a minimally invasive test for pig health monitoring. Vet J 181:261-265.
9. Guzik AC, Matthews JO, Kerr BJ, et al.: 2006, Dietary tryptophan effects on plasma and salivary cortisol and meat quality in pigs. J Anim Sci 84:2251-2259.
10. International Federation of Clinical Chemistry, Scientific Division, Working Group on Enzymes: 1999, IFCC methods for measurement of catalytic concentration of enzymes. Part 9. IFCC method for alpha-amylase [1,4-a-D-glucan 4- glucanohydrolase, EC 3.2.1.1]. Clin Chim Acta 281:S5-S39.
11. Kawada S, Fukusaki C, Ohtani M, Kobayashi K: 2009, Effects of hyperoxic inhalation on psychological stressinduced salivary biomarkers. Biomed Res 30:245-249.
12. Klont RE, Hulsegge B, Hoving-Bolink AH, et al.: 2001, Relationships between behavioral and meat quality characteristics of pigs raised under barren and enriched housing conditions. J Anim Sci 79:2835-2843.
13. Koopmans SJ, Ruis M, Dekker R, et al.: 2005, Surplus dietary tryptophan reduces plasma cortisol and noradrenaline concentrations and enhances recovery after social stress in pigs. Physiol Behav 85:469-478.
14. Nater UM, Rohleder N: 2009, Salivary alpha-amylase as a non-invasive biomarker for the sympathetic nervous system: current state of research. Psychoneuroendocrinology 34:486-496.
15. Nater UM, Rohleder N, Gaab J, et al.: 2005, Human salivary alpha-amylase reactivity in a psychosocial stress paradigm. Int J Psychophysiol 55:333-342.
16. Ng V, Koh D, Wee A: 2008, Salivary alpha amylase levels under conditions of extreme examination stress. Psychol Rep 103:455-458.
17. Noto Y, Sato T, Kudo M, et al.: 2005, The relationship between salivary biomarkers and state-trait anxiety inventory score under mental arithmetic stress: a pilot study. Anesth Analg 101:1873-1876.
18. Rohleder N, Nater UM: 2009, Determinants of salivary alpha-amylase in humans and methodological considerations. Psychoneuroendocrinology 34:469-485.
19. Roozen AW, Tsuma VT, Magnusson U: 1995, Effects of short-term restraint stress on plasma concentrations of catecholamines, beta-endorphin, and cortisol in gilts. Am J Vet Res 56:1225-1227.
20. Rushen J: 1996, Using aversion learning techniques to assess the mental state, suffering, and welfare of farm animals. J Anim Sci 74:1990-1995.
21. Rushen J, Ladewig J: 1991, Stress-induced hypoalgesia and opioid inhibition of pigs' responses to restraint. Physiol Behav 50:1093-1096.
22. Takai N, Yamaguchi M, Aragaki T, et al.: 2004, Effect of psychological stress on the salivary cortisol and amylase levels in healthy young adults. Arch Oral Biol 49:963-968.
23. Tecles F, Fuentes P, Martínez Subiela S, et al.: 2007, Analytical validation of commercially available methods for acute phase proteins quantification in pigs. Res Vet Sci 83:133-139.
24. Tecles F, Martínez-Subiela S, Petrucci G, et al.: 2006, Validation of a commercially available human immunoturbidimetric assay for haptoglobin determination in canine serum samples. Vet Res Commun 31:23-36.
25. Van Stegeren AH, Wolf OT, Kindt M: 2008, Salivary alpha amylase and cortisol responses to different stress tasks: impact of sex. Int J Psychophysiol 69:33-40.
26. Wolf JM, Nicholls E, Chen E: 2008, Chronic stress, salivary cortisol, and alpha-amylase in children with asthma and healthy children. Biol Psychol 78:20-28.
27. Wong DT: 2006, Salivary diagnostics powered by nanotechnologies, proteomics and genomics. J Am Dent Assoc 137:313-321.
28. Yamaguchi M, Kanemaru M, Kanemori T, Mizuno Y: 2003, Flow-injection-type biosensor system for salivary amylase activity. Biosens Bioelectron 18:835-840.