Telomere alterations and genomic instability in long-term cultures of normal human fibroblasts irradiated with X rays and protons

Radiation Protection Dosimetry

Volumn 143, Issue 2-4, 2011, Pages 274-278

  Berardinelli, F ^a,b   Antoccia, A ^a,b   Cherubini, R ^c   de Nadal, V ^c   Gerardi, S ^c   Tanzarella, C ^a,b   Sgura, A ^a,b  

^a ROMA TRE UNIVERSITY   (Italy)

^b INFN SEZIONE DI ROMA   (Italy)

^c LABORATORI NAZIONALI DI LEGNARO   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL LINE; CHROMOSOMAL INSTABILITY; GENETICS; HUMAN; PHOTON; RADIATION DOSE; RADIATION EXPOSURE; RADIATION RESPONSE; TELOMERE; ULTRASTRUCTURE; X RAY;

CELL LINE; CHROMOSOMAL INSTABILITY; DOSE-RESPONSE RELATIONSHIP, RADIATION; HUMANS; PHOTONS; RADIATION DOSAGE; TELOMERE; X-RAYS;

EID: 79952349383     PISSN: 01448420     EISSN: 17423406     Source Type: Journal    
DOI: 10.1093/rpd/ncq486     Document Type: Article

References (29)

1. Goodhead, D. T., Thacker, J. and Cox, R. Effects of radiations of different qualities on cells: molecular mechanisms of damage and repair. Int. J. Radiat. Biol. 63, 543-556 (1993).
2. Ward, J. F. The complexity of DNA damage: relevance to biological consequences. Int. J. Radiat. Biol. 66, 427-432 (1994).
3. de Lange, T. Protection of mammalian telomeres. Oncogene 21, 532-540 (2002).
4. Bailey, S. M. and Murnane, J. P. Telomeres, chromosome instability and cancer. Nucl. Acids Res. 34, 2408-2417 (2006).
5. Littlefield, L. G., Colyer, S. P., Joiner, E. E. and DuFrain, R. J. Sister chromatid exchanges in human lymphocytes exposed to ionizing radiation during G0. Radiat. Res. 78, 514-521 (1979).
6. Morgan, W. F. and Crossen, P. E. X-irradiation and sister chromatid exchanges in cultured human lymphocytes. Environ. Mutat. 2, 149-155 (1980).
7. Savage, J. R. K. and Holloway, M. Induction of SCE by d (42 MeV)-Be neutrons in unstimulated humanblood lymphocytes. Int. J. Radiat. Res. 52, 483 (1987).
8. Savage, J. R. K. and Holloway, M. Induction of sisterchromatid exchanges by d (42 MeV)-Be neutrons in unstimulated human blood lymphocytes. Br. J. Radiol. 61, 231-234 (1988).
9. Aghamohammadi, S. Z., Goodhead, D. T. and Savage, J. R. K. Induction of sister chromatid exchanges (SCE) in G0 lymphocytes by plutonium-238 a-particles. Int. J. Radiat. Biol. 53, 909-915 (1988).
10. Awa, A. A., Sofuni, T., Honda, T., Itoh, M., Neriishi, S. and Otake, M. Relationship between the radiation dose and chromosome aberrations in atomic bomb survivors of Hiroshima and Nagasaki. J. Radiat. Res. (Tokyo). 19, 126-140 (1978).
11. Edwards, A. A. et al. Review of translocations detected by FISH for retrospective biological dosimetry applications. Radiat. Prot. Dosimetry. 113, 396-402 (2005).
12. Tucker, J. D. FISH cytogenetics and the future of radiation biodosimetry. Radiat. Prot. Dosim. 97, 55-60 (2001).
13. Boei, J. J., Vermeulen, S. and Natarajan, A. T. Dose-response curves for X-ray induced interchanges and interarm intrachanges in human lymphocytes using armspecific probes for chromosome 1. Mutat. Res. 404, 45-53 (1998).
14. van Buul, P. P. W., Zandman, I. M., Natarajan, A. T. and Boei, J. W. A. Non-random chromosomal involvement in radiation-induced translocations in mouse spermatogonial stem cells. Mutagenesis 11, 391-393 (1996).
