Evidence of epithelial to mesenchymal transition associated with increased tumorigenic potential in an immortalized normal prostate epithelial cell line

Prostate

Volumn 71, Issue 6, 2011, Pages 626-636

  Marian, Calin O ^a   Yang, Lin ^b,c   Zou, Ying S ^d   Gore, Crystal ^b   Pong, Rey Chen ^b   Shay, Jerry W ^a   Kabbani, Wareef ^b   Hsieh, Jer Tsong ^b   Raj, Ganesh V ^b  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^c XI'AN JIAOTONG UNIVERSITY   (China)

^d BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

metastasis; prostate cancer; xenografts

Indexed keywords

CELL MARKER; FIBRONECTIN; HYPOXIA INDUCIBLE FACTOR 1ALPHA; NERVE CELL ADHESION MOLECULE; TELOMERASE; UVOMORULIN; VASCULOTROPIN A; VIMENTIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CANCER CELL CULTURE; CANCER GROWTH; CARCINOGENESIS; CELL IMMORTALIZATION; CELL STRUCTURE; CHROMOSOME 15; CHROMOSOME 16Q; CHROMOSOME 18; CHROMOSOME 20; CHROMOSOME 21; CHROMOSOME 22; CHROMOSOME 3P; CHROMOSOME 7; CHROMOSOME 7Q; CHROMOSOME ANALYSIS; CHROMOSOME DELETION; CONTROLLED STUDY; CYTOGENETICS; ENZYME ACTIVITY; EPITHELIAL MESENCHYMAL TRANSITION; EPITHELIUM CELL; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; INOCULATION; KARYOTYPING; KIDNEY CAPSULE; MALE; METASTASIS; MOUSE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROSTATE CANCER; PROSTATE EPITHELIUM; PROTEIN ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TELOMERE; TUMOR XENOGRAFT; WESTERN BLOTTING;

ANIMALS; CELL LINE; CELL LINE, TRANSFORMED; CELL TRANSFORMATION, NEOPLASTIC; DNA, NEOPLASM; EPITHELIAL CELLS; EPITHELIAL-MESENCHYMAL TRANSITION; IMMUNOHISTOCHEMISTRY; KARYOTYPING; MALE; MICE; MICE, NUDE; POLYMERASE CHAIN REACTION; PROSTATE; PROSTATIC NEOPLASMS; TELOMERASE;

EID: 79952366631     PISSN: 02704137     EISSN: 10970045     Source Type: Journal    
DOI: 10.1002/pros.21278     Document Type: Article

References (29)

