The spectrum of cytokeratins expressed in the adult human cornea, limbus and perilimbal conjunctiva

Histology and Histopathology

Volumn 26, Issue 3, 2011, Pages 323-331

  Merjava, Stanislava ^a   Neuwirth, Ales ^b   Tanzerova, Michaela ^a   Jirsova, Katerina ^a  

^a CHARLES UNIVERSITY   (Czech Republic)

^b INSTITUTE OF MOLECULAR GENETICS   (Czech Republic)

Author keywords

Cornea; Cytokeratins; Epithelium; Immunohistochemistry

Indexed keywords

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; KERATIN; RNA;

ADULT; AGED; ARTICLE; BIOSYNTHESIS; CONJUNCTIVA; CORNEA; CORNEA EPITHELIUM; CORNEA LIMBUS; FEMALE; FLUORESCENCE MICROSCOPY; FLUORESCENT ANTIBODY TECHNIQUE; GENETICS; HUMAN; IMMUNOHISTOCHEMISTRY; MALE; METABOLISM; MIDDLE AGED; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCLERA;

ADULT; AGED; CONJUNCTIVA; CORNEA; EPITHELIUM, CORNEAL; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HUMANS; IMMUNOHISTOCHEMISTRY; KERATINS; LIMBUS CORNEAE; MALE; MICROSCOPY, FLUORESCENCE; MIDDLE AGED; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; SCLERA;

EID: 79952320849     PISSN: 02133911     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (60)