15. Boei, J. J. W. A., Balajee, A. S., de Boer, P., Rens, W., Aten, J.A., Mullenders, L. H. F. and Natarajan, A.T. Construction of mouse chromosome-specific DNA libraries and their use for the detection of X-ray-induced aberrations. Int. J. Radiat. Biol. 65, 583-590 (1994).
16. Hande, M.P., Boei, J. J. W. A., Granath, F. and Natarajan, A. T. Induction and persistence of cytogenetic damage in mouse splenocytes following whole-body X-irradiation analysed by fluorescence in situ hybridization. I. Dicentrics and translocations. Int. J. Radiat. Biol. 69, 437-446 (1996).
17. Spruill, M. D., Ramsey, M. J., Swiger, R. R., Nath, J. and Tucker, J. D. The persistence of aberrations in mice induced by gamma radiation as measured by chromosome painting. Mutat. Res. 356, 135-145 (1996).
18. Tucker, J. D., Sorensen, K. J., Chu, C. S., Nelson, D. O., Ramsey, M. J., Urlando, C. and Heddle, J. A. The accumulation of chromosome aberrations and Dlb-1 mutations in mice with highly fractionated exposure to gamma radiation. Mutat. Res. 400, 321-335 (1998).
19. Sorensen, K. J., Abramsson Zetterberg, L., Nelson, D. O., Grawe, J. and Tucker, J. D. The in vivo dose rate effect of chronic gamma radiation in mice: translocation and micronucleus analyses. Mutat. Res. 457, 125-136 (2000).
20. Grigorova, M., Boei, J. J. W. A., van Duyn-Goedhart, A., Natarajan, A. T. and van Buul, P. P. X-ray induced translocations in bone marrow cells of SCID and wild type mice detected by fluorescence in situ hybridization using chromosome specific DNA libraries. Mutat. Res. 331, 39-45 (1995).
21. van Buul, P. P. W., Zandman, I. M., Grigorova, M., Boei, J. J. W. A. and Natarajan, A. T. Mitotic and meiotic detection of radiation-induced translocations in mouse stem cell spermatogonia using fluorescence in situ hybridization. Chromosome Res. 3, 427-432 (1995).
22. Belli, M., Cherubini, R., Galeazzi, G., Mazzucato, S., Moschini, G., Sapora, O., Simone, G. and Tabacchini, M. A. Proton irradiation facility for radiobiological studies at a 7 MV Van de Graaff accelerator. Nucl. Instr. and Meth. A. 256, 576-580 (1987).
23. Durante, M., Furusawa, Y. and Gotoh, E. A simple method for simultaneous interphase-metaphase chromosome analysis in biodosimetry. Int. J. Radiat. Biol. 74, 457-462 (1998).
24. Ferlicot, S., Youssef, N., Feneux, D., Delhommeau, F., Paradis, V. and Bedossa, P. Measurement of telomere length on tissue sections using quantitative fluorescence in situ hybridization (Q-FISH). J. Pathol. 200, 661-666 (2003).
25. Perner, S. et al. Quantifying telomere lengths of human individual chromosome arms by centromere-calibrated fluorescence in situ hybridization and digital imaging. Am. J. Pathol. 163, 1751-1756 (2003).
26. Sgura, A., Antoccia, A., Berardinelli, F., Cherubini, R., Gerardi, S., Zilio, C. and Tanzarella, C. Telomere length in mammalian cells exposed to low- and high-LET radiations. Radiat. Prot. Dosimetry. 122, 176-179 (2006).
27. Berardinelli, F., Antoccia, A., Cherubini, R., De Nadal, V., Gerardi, S., Cirrone, G. A. P., Tanzarella, C. and Sgura, A. Transient activation of the ALT pathway in human primary fibroblasts exposed to high-LET radiation. Radiat. Res. (2010). Epub ahead of print.
28. Wilson, D. M. III and Thompson, L. H. Molecular mechanisms of sister-chromatid exchange. Mutat. Res. 616, 11-23 (2007).
29. Kadhim, M. A., Hill, M. A. and Moore, S. R. Genomic instability and the role of radiation quality. Radiat. Prot. Dosimetry. 122, 221-227 (2006).