1. Jemal A, Siegel R, Ward E, Hao Y, Xu J, Thun MJ,. Cancer statistics, 2009. CA Cancer J Clin 2009; 59 (4): 225-249.
2. Stone KR, Mickey DD, Wunderli H, Mickey GH, Paulson DF,. Isolation of a human prostate carcinoma cell line (DU 145). Int J Cancer 1978; 21 (3): 274-281. (Pubitemid 8283045)
3. Kaighn ME, Narayan KS, Ohnuki Y, Lechner JF, Jones LW,. Establishment and characterization of a human prostatic carcinoma cell line (PC-3). Invest Urol 1979; 17 (1): 16-23. (Pubitemid 9239413)
4. van Bokhoven A, Varella-Garcia M, Korch C, Johannes WU, Smith EE, Miller HL, Nordeen SK, Miller GJ, Lucia MS,. Molecular characterization of human prostate carcinoma cell lines. Prostate 2003; 57 (3): 205-225. (Pubitemid 37267805)
5. Thiery JP, Acloque H, Huang RY, Nieto MA,. Epithelial-mesenchymal transitions in development and disease. Cell 2009; 139 (5): 871-890.
6. Micalizzi DS, Ford HL,. Epithelial-mesenchymal transition in development and cancer. Future Oncol 2009; 5 (8): 1129-1143.
7. Loric S, Paradis V, Gala JL, Berteau P, Bedossa P, Benoit G, Eschwege P,. Abnormal E-cadherin expression and prostate cell blood dissemination as markers of biological recurrence in cancer. Eur J Cancer 2001; 37 (12): 1475-1481. (Pubitemid 32744676)
8. De Wever O, Derycke L, Hendrix A, De Meerleer G, Godeau F, Depypere H, Bracke M,. Soluble cadherins as cancer biomarkers. Clin Exp Metastas 2007; 24 (8): 685-697. (Pubitemid 350159736)
9. Weijerman PC, Konig JJ, Wong ST, Niesters HG, Peehl DM,. Lipofection-mediated immortalization of human prostatic epithelial cells of normal and malignant origin using human papillomavirus type 18 DNA. Cancer Res 1994; 54 (21): 5579-5583. (Pubitemid 24346250)
10. Weijerman PC, van Drunen E, Konig JJ, Teubel W, Romijn JC, Schroder FH, Hagemeijer A,. Specific cytogenetic aberrations in two novel human prostatic cell lines immortalized by human papillomavirus type 18 DNA. Cancer Genet Cytogenet 1997; 99 (2): 108-115. (Pubitemid 27514980)
11. Herbert BS, Hochreiter AE, Wright WE, Shay JW,. Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol. Nature Protoc 2006; 1 (3): 1583-1590.
12. Webber MM, Bello D, Kleinman HK, Hoffman MP,. Acinar differentiation by non-malignant immortalized human prostatic epithelial cells and its loss by malignant cells. Carcinogenesis 1997; 18 (6): 1225-1231. (Pubitemid 28132514)
13. Lang SH, Smith J, Hyde C, Macintosh C, Stower M, Maitland NJ,. Differentiation of prostate epithelial cell cultures by Matrigel/stromal cell glandular reconstruction. In Vitro Cell Dev Biol Anim 2006; 42 (8-9): 273-280. (Pubitemid 44851885)
14. Xie D, Gore C, Liu J, Pong RC, Mason R, Hao G, Long M, Kabbani W, Yu L, Zhang H, Chen H, Sun X, Boothman DA, Min W, Hsieh JT,. Role of DAB2IP in modulating epithelial-to-mesenchymal transition and prostate cancer metastasis. Proc Natl Acad Sci USA 2010; 107 (6): 2485-2490.
15. Mak P, Leav I, Pursell B, Bae D, Yang X, Taglienti CA, Gouvin LM, Sharma VM, Mercurio AM,. ERbeta impedes prostate cancer EMT by destabilizing HIF-1alpha and inhibiting VEGF-mediated snail nuclear localization: Implications for Gleason grading. Cancer Cell 2010; 17 (4): 319-332.
16. Hurlin PJ, Kaur P, Smith PP, Perez-Reyes N, Blanton RA, McDougall JK,. Progression of human papillomavirus type 18-immortalized human keratinocytes to a malignant phenotype. Proc Natl Acad Sci USA 1991; 88 (2): 570-574. (Pubitemid 21915507)
17. Rhim JS, Webber MM, Bello D, Lee MS, Arnstein P, Chen LS, Jay G,. Stepwise immortalization and transformation of adult human prostate epithelial cells by a combination of HPV-18 and v-Ki-ras. Proc Natl Acad Sci USA 1994; 91 (25): 11874-11878. (Pubitemid 24368566)
18. Blanton RA, Perez-Reyes N, Merrick DT, McDougall JK,. Epithelial cells immortalized by human papillomaviruses have premalignant characteristics in organotypic culture. Am J Pathol 1991; 138 (3): 673-685.
19. Tyson DR, Inokuchi J, Tsunoda T, Lau A, Ornstein DK,. Culture requirements of prostatic epithelial cell lines for acinar morphogenesis and lumen formation in vitro: Role of extracellular calcium. Prostate 2007; 67 (15): 1601-1613. (Pubitemid 350036946)
20. Kleinman HK, Martin GR,. Matrigel: Basement membrane matrix with biological activity. Semin Cancer Biol 2005; 15 (5): 378-386. (Pubitemid 41140305)
21. Lin Y, Uemura H, Fujinami K, Hosaka M, Harada M, Kubota Y,. Telomerase activity in primary prostate cancer. J Urol 1997; 157 (3): 1161-1165. (Pubitemid 27066336)
22. Kallakury BV, Brien TP, Lowry CV, Muraca PJ, Fisher HA, Kaufman RP Jr, Ross JS,. Telomerase activity in human benign prostate tissue and prostatic adenocarcinomas. Diagn Mol Pathol 1997; 6 (4): 192-198. (Pubitemid 28047556)
23. Meeker AK, Hicks JL, Platz EA, March GE, Bennett CJ, Delannoy MJ, De Marzo AM,. Telomere shortening is an early somatic DNA alteration in human prostate tumorigenesis. Cancer Res 2002; 62 (22): 6405-6409. (Pubitemid 35364095)
24. Asai A, Oshima Y, Yamamoto Y, Uochi TA, Kusaka H, Akinaga S, Yamashita Y, Pongracz K, Pruzan R, Wunder E, Piatyszek M, Li S, Chin AC, Harley CB, Gryaznov S,. A novel telomerase template antagonist (GRN163) as a potential anticancer agent. Cancer Res 2003; 63 (14): 3931-3939. (Pubitemid 36917908)
25. Biroccio A, Leonetti C,. Telomerase as a new target for the treatment of hormone-refractory prostate cancer. Endocr Relat Cancer 2004; 11 (3): 407-421. (Pubitemid 39331274)
26. Shay JW, Wright WE,. Telomerase therapeutics for cancer: Challenges and new directions. Nat Rev Drug Discov 2006; 5 (7): 577-584. (Pubitemid 43971192)
27. Ray ME, Mehra R, Sandler HM, Daignault S, Shah RB,. E-cadherin protein expression predicts prostate cancer salvage radiotherapy outcomes. J Urology 2006; 176 (4): 1409-1414. (Pubitemid 44307202)
28. Tomita K, van Bokhoven A, van Leenders GJLH, Ruijter ETG, Jansen CFJ, Bussemakers MJG, Schalken JA,. Cadherin switching in human prostate cancer progression. Cancer Res 2000; 60 (13): 3650-3654. (Pubitemid 30482189)
29. Mani SA, Yang J, Brooks M, Schwaninger G, Zhou A, Miura N, Kutok JL, Hartwell K, Richardson AL, Weinberg RA,. Mesenchyme Forkhead 1 (FOXC2) plays a key role in metastasis and is associated with aggressive basal-like breast cancers. Proc Natl Acad Sci USA 2007; 104 (24): 10069-10074. (Pubitemid 47175264)