1. Bowden P.E., Haley J.L., Kansky A., Rothnagel J.A., Jones D.O. and Turner R.J. (1995). Mutation of a type II keratin gene (K6a) in pachyonychia congenita. Nat. Genet. 10, 363-365.
2. Caulin C., Ware C.F., Magin T.M. and Oshima R.G. (2000). Keratin-dependent, epithelial resistance to tumor necrosis factor-induced apoptosis. J. Cell Biol. 149, 17-22. (Pubitemid 30196695)
3. Chang C.Y., Green C.R., McGhee C.N. and Sherwin T. (2008). Acute wound healing in the human central corneal epithelium appears to be independent of limbal stem cell influence. Invest. Ophthalmol. Vis. Sci. 49, 5279-5286.
4. Chen Z., de Paiva C.S., Luo L., Kretzer F.L., Pflugfelder S.C. and Li D.Q. (2004). Characterization of putative stem cell phenotype in human limbal epithelia. Stem Cells 22, 355-366. (Pubitemid 38736619)
5. Chipev C.C., Korge B.P., Markova N., Bale S.J., DiGiovanna J.J., Compton J.G. and Steinert P.M. (1992). A leucine-proline mutation in the H1 subdomain of keratin 1 causes epidermolytic hyperkeratosis. Cell 70, 821-828.
6. Clausen H., Vedtofte P., Moe D., Dablesteen E., Sun T.T. and Dale B. (1986). Differentiation-dependent expression of keratins in human oral epithelia. J. Invest. Dermatol. 86, 249-254. (Pubitemid 16124157)
7. Cockerham G.C., Laver N.V., Hidayat A.A. and McCoy D.L. (2002). An immunohistochemical anylysis and comparison of posterior polymorphous dystrophy with congenital hereditary endothelial dystrophy. Cornea 21, 787-791. (Pubitemid 35239502)
8. Coulombe P.A., Hutton M.E., Letai A., Hebert A., Paller A.S. and Fuchs E. (1991). Point mutation in human keratin 14 genes of epidermolysis bullosa simplex patients: genetic and functional analyses. Cell 66, 1301-1311. (Pubitemid 121001418)
9. Davanger M. and Evensen A. (1971). Role of the pericorneal structure in renewal of corneal epithelium. Nature 229, 560-561.
10. Di Iorio E., Barbaro V., Ruzza A., Ponzin D., Pellegrini G. and De Luca M. (2005). Isoforms of DeltaNp63 and the migration of ocular limbal cells in human corneal regeneration. Proc. Natl. Acad. Sci. USA 102, 9523-9528. (Pubitemid 40981714)
11. Donisi P.M., Rama P., Fasolo A. and Ponzin D. (2003). Analysis of limbal stem cell deficiency by corneal impression cytology. Cornea 22, 533-538. (Pubitemid 36909732)
12. Elder M.J., Hiscott P. and Dart J.K. (1997). Intermediate filament expression by normal and diseased human corneal epithelium. Hum. Pathol. 28, 1348-1354. (Pubitemid 28013628)
13. Figueira E.C., Di Girolamo N., Coroneo M.T. and Wakefield D. (2007). The phenotype of limbal epithelial stem cells. Invest. Ophthalmol. Vis. Sci. 48, 144-156. (Pubitemid 47251397)
14. Franssen M.E., Boezeman J.B., Van De Kerkhof P.C. and Van Erp P.E. (2004). Monitoring hyperproliferative disorders in human skin: flow cytometry of changing cytokeratin expression. Cytometry B. Clin. Cytom. 57, 32-39. (Pubitemid 38297621)
15. Hanna C., Bicknell D.S. and O'Brien J.E. (1961). Cell turnover in the adult human eye. Arch. Ophthalmol. 65, 695-698.
16. Irvine A.D., Corden L.D., Swensson O., Swensson B., Moore J.E., Frazer D.G., Smith F.J., Knowlton R.G., Christophers E., Rochels R., Uitto J. and McLean W.H. (1997). Mutations in cornea-specific keratin K3 or K12 genes cause Meesmann's corneal dystrophy. Nat. Genet. 16, 184-187. (Pubitemid 27240618)
17. Kasper M., Moll R., Stosiek P. and Karsten U. (1988). Patterns of cytokeratin and vimentin expression in the human eye. Histochemistry 89, 369-377.
18. Kasper M., Stosiek P. and Lane B. (1992). Cytokeratin and vimentin heterogeneity in human cornea. Acta Histochem. 93, 371-381. (Pubitemid 23008293)
19. Kim S., Wong P. and Coulombe P.A. (2006). A keratin cytoskeletal protein regulates protein synthesis and epithelial cell growth. Nature 441, 362-365. (Pubitemid 44050202)
20. Kivelä T. and Uusitalo M. (1998). Structure, development and function of cytoskeletal elements in non-neuronal cells of the human eye. Prog. Retin. Eye Res. 17, 385-428. (Pubitemid 28342388)
