Multiplex polymerase chain reaction (PCR) assay for simultaneous detection of shiga-like toxin (stx1 and stx2), intimin (eae) and invasive plasmid antigen H (ipaH) genes in diarrheagenic Escherichia coli

African Journal of Biotechnology

Volumn 10, Issue 9, 2011, Pages 1522-1526

  Sharifi Yazdi, M K ^a   Akbari, A ^a   Soltan Dallal, M M ^a  

^a TEHRAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

Diarrheagenic Escherichia coli; Multiplex polymerase chain reaction (PCR); Shiga like toxin

Indexed keywords

BACTERIAL ANTIGEN; BACTERIAL DNA; INTIMIN; INVASIVE PLASMID ANTIGEN H; UNCLASSIFIED DRUG; VEROTOXIN 1; VEROTOXIN 2;

ARTICLE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CONTROLLED STUDY; DIARRHEA; DIARRHEAGENIC ESCHERICHIA COLI; ENTEROHEMORRHAGIC ESCHERICHIA COLI; ENTEROINVASIVE ESCHERICHIA COLI; ENTEROPATHOGENIC ESCHERICHIA COLI; FECES ANALYSIS; GENE IDENTIFICATION; GENE TARGETING; IRAN; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; SEROTYPING; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; TOXIN ANALYSIS;

ESCHERICHIA COLI;

EID: 79952304233     PISSN: 16845315     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (33)

