메뉴 건너뛰기




Volumn 21, Issue 6, 2011, Pages 1786-1791

Anti-obesity compounds in green leaves of Eucommia ulmoides

Author keywords

Anti obesity; Asperuloside; Chronic high dose administration; Eucommia ulmoides Oliv.; Iridoid

Indexed keywords

ASPERULOSIDE; CHLOROGENIC ACID; CHOLESTEROL; EUCOMMIA ULMOIDES EXTRACT; FATTY ACID; GENIPOSIDIC ACID; PLANT MEDICINAL PRODUCT; POLYSTYRENE; TRIACYLGLYCEROL; UNCLASSIFIED DRUG;

EID: 79952360200     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2011.01.060     Document Type: Article
Times cited : (102)

References (27)
  • 5
    • 0003658329 scopus 로고
    • Jiangsu New Medical College, ed. Shanghai Science and Technology Publishing House: Shanghai
    • Chinese Materia Medica Dictionary, Jiangsu New Medical College, ed. Shanghai Science and Technology Publishing House: Shanghai; 1977, p 1031.
    • (1977) Chinese Materia Medica Dictionary , pp. 1031
  • 16
    • 79952362128 scopus 로고    scopus 로고
    • 2O fractions (Fr. 1; 4.52 kg), 30% MeOH fractions (Fr. 2; 432 g), 50% MeOH fractions (Fr. 3; 215 g), 80% MeOH fractions (Fr. 4; 76.7 g) and 100% MeOH fractions (Fr. 5; 2.4 g), in the order of elution
    • 2O fractions (Fr. 1; 4.52 kg), 30% MeOH fractions (Fr. 2; 432 g), 50% MeOH fractions (Fr. 3; 215 g), 80% MeOH fractions (Fr. 4; 76.7 g) and 100% MeOH fractions (Fr. 5; 2.4 g), in the order of elution.
  • 17
    • 79952362962 scopus 로고    scopus 로고
    • Female ICR mice (4 weeks old; 23-28 g) purchased from SLC, Inc. (Shizuoka, Japan) were maintained at a temperature of 23-26 °C, relative humidity of 40-60%, and a 12-h light-dark cycle (lights on at 8 AM and off at 8 PM) for 1 week after arrival. Mice were divided into groups (n = 8) based on body weight. The experimental diet consisted of 40% beef tallow, 36% casein, 9% granulated sugar, 4% mineral mixture, 1% vitamin mixture, 10% cornstarch, and test materials (beef tallow, casein, granulated sugar, mineral mixture, vitamin mixture, cornstarch; Oriental Yeast Co., Ltd, Tokyo, Japan). The high-fat diet (HFD) group received feed in which one of the test materials was replaced by 10% casein
    • Female ICR mice (4 weeks old; 23-28 g) purchased from SLC, Inc. (Shizuoka, Japan) were maintained at a temperature of 23-26 °C, relative humidity of 40-60%, and a 12-h light-dark cycle (lights on at 8 AM and off at 8 PM) for 1 week after arrival. Mice were divided into groups (n = 8) based on body weight. The experimental diet consisted of 40% beef tallow, 36% casein, 9% granulated sugar, 4% mineral mixture, 1% vitamin mixture, 10% cornstarch, and test materials (beef tallow, casein, granulated sugar, mineral mixture, vitamin mixture, cornstarch; Oriental Yeast Co., Ltd, Tokyo, Japan). The high-fat diet (HFD) group received feed in which one of the test materials was replaced by 10% casein.
  • 18
    • 79952360659 scopus 로고    scopus 로고
    • For determination of the major components (geniposidic acid, asperuloside and chlorogenic acid) in the samples, an HPLC system comprised of a YMC-Pack ODS column (YMC Co., Ltd, Kyoto, Japan; 6.0 × 150 mm, 5 μm), LC-10AT pumps, and a SPD-10A UV detector (Shimazu Co., Ltd, Kyoto, Japan) was assembled. The mobile phase was methanol/water/phosphoric acid (870:130:1, v/v) at a flow rate of 1.0 ml/min. The detection wavelength was 215 nm
    • For determination of the major components (geniposidic acid, asperuloside and chlorogenic acid) in the samples, an HPLC system comprised of a YMC-Pack ODS column (YMC Co., Ltd, Kyoto, Japan; 6.0 × 150 mm, 5 μm), LC-10AT pumps, and a SPD-10A UV detector (Shimazu Co., Ltd, Kyoto, Japan) was assembled. The mobile phase was methanol/water/phosphoric acid (870:130:1, v/v) at a flow rate of 1.0 ml/min. The detection wavelength was 215 nm.
  • 19
    • 79952362294 scopus 로고    scopus 로고
    • Test materials were prepared by adding 10% EGLP, 7.97% Fr. 1, 0.76% Fr. 2, 0.38% Fr. 3, 0.14% Fr. 4 and 0.004% Fr. 5
    • Test materials were prepared by adding 10% EGLP, 7.97% Fr. 1, 0.76% Fr. 2, 0.38% Fr. 3, 0.14% Fr. 4 and 0.004% Fr. 5.
  • 20
    • 79952363826 scopus 로고    scopus 로고
    • note
    • After 4 weeks of administration, body weight was measured and mice were sacrificed by decapitation without loading stress. Blood was collected immediately after the animals had been sacrificed by decapitation. The blood was centrifuged (3000 rpm, 30 min), and the separated plasma was stored at -80 °C until measurement. The levels of plasma TG and plasma total cholesterol were measured using commercially available enzyme kits (triglyceride: E-Test Wako, cholesterol: E-Test Wako; Wako Pure Chemicals Industries, Ltd, Osaka, Japan). Levels of FFA were measured using commercially available enzyme kits (FFA kit: Wako Pure Chemicals Industries, Ltd, Osaka, Japan). White adipose tissues (WAT) (abdominal visceral fat) were immediately removed. All procedures were carried out in accordance with the Guidelines for Animal Experiments at Kobayashi Pharmaceutical Co., Ltd, R & D Center, the Japanese Government Animal Protection and Management Law (No. 105), and the Japanese Government Notification on Feeding and Safekeeping of Animals (No. 6).
  • 21
    • 79952364258 scopus 로고    scopus 로고
    • note
    • Differences between groups were assessed with the unpaired Tukey t-test using a statistical package from the spss13.0 program. Differences were considered significant at p <0.05.
  • 25
    • 79952364090 scopus 로고    scopus 로고
    • 2O-MeOH (1:1-6:1) and then purified by Daisogel SP-120-40/60-ODS-B (100 × 1000 mm) eluted with 80% MeOH, vacuum-dried and freeze-dried, and washed with acetone to furnish asperuloside (31.3 g, purity 99.5%). Geniposidic acid and asperuloside were extracted and isolated from EGLP
    • 2O-MeOH (1:1-6:1) and then purified by Daisogel SP-120-40/60-ODS-B (100 × 1000 mm) eluted with 80% MeOH, vacuum-dried and freeze-dried, and washed with acetone to furnish asperuloside (31.3 g, purity 99.5%). Geniposidic acid and asperuloside were extracted and isolated from EGLP.
  • 26
    • 79952364506 scopus 로고    scopus 로고
    • Test materials were prepared by adding 0.63% geniposidic acid, 0.45% asperuloside, and 0.44% chlorogenic acid
    • Test materials were prepared by adding 0.63% geniposidic acid, 0.45% asperuloside, and 0.44% chlorogenic acid.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.