21. Krenzer K.L. and Freddo T.F. (1997). Cytokeratin expression in normal human bulbar conjunctiva obtained by impression cytology. Invest. Ophthalmol. Vis. Sci. 38, 142-152. (Pubitemid 27051533)
22. Kurpakus M.A., Stock E.L. and Jones J.C. (1990). Expression of the 55-kD/64-kD corneal keratins in ocular surface epithelium. Invest. Ophthalmol. Vis. Sci. 31, 448-456. (Pubitemid 20117059)
23. Lane E.B., Rugg E.L., Navsaria H., Leigh I.M., Heagerty A.H., Ishida-Yamamoto A. and Eady R.A. (1992). A mutation in the conserved helix termination peptide of keratin 5 in hereditary skin blistering. Nature 356, 244-246.
24. Lauweryns B., van den Oord J.J., De Vos R. and Missotten L. (1993a). A new epithelial cell type in the human cornea. Invest. Ophthalmol. Vis. Sci. 34, 1983-1990. (Pubitemid 23148634)
25. Lauweryns B., van den Oord J.J. and Missotten L. (1993b). The transitional zone between limbus and peripheral cornea. An immunohistochemical study. Invest. Ophthalmol. Vis. Sci. 34, 1991-1999. (Pubitemid 23148635)
26. Leube R.E., Bosch F.X., Romano V., Zimbelmann R., Höfler H. and Franke W.W. (1986). Cytokeratin expression in simple epithelia. III. Detection of mRNAs encoding human cytokeratins nos. 8 and 18 in normal and tumor cells by hybridization with cDNA sequences in vitro and in situ. Differentiation 33, 69-85. (Pubitemid 17053564)
27. Lin A.N., Murphy F., Brodie S.E. and Carter D.M. (1994). Review of ophthalmic findings in 204 patients with epidermolysis bullosa. Am. J. Ophthalmol. 118, 384-390. (Pubitemid 24281733)
28. Liu Y., Lyle S., Yang Z. and Cotsarelis G. (2003). Keratin 15 promoter targets putative epithelial stem cells in the hair follicle bulge. J. Invest. Dermatol. 121, 963-968. (Pubitemid 37430960)
29. Lloyd C., Yu Q.C., Cheng J., Turksen K., Degenstein L., Hutton E. and Fuchs E. (1995). The basal keratin network of stratified squamous epithelia: defining K15 function in the absence of K14. J. Cell Biol. 129, 1329-1344.
30. Lyle S., Christofidou-Solomidou M., Liu Y., Elder D.E., Albelda S. and Cotsarelis G. (1998). The C8/144b monoclonal antibody recognizes cytokeratin 15 and defines the location of human hair follicle stem cells. J. Cell Sci. 111, 3179-3188. (Pubitemid 28532428)
31. Lyngholm M., Hoyer P.E., Vorum H., Nielsen K., Ehlers N. and Mollgard K. (2008). Immunohistochemical markers for corneal stem cells in the early developing human eye. Exp. Eye Res. 87, 115-121.
32. Mansbridge J.N. and Knapp A.M. (1987). Changes in keratinocyte maturation during wound healing. J. Invest. Dermatol. 89, 253-263. (Pubitemid 17135369)
33. McLean W.H., Eady R.A., Dopping-Hepenstal P.J., McMillan J.R., Leigh I.M., Navsaria H.A., Higgins C., Harper J.I., Paige D.G., Morley S.M. and Lane E.B. (1994). Mutations in the rod 1A domain of keratins 1 and 10 in bullous congenital ichthyosiform erythroderma (BCIE). J. Invest. Dermatol. 102, 24-30. (Pubitemid 24039558)
34. McLean W.H., Rugg E.L., Lunny D.P., Morley S.M., Lane E.B., Swensson O., Dopping-Hepenstal P.J., Griffiths W.A., Eady R.A., Higgins C., Navsaria H.A., Leigh I.M., Strachen T., Kunkeler l. and Munro C.S. (1995). Keratin 16 and keratin 17 mutations cause pachyonychia congenita. Nat. Genet. 9, 273-278.
35. Merjava S., Neuwirth A., Mandys V. and Jirsova K. (2009). Cytokeratins 8 and 18 in adult human corneal endothelium. Exp. Eye Res. 89, 426-431.
36. Moll R., Franke W.W., Schiller D.L., Geiger B. and Krepler R. (1982). The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11-24. (Pubitemid 13215192)
37. Moll R., Divo M. and Langbein L. (2008). The human keratins: biology and pathology. Histochem. Cell Biol. 129, 705-733.
38. Morgan P.R., Shirlaw P.J., Johnson N.W., Leigh I.M. and Lane E.B. (1987). Potential applications of anti-keratin antibodies in oral diagnosis. J. Oral Pathol. 16, 212-222.
