Monitoring Rhodotorula glutinis CCMI 145 stress physiological response during fed-batch fermentations using multi-parameter flow cytometry

Journal of Microbial and Biochemical Technology

Volumn 3, Issue 1, 2011, Pages 6-12

  da Silva, Teresa Lopes ^a   Feijão, Daniela ^a   Reis, Alberto ^a  

^a LABORATÓRIO NACIONAL DE ENERGIA E GEOLOGIA   (Portugal)

Author keywords

Fed batch fermentation; Light scatter; Multi parameter flow cytometry; Nile red; Propidium iodide; Rhodotorula glutinis

Indexed keywords

LIPID;

ARTICLE; BIOPROCESS; BIOTECHNOLOGICAL PRODUCTION; CARBON SOURCE; CELL VIABILITY; CONTROL SYSTEM; CONTROLLED STUDY; CYTOLYSIS; FED BATCH FERMENTATION; FLOW CYTOMETRY; GROWTH CURVE; LIGHT SCATTERING; MEMBRANE PERMEABILITY; MICROBIAL GROWTH; MULTI PARAMETER FLOW CYTOMETRY; NONHUMAN; PHYSIOLOGICAL STRESS; RHODOTORULA GLUTINIS; YEAST CELL;

RHODOTORULA GLUTINIS;

EID: 79952234996     PISSN: None     EISSN: 19485948     Source Type: Journal    
DOI: 10.4172/1948-5948.1000042     Document Type: Article

References (31)

