A modified protocol to prepare seed-free starting solutions of amyloid-β (Aβ)1-40 and Aβ1-42 from the corresponding depsipeptides

Analytical Biochemistry

Volumn 411, Issue 2, 2011, Pages 297-299

  Beeg, Marten ^a,b   Stravalaci, Matteo ^a   Bastone, Antonio ^a   Salmona, Mario ^a   Gobbi, Marco ^a  

^a MARIO NEGRI INSTITUTE FOR PHARMACOLOGICAL RESEARCH   (Italy)

^b ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

AGGREGATES; DICHROISM; GLYCOPROTEINS;

AMYLOID BETAS; AMYLOIDOGENIC PEPTIDES; BASIC CONDITIONS; CRITICAL STEPS; DEPSIPEPTIDES; NATIVE SEQUENCES; STARTING SOLUTIONS; STOCK SOLUTIONS;

PEPTIDES;

AMYLOID BETA PROTEIN[1-40]; AMYLOID BETA PROTEIN[1-42]; DEPSIPEPTIDE; THIOFLAVINE;

ARTICLE; CIRCULAR DICHROISM; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN STABILITY;

EID: 79952453932     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.12.032     Document Type: Article

References (23)

1. J. Hardy, and D.J. Selkoe The amyloid hypothesis of Alzheimer's disease: progress and problems on the road to therapeutics Science 297 2002 353 356 (Pubitemid 34790756)
2. G. Thinakaran, and E.H. Koo Amyloid precursor protein trafficking processing and function J. Biol. Chem. 283 2008 29615 29619
3. J.D. Harper, and P.T. Lansbury Jr. Models of amyloid seeding in Alzheimer's disease and scrapie: mechanistic truths and physiological consequences of the time-dependent solubility of amyloid proteins Annu. Rev. Biochem. 66 1997 385 407 (Pubitemid 27274662)
4. A.F. Henson, J.R. Mitchell, and P.R. Musselwhite The surface coagulation of proteins during shaking J. Colloid Interface Sci. 32 1970 162 165
5. N.I. Topilina, V.V. Ermolenkov, S. Higashiya, J.T. Welch, and I.K. Lednev β-Sheet folding of 11-kDa fibrillogenic polypeptide is completely aggregation driven Biopolymers 86 2007 261 264 (Pubitemid 47067542)
6. B. O'Nuallain, A.K. Thakur, A.D. Williams, A.M. Bhattacharyya, S. Chen, G. Thiagarajan, and R. Wetzel Kinetics and thermodynamics of amyloid assembly using a high-performance liquid chromatography-based sedimentation assay Methods Enzymol. 413 2006 34 74 (Pubitemid 44528684)
7. D.B. Teplow Preparation of amyloid-β protein for structural and functional studies Methods Enzymol. 413 2006 20 33 (Pubitemid 44528683)
8. Y. Fezoui, D.M. Hartley, J.D. Harper, R. Khurana, D.M. Walsh, M.M. Condron, D.J. Selkoe, P.T. Lansbury Jr., A.L. Fink, and D.B. Teplow An improved method of preparing the amyloid-β protein for fibrillogenesis and neurotoxicity experiments Amyloid 7 2000 166 178
9. Y. Sohma, T. Yoshiya, A. Taniguchi, T. Kimura, Y. Hayashi, and Y. Kiso Development of O-acyl isopeptide method Biopolymers 88 2007 253 262 (Pubitemid 46657991)
10. 1-42 via O-N intramolecular acyl migration reaction Tetrahedron Lett. 45 2004 5965 5968 (Pubitemid 38916534)
11. 1-42 Tetrahedron Lett. 45 2004 7519 7523 (Pubitemid 39208399)
12. S. Dos Santos, A. Chandravarkar, B. Mandal, R. Mimna, K. Murat, L. Saucede, P. Tella, G. Tuchscherer, and M. Mutter Switch-peptides: controlling self-assembly of amyloid β-derived peptides in vitro by consecutive triggering of acyl migrations J. Am. Chem. Soc. 127 2005 11888 11889 (Pubitemid 41233685)
13. Y. Sohma, A. Taniguchi, M. Skwarczynski, T. Yoshiya, F. Fukao, T. Kimura, Y. Hayashi, and Y. Kiso O-acyl isopeptide method for the efficient synthesis of difficult sequence-containing peptides: use of O-acyl isodipeptide unit Tetrahedron Lett. 47 2006 3013 3017
14. I. Coin, R. Dölling, E. Krause, M. Bienert, M. Beyermann, C.D. Sferdean, and L.A. Carpino Depsipeptide methodology for solid-phase peptide synthesis: Circumventing side reactions and development of an automated technique via depsidipeptide units J. Org. Chem. 71 2006 6171 6177 (Pubitemid 44200288)
15. T. Yoshiya, A. Taniguchi, Y. Sohma, F. Fukao, S. Nakamura, N. Abe, N. Ito, M. Skwarczynski, T. Kimura, Y. Hayashi, and Y. Kiso O-acyl isopeptide method" for peptide synthesis: Synthesis of 40 kinds of O-acyl isodipeptide unit" Boc-Ser/Thr(Fmoc-Xaa)-OH Org. Biomol. Chem. 5 2007 1720 1730 (Pubitemid 46794162)
16. 1-42 ChemBioChem 10 2009 710 715
17. 1-42 are potent central nervous system neurotoxins Proc. Natl. Acad. Sci. USA 95 1998 6448 6453
18. W.B. Stine Jr., K.N. Dahlgren, G.A. Krafft, and M.J. LaDu In vitro characterization of conditions for amyloid-β peptide oligomerization and fibrillogenesis J. Biol. Chem. 278 2003 11612 11622 (Pubitemid 36792723)
19. 1-42 J. Pept. Sci. 11 2005 441 451 (Pubitemid 41086924)
20. J. Bieschke, S.J. Siegel, Y. Fu, and J.W. Kelly Alzheimer's Aβ peptides containing an isostructural backbone mutation afford distinct aggregate morphologies but analogous cytotoxicity: evidence for a common low-abundance toxic structure(s)? Biochemistry 47 2008 50 59
21. A.T. Petkova, R.D. Leapman, Z. Guo, W.M. Yau, M.P. Mattson, and R. Tycko Self-propagating molecular-level polymorphism in Alzheimer's β-amyloid fibrils Science 307 2005 262 265 (Pubitemid 40116242)
22. M. Yang, and D.B. Teplow Amyloid-β protein monomer folding: free-energy surfaces reveal alloform-specific differences J. Mol. Biol. 384 2008 450 464
23. 1-42 peptide self-aggregation: elucidation of inhibitors' mechanism of action ChemBioChem 8 2007 2152 2161 (Pubitemid 350220767)