39. Mygind H., Nielsen B., Moe D., Clausen H., Dabelsteen E. and Clausen P.P. (1988). Antikeratin antibodies in routine diagnostic pathology. A comparison of 10 different commercial antikeratins. APMIS 96, 1009-1022. (Pubitemid 19007389)
40. Nelson J.D. and Cameron J.D. (2005). Cornea. In: Cornea. 2nd edition. Krachmer J.H., Mannis M.J. and Holland E.J. (eds). Elsevier MOSBY Inc. pp 3-26.
41. Olsen T. (1986). On the calculation of power from curvature of the cornea. Br. J. Ophthalmol. 70, 152-154. (Pubitemid 16128001)
42. Owens D.W. and Lane E.B. (2004). Keratin mutations and intestinal pathology. J. Pathol. 204, 377-385. (Pubitemid 39468149)
43. Oshima R.G., Baribault H. and Caulin C. (1996). Oncogenic regulation and function of keratins 8 and 18. Cancer Metastasis Rev. 15, 445-471. (Pubitemid 27067165)
44. Pfaffl M.W. (2001). A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29, e45.
45. Pitz S. and Moll R. (2002). Intermediate-filament expression in ocular tissue. Prog. Retin. Eye Res. 21, 241-262. (Pubitemid 34607438)
46. Ramaekers F., Huysmans A., Schaart G., Moesker O. and Vooijs P. (1987). Tissue distribution of keratin 7 as monitored by a monoclonal antibody. Exp. Cell. Res. 170, 235-249. (Pubitemid 17089760)
47. Richard G., De Laurenzi V., Didona B., Bale S.J. and Compton J.G. (1995). Keratin 13 point mutation underlies the hereditary mucosal epithelial disorder white sponge nevus. Nat. Genet. 11, 453-455.
48. Ross J.R., Foulks G.N., Sanfilippo F.P. and Howell D.N. (1995). Immunohistochemical analysis of the pathogenesis of posterior polymorphous dstrophy. Arch. Ophthalmol. 113, 340-345.
49. Rothnagel J.A., Dominey A.M., Dempsey L.D., Longley M.A., Greenhalgh D.A., Gagne T.A., Huber M., Frenk E., Hohl D. and Roop D.R. (1992). Mutations in the rod domains of keratins 1 and 10 in epidermolytic hyperkeratosis. Science 257, 1128-1130.
50. Rugg E.L., McLean W.H., Allison W.E., Lunny D.P., Macleod R.I., Felix D.H., Lane E.B. and Munro C.S. (1995). A mutation in the mucosal keratin K4 is associated with oral white sponge nevus. Nat. Genet. 11, 450-452.
51. Sack R.A., Nunes I., Beaton A. and Morris C. (2001). Host-defense mechanism of the ocular surfaces. Biosci. Rep. 21, 463-480. (Pubitemid 34211830)
52. Sawaf M.H., Ouhayoun J.P. and Forest N. (1991). Cytokeratin profiles in oral epithelia: a review and new classification. J. Biol. Buccale 19, 187-198.
53. Schlötzer-Schrehardt U. and Kruse F.E. (2005). Identification and characterization of limbal stem cells. Exp. Eye Res. 81, 247-264. (Pubitemid 41208160)
54. Sun T.T., Eichner R., Schermer A., Cooper D., Nelson W.G. and Weiss R.A. (1984). The transformed phenotype. In: The cancer cell. Vol. 1. Levine A., Topp W., van de Woude G. and Watson J.D. (eds). Cold Spring Harbor Laboratory. Cold Spring Harbor, NY. pp 169-176.
55. Thoft R.A. and Friend J. (1983). The X, Y, Z hypothesis of corneal epithelial maintenance. Invest. Ophthalmol. Vis. Sci. 24, 1442-1443.
56. Tseng S.C. (1989). Concept and application of limbal stem cells. Eye 3, 141-157. (Pubitemid 20018556)
57. Upasani O.S., Vaidya M.M. and Bhisey A.N. (2004). Database on monoclonal antibodies to cytokeratins. Oral Oncol. 40, 236-256. (Pubitemid 38121561)
58. van der Velden L.A., Manni J.J., Ramaekers F.C. and Kuijpers W. (1999). Expression of intermediate filament proteins in benign lesions of the oral mucosa. Eur. Arch. Otorhinolaryngol. 256, 514-519.
59. Yoshida S., Shimmura S., Kawakita T., Miyashita H., Den S., Shimazaki J. and Tsubota K. (2006). Cytokeratin 15 can be used to identify the limbal phenotype in normal and diseased ocular surfaces. Invest. Ophthalmol. Vis. Sci. 47, 4780-4786. (Pubitemid 46586375)
60. Zatloukal K., Stumptner C., Lehner M., Denk H., Baribault H., Eshkind L.G. and Franke W.W. (2000). Cytokeratin 8 protects from hepatotoxicity, and ist ratio to cytokeratin 18 determines the ability of hepatocytes to form Mallory bodies. Am. J. Pathol. 156, 1263-1274. (Pubitemid 30660005)