1. Agin TS, Cantey JR, Boedeker EC, Wolf MK (1996). Characterization of the eae gene from rabbit enteropathogenic Escherichia coli strain RDEC-1 and comparison to other eae genes from bacteria that cause attaching-effacing lesions. FEMS Microbiol. Lett. 144: 249-258.
2. Akinjogunl OJ, Eghafona NO, Ekoi OH (2009). Diarrheagenic Escherichia coli (DEC): prevalence among in and ambulatory patients and susceptibility to antimicrobial chemotherapeutic agents. J. Bacteriol. Res.,1(3): 34-38.
3. Alikhani Y, Mirsalehian A, Aslani M (2006). Detection of typical and atypical enteropathogenic Escherichia coli (EPEC) in Iranian children with and without diarrhoea. J. Med. Microbiol. 55: 1159-1163.
4. Boerlin P (1999). Evolution of virulence factors in Shiga-toxin-producing Escherichia coli. CMLS, Cell. Mol. Life Sci. 56: 735-741.
5. Estelle L, Monique K, Lothar B (2006). Characterization of Shiga Toxin Gene (stx)-Positive and Intimin Gene (eae)-Positive Escherichia coli Isolates from Wastewater of Slaughterhouses in France. Appl. Environ. Microbiol. 72: 3245-3251.
6. Franzolin MR, Barbosa Alves, Rogéria Keller RC, Tardelli Gomes TA, Beutin L, Lima Barreto M (2005). Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil. Mem Inst Oswaldo Cruz, Rio de Janeiro. 100(4): 359-363.
7. Galane PM, Le Roux M (2001). Molecular epidemiology of Escherichia coli isolated from Young South African children with diarrhoeal diseases. J. Health Popul. Nutr.,19(1):31-38.
8. Harris JR, Mariano J, Wells JG, Payne BJ, Donnell HD, Cohen ML (1985). Person-to-Person transmission in an outbreak of Enteroinvasive Escherichia-coli. Am. J. Epidemiol., 122(2):245-252.
9. Ibram S, Munteanu A, Stolica B, Enescu M, Savulescu C, Costinea R Popa MI (2007). An outbreak of gastroenteritis in a campsite in Romania, Eurosurveillance Weekly Release.
10. Kalantar E, Soheili F, Salimi H, Soltan Dallal MM (2011). Frequency, antimicrobial susceptibility and plasmid profiles of Escherichia coli pathotypes obtained from children with acute diarrhea. Jndishapur J. Microbiol., 4(1): 23-28.
11. Katia RS, Aranda SH, Fabbricotti UF-N, Isabel CAS (2007). Single multiplex assay to identifysimultaneously enteropathogenic, enteroaggregative, enterotoxigenic, enteroinvasive and Shiga toxinproducing Escherichia coli strains in Brazilian children. FEMS Microbiol. Lett. 267: 145-150.
12. Law D (1994). Adhesion and its role in the virulence of enteropathogenic E. coli Clin. Microbiol. Rev. 7: 152-173.
13. Maricel V, Eileen K, Claudia D, Rosanna L (2005). Single Multiplex PCR Assay To Identify Simultaneously the Six Categories of Diarrheagenic Escherichia coli Associated with Enteric Infections. J. Clin. Microbiol. 43: 5362-5365.
14. Mitchell B, Cohen J, Nataro P (2005). Prevalence of diarrheagenic Escherichia coli in acute childhood enteritis: A prospective controlled study. J. Pediatr. 146: 54-61.
15. Moyenuddin M, Wachsmuth K, Moseley SL, Bopp CA, Blake PA (1989). Serotype, antimicrobial resistance, and adherence properties of Escherichia coli strains associated with outbreaks of Diarrheal illness in children in the united states. J. Clin. Microbiol. 27(10): 2234-2239.
16. Nataro JP, Kaper JB (1998). Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11: 142-201.
17. Sunabe T, Honma Y (1998). Relationship between O-serogroup and Presence of pathogenic factor genes in Escherichia coli. Microbiol. Immunol. 42: 845-849.
18. Osek J (2001). Multiplex polymerase chain reaction assay for identification of enterotoxigenic Escherichia coli strains. J. Vet. Diagn. Investing. 13: 308-311.
19. Pass MA, Odedra R, Batt RM (2000). Multiplex PCRs for identification of Escherichia coli virulence genes. J. Clin. Microbiol. 38: 2001-2004.
20. Paton AW, Paton JC (2002). Direct detection and characterization of Shiga toxigenic Escherichia coli by multiplex PCR for stx1, stx2, eae, ehxA, and saa. J. Clin. Microbiol. 40: 271-274.
21. Rappelli P, Maddau G, Mannu F, Colombo MM, Fiori PL, Cappuccinelli P (2001). Development of a set of multiplex PCR assays for the Simultaneous identification of enterotoxigenic, enteropathogenic, enterohemorrhagic and enteroinvasive Escherichia coli. Microbiologica, 24: 77-83.
22. Ratchtrachenchai OA, Subpasu S, Ito K (1997). Investigation on enteroaggregative Escherichia coli infection by multiplex PCR. Bull. Dep. Med. Sci. 39: 211-220.
23. Reyes D, Vilchez S, Paniagua M, Colque-Navarro P, Weintraub A, Möllby R, Kühn I (2010). Diarrheagenic Escherichia coli Markers and Phenotypes among Fecal E. coli Isolates Collected from Nicaraguan Infants. J. Clin. Microbiol., 48(9):3395-3396.
24. Rich C, Alfidja A, Sirot J, Joly B, Forestier C (2001). Identification of human enterovirulent Escherichia coli strains by multiplex PCR. J. Clin. Lab. Anal. 15: 100-103.
25. Stacy-Phipps S, Mecca JJ, Weiss JB (1995). Multiplex PCR assay and simple Preparation method for stool specimens detects enterotoxigenic Escherichia coli DNA during course of infection. J. Clin. Microbiol. 33(5): 1054-1059.
26. Sahilah AM, Aishah HN, Noraida I, Ahmad Azuhairi A (2010). Detection of Shiga Toxin 1 and 2 (stx1 and stx2) Genes in Escherichia coli O157:H7 Isolated from Retail Beef in Malaysia by Multiplex Polymerase Chain Reaction (PCR). Sains Malaysiana 39(1): 57-63.
27. Scotland SM, Smith HR, Said B, Willshaw GA, Cheasty T, Rowe B (1991). Identification of enteropathogenic E. coli isolated in Britain as enteroggregative or as members of a subclass of attachingandeffacing E. coli not hybridizing with the EPEC adherence-factor probe. J. Med. Microbiol. 35: 278-283.
28. Scotland SM, Smith HR, Rowe B (1991). E. coli o128 strains from infants with diarrhea commonly show localized adhesion and positivity in the fluorescentactin staining test but do not hybridize with an enteropathogenic. E. coli adherence factor probe. Infect. Immun. 59: 1569-1571.
29. Smith HR, Scotland SM, Stokes N, Rowe B (1990). Examination of strains belonging to enteropathogenic E. coli serogroups for genes encoding EPEC adherence factor and vero cytotoxins. J. Med. Microbiol. 31: 235-240.
30. Soltan-Dallal MM (2001). Diarrhea caused by enteropathogenic bacteria in children. Arch Irn Med., 4(4): 201-203.
31. Soltan Dallal MM, Khorramizadeh MR, Moezardalan K (2006). Occurrence of enteropathogenic bacteria in children under 5 years With diarrhoea in South Tehran, 12(6):792-797.
32. Sunabe T, Honma Y (1998). Relationship between O-serogroup and Presence of pathogenic factor genes in Escherichia coli. Microbiol. Immunol. 42: 845-849.
33. Wood LV, Ferguson LE, Hogan P, Thurman D, Morgan DR, DuPont HL (1983). Incidence of bacterial enteropathogens in foods from Mexico. Appl. Environ. Microbiol., 46(2):328-332.