1. Díaz M, Herrero M, García LA, Quirós C (2009) Application of flow cytometry to industrial microbial bioprocesses. Biochem Eng J 48:385-407.
2. Hewitt CJ, Nebe-Von-Caron G (2004) The application of multi-parameter flow cytometry to monitor individual microbial cell physiological state. Adv Biochem Eng Biotechnol 89:197-223.
3. Novak J, Basarova G, Fiala J, Dostalek P (2008) Flow cytometry in study of the yeast Saccharomyces cerevisiae and its practical application. Chem Listy 102:183-187.
4. Aksu Z, Eren A (2007) Production of carotenoids by the isolated yeast of Rhodotorula glutinis. Biochem Eng J 35:107-113.
5. Frengova G, Beshkova DM (2009) Carotenoids from Rhodotorula and Phaffia: yeasts of biotechnological importance. J Ind Microbiol Biotechnol 36:163-180.
6. Xue F, Miao J, Zhang X, Luo H, Tan T (2008) Studies on lipid production by Rhodotorula glutinis fermentation using monosodium glutamate wastewater as culture medium. Biores Technol 99:5923-5927.
7. Li Q, Du W, Liu D (2008) Perspectives of microbial oils for biodiesel production. Appl Microbiol Biotechnol 80:749-758.
8. Dai C, Tao J, Xie F, Dai Y, Zhao M (2007) Biodiesel generation from oleaginous yeast Rhodotorula glutinis with xylose assimilating capacity. Afr J Biotechnol 6:2130-2134.
9. Easterling EM, French WT, Hernandez R, Lich M (2009) The effect of glycerol as a sole and secondary substrate on the growth and fatty acid composition of Rhodotorula glutinis. Biores Technol 199:356-361.
10. Elsey, D, Jameson D, Raleigh B, Cooney M (2007) Fluorescent measurement of microalgal neutral lipids. J Microbiol Methods 68:639-642.
11. Lopes da Silva T, Feijão D, Reis A (2010) Using multi-parameter flow cytometry to monitor the yeast Rhodotorula glutinis CCMI 145 batch growth and oil production towards biodiesel. Appl Biochem Biotechnol 162:2166-2176.
12. Greenspan P, Mayer E, Fowler S (1985) Nile Red: A selective Fluorescent stain for intracellular lipid droplets. J Cell Biol 100:965-973.
13. de la Jara A, Mendoza H, Martel A, Molina C, Nordströn L, et al. (2003) Flow cytometric determination of lipid content in a marine dinoflagellate Crypthecodinium cohnii. J Appl Phycol 15:433-438.
14. Tornabene TG (1983) Lipid composition of the nitrogen starved green alga Neochloris oleoabundans. Enzyme Microbiol Technol 5:435-440.
15. Sedlak M, Ho N (2004) Production of ethanol from cellulosic biomass hydrolysates using genetically engineered Saccharomyces yeast capable of co-fermenting glucose and xylose. Appl Biochem Biotechnol 113:403-416.
16. Sijtsma L, Anderson A, Ratledge C (2005) Alternative carbon sources for heterotrophic production of docosahexaenoic acid by the marine alga Crypthecodinium cohnii. In Z Cohen Z and Ratledge C (eds.) Single cell oils, AOCS PRESS, Champaign, USA pp. 107-123.
17. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426-428.
18. Lopes da Silva T, Reis A (2008) The use of multi-parameter flow cytometry to study the impact of n-dodecane additions to marine dinoflagellate microalga Crypthecodinium cohnii batch fermentations and DHA production. J Ind Microbiol Biotechnol 35:875-882.
19. Mannazzu I., Angelozzi D, Belviso S, Budroni M, Farris GA, et al. (2008) Behaviour of Saccharomyces cerevisiae wine strains during adaptation to unfavourable conditions of fermentation on synthetic medium: Cell lipid composition, membrane integrity, viability and fermentative activity. Int J Food Microbiol 121:84-91.
20. Marza E, Camougrand N, Manon S (2002) Max expression protects yeast plasma membrane against ethanol-induced permeabilization. FEBS Letters 52:47-52.
21. Lopes da Silva T, Mendes A, Mendes R, Calado V, Alves SS, et al. (2006) Effect of n-dodecane on Crypthecodinium cohnii fermentations and DHA production. J Ind Microbiol Biotechnol 33:408-416.
22. Deere D, Shen J, Vesey G, Bell P, Bissinger P, et al. (1998) Flow Cytometry and cell sorting for yeast viability assessment and cell selection. Yeast 14:147-160.
23. Boswell C, Nienow A, Hewitt CJ (2002) Studies on the effect of mechanical agitation on the performance of brewing fermentations: fermentation rate, yeast physiology, and development of flavour compounds. J Am Soc Brew Chem 60:101-103.
24. Lindegren CC, Bemiller PM, Liu K, Lindegren G (1972) Staining yeast cells for electron microscopy by growth in copper-containing nutrient broth. Antonie Leeuwenhoek 38:17-26.
25. Takeo K, Yarita K, Yoshida S, Guan HQ, Nishimura K, et al. (1993) Rapid, extensive and reversible vacuolation of Schizosaccharomyces pombe induced by amphotericin B. FEMS Microbiol Lett.108:265-269.
26. Yoon SH, Rhee JS (1983) Lipid from yeast fermentation: Effects of cultural conditions on lipid production and its characteristics of Rhodotorula glutinis. JAOCS 60: 1281-1286.
27. Choi SY, Ryu DD, Rhee JS (1982) Production of Microbial Lipid: effects of growth rate and oxygen on lipid synthesis and fatty acid composition of Rhodotorula gracilis. Biotechnol Bioeng 24:1165-1172.
28. Hewitt CJ, Nebe-Von-caron G (2001) An Industrial application of multiparameter flow cytometry: Assessment of cell physiological state and its application to the tidy of microbial fermentations. Cytometry 44:179-187.
29. Ludovico P, Sansonety F, Corte-Real M (2001) Assessment of mitochondrial membrane potential in yeast cell populations by flow cytometry. Microbiology 147:3335-3343.
30. Stauber JL, Franklin NM, Adams MS (2002) Applications of flow cytometry to ecotoxicity testing using microalgae. Tibtech 20:141-143.
31. Yeung PKK, Wong JTY (2003) Inhibition of cell proliferation by mechanical agitation involves transient cell cycle arrest at G1 phase dinoflagellates. Protoplasma 220: 173-178.