Performance of a non-grafted monolithic support for purification of supercoiled plasmid DNA

Journal of Chromatography A

Volumn 1218, Issue 13, 2011, Pages 1701-1706

  Sousa, A ^a   Bicho, D ^a   Tomaz, C T ^a   Sousa, F ^a   Queiroz, J A ^a  

^a UNIVERSITY OF BEIRA INTERIOR   (Portugal)

Author keywords

Affinity chromatography; Binding capacity; Breakthrough curves; CDI monolithic column; Supercoiled plasmid DNA.

Indexed keywords

BINDING CAPACITIES; BREAKTHROUGH CURVES; BREAKTHROUGH EXPERIMENT; CARBONYLDIIMIDAZOLE; CHROMATOGRAPHIC METHODS; CLINICAL APPLICATION; COMPACT STRUCTURES; ISOFORMS; LINEAR VELOCITY; MONOLITHIC COLUMNS; MONOLITHIC SUPPORTS; PLASMID DNA; PLASMID DNA (PDNA); PLASMID PURIFICATION; RATE DEPENDENCE; SPECIFIC RECOGNITION; SUPERCOILED; TRANSFER PROPERTIES;

AFFINITY CHROMATOGRAPHY; BIOMOLECULES; CHROMATOGRAPHIC ANALYSIS; DNA; FUNCTIONAL GROUPS; GRAFTING (CHEMICAL); NUCLEIC ACIDS; PURIFICATION;

COLUMN CHROMATOGRAPHY;

AMMONIUM SULFATE; CARBONYLDILMIDAZOLE; PLASMID DNA; PYRROLE DERIVATIVE; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; BINDING SITE; CONTROLLED STUDY; DNA BINDING; DNA CONFORMATION; DNA DEGRADATION; DNA PURIFICATION; DNA STRUCTURE; ESCHERICHIA COLI; FLOW RATE; GEL ELECTROPHORESIS; HYDROPHOBICITY; IONIC STRENGTH; MATHEMATICAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL;

AMMONIUM SULFATE; CHROMATOGRAPHY, AFFINITY; DNA, SUPERHELICAL; HYDROGEN-ION CONCENTRATION; MODELS, CHEMICAL; PHYSICOCHEMICAL PROCESSES; PLASMIDS;

EID: 79952363162     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2010.12.018     Document Type: Article

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