Cryopreservation of human embryonic stem cells derived-cardiomyocytes induced by BMP2 in serum-free condition

Reproductive Sciences

Volumn 18, Issue 3, 2011, Pages 252-260

  Kim, Yoon Young ^a   Ku, Seung Yup ^a   Liu, Hung Ching ^b   Cho, Hyun Jai ^a   Oh, Sun Kyung ^a   Moon, Shin Yong ^a   Choi, Young Min ^a  

^a Seoul National University College of Medicine   (South Korea)

^b WEILL CORNELL MEDICAL COLLEGE   (United States)

Author keywords

BMP2; Cardiomyocytes; Cryopreservation; Human embryonic stem cells; Serum free differentiation

Indexed keywords

4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE; ACTIVIN A; BONE MORPHOGENETIC PROTEIN 2; ION CHANNEL; TRANSCRIPTION FACTOR NKX2.5;

ARTICLE; CELL DIFFERENTIATION; CELL LINEAGE; CELL SURVIVAL; CONTROLLED STUDY; CRYOPRESERVATION; CULTURE MEDIUM; EMBRYONIC STEM CELL; FREEZE THAWING; GENE EXPRESSION; HEART MUSCLE CELL; HUMAN; HUMAN CELL; MESODERM; PRIORITY JOURNAL; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

AMIDES; BONE MORPHOGENETIC PROTEIN 2; CELL DIFFERENTIATION; CELL LINE; CRYOPRESERVATION; EMBRYONIC STEM CELLS; ENZYME INHIBITORS; HUMANS; IMMUNOHISTOCHEMISTRY; MYOCYTES, CARDIAC; PYRIDINES;

EID: 79952510516     PISSN: 19337191     EISSN: 19337205     Source Type: Journal    
DOI: 10.1177/1933719110385130     Document Type: Article

References (27)

1. Thomson JA, Itskovitz-Eldor J, Shapiro SS, et al. Embryonic stem cell lines derived from human blastocysts. Science. 1998;282(5391):1145-1147. (Pubitemid 28516247)
2. Oh SK, Kim HS, Ahn HJ, et al. Derivation and characterization of new human embryonic stem cell lines: SNUhES1, SNUhES2, and SNUhES3. Stem Cells. 2005;23(2):211-219.
3. Sugi Y, Lough J. Activin-A and FGF-2 mimic the inductive effects of anterior endoderm on terminal cardiac myogenesis in vitro. Dev Biol. 1995;168(2):567-574.
4. Schultheiss TM, Burch JB, Lassar AB. A role for bone morphogenetic proteins in the induction of cardiac myogenesis. Genes Dev. 1997;11(4):451-462. (Pubitemid 27097644)
5. Laflamme MA, Chen KY, Naumova AV, et al. Cardiomyocytes derived from human embryonic stem cells in pro-survival factors enhance function of infarcted rat hearts. Nat Biotechnol. 2007;25(9):1015-1024. (Pubitemid 47517637)
6. Zhang P, Li J, Tan Z, et al. Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells. Blood. 2008;111(4):1933-1941. (Pubitemid 351451502)
7. Yang L, Soonpaa MH, Adler ED, et al. Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population. Nature. 2008;453(7194):524-528. (Pubitemid 351733312)
8. Tomescot A, Leschik J, Bellamy V, et al. Differentiation in vivo of cardiac committed human embryonic stem cells in postmyocardial infarcted rats. Stem Cells. 2007;25(9):2200-2205. (Pubitemid 47480478)
9. Pal R, Khanna A. Similar pattern in cardiac differentiation of human embryonic stem cell lines, BG01V and ReliCellhES1, under low serum concentration supplemented with bone morphogenetic protein-2. Differentiation. 2007;75(2):112-122.
10. Kim YY, Ku SY, Jang J, et al. Use of long-term cultured embryoid bodies may enhance cardiomyocyte differentiation by BMP2. Yonsei Med J. 2008;49(5):819-827.
11. Kehat I, Kenyagin-Karsenti D, Snir M, et al. Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes. J Clin Invest. 2001;108(3):407-414. (Pubitemid 32730787)
12. Xu C, Police S, Rao N, Carpenter MK. Characterization and enrichment of cardiomyocytes derived from human embryonic stem cells. Circ Res. 2002;91(6):501-508.
13. Itskovitz-Eldor J, Schuldiner M, Karsenti D, et al. Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers. Mol Med. 2000;6(2):88-95.
14. Keller GM. In vitro differentiation of embryonic stem cells. Curr Opin Cell Biol. 1995;7(6):862-869.
15. Li T, Zhou C, Liu C, Mai Q, Zhuang G. Bulk vitrification of human embryonic stem cells. Hum Reprod. 2008;23(2):358-364. (Pubitemid 351651259)
16. Meryman HT. Cryopreservation of living cells: principles and practice. Transfusion. 2007;47(5):935-945. (Pubitemid 46669010)
17. Li X, Meng G, Krawetz R, Liu S, Rancourt DE. The ROCK inhibitor Y-27632 enhances the survival rate of human embryonic stem cells following cryopreservation. Stem Cells Dev. 2008;17(6):1079-1085.
18. Watanabe K, Ueno M, Kamiya D, et al. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol. 2007;25(6):681-686. (Pubitemid 46897207)
19. Li X, Krawetz R, Liu S, Meng G, Rancourt DE. ROCK inhibitor improves survival of cryopreserved serum/feeder-free single human embryonic stem cells. Hum Reprod. 2009;24(3):580-589.
20. Mollamohammadi S, Taei A, Pakzad M, et al. A simple and efficient cryopreservation method for feeder-free dissociated human induced pluripotent stem cells and human embryonic stem cells. Hum Reprod. 2009;24(10):2468-2476.
21. Martin-Ibanez R, Unger C, Stromberg A, Baker D, Canals JM, Hovatta O. Novel cryopreservation method for dissociated human embryonic stem cells in the presence of a ROCK inhibitor. Hum Reprod. 2008;23(12):2744-2754.
22. Norstrom A, Akesson K, Hardarson T, Hamberger L, Bjorquist P, Sartipy P. Molecular and pharmacological properties of human embryonic stem cell-derived cardiomyocytes. Exp Biol Med (Maywood). 2006;231(11):1753-1762. (Pubitemid 44865994)
23. Oh SK, Kim HS, Park YB, et al. Methods for expansion of human embryonic stem cells. Stem Cells. 2005;23(5):605-609.
24. He JQ, Ma Y, Lee Y, Thomson JA, Kamp TJ. Human embryonic stem cells develop into multiple types of cardiac myocytes: action potential characterization. Circ Res. 2003;93(1):32-39. (Pubitemid 36859038)
25. Kehat I, Khimovich L, Caspi O, et al. Electromechanical integration of cardiomyocytes derived from human embryonic stem cells. Nat Biotechnol. 2004;22(10):1282-1289. (Pubitemid 39341201)
26. Jang J, Ku SY, Kim JE, et al. Notch inhibition promotes human embryonic stem cell-derived cardiac mesoderm differentiation. Stem Cells. 2008;26(11):2782-2790.
27. Heng BC, Ye CP, Liu H, et al. Loss of viability during freeze-thaw of intact and adherent human embryonic stem cells with conventional slow-cooling protocols is predominantly due to apoptosis rather than cellular necrosis. J Biomed Sci. 2006;13(3):433-445. (Pubitemid 43725258)