Enzyme Stabilization via Cross-Linked Enzyme Aggregates

Methods in Molecular Biology

Volumn 679, Issue , 2011, Pages 133-145

  Gupta, Munishwar N ^a   Raghava, Smita ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

Author keywords

Cross linked enzyme aggregates; Cross linking agents; Enantioselectivity; Glutaraldehyde; Immobilization; Thermal stability

Indexed keywords

CROSS LINKING REAGENT; ENZYME; GLUTARALDEHYDE; GLYCOSIDASE; IMMOBILIZED ENZYME; PENICILLIN AMIDASE; TRIACYLGLYCEROL LIPASE;

ARTICLE; BIOTECHNOLOGY; CHEMISTRY; ENZYME STABILITY; IMMUNOLOGY; METHODOLOGY; SCANNING ELECTRON MICROSCOPY; TEMPERATURE;

BIOTECHNOLOGY; CROSS-LINKING REAGENTS; ENZYME STABILITY; ENZYMES; ENZYMES, IMMOBILIZED; GLUTARAL; GLYCOSIDE HYDROLASES; LIPASE; MICROSCOPY, ELECTRON, SCANNING; PENICILLIN AMIDASE; TEMPERATURE;

MLCS; MLOWN;

EID: 79952200823     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-895-9_11     Document Type: Chapter

References (26)

1. Wold, F. (1967) Bifunctional reagents. Methods Enzymol. 11, 617–640.
2. Broun, G. B. (1976) Chemically aggregated enzymes. Methods Enzymol. 44, 263–280.
3. Gupta, M. N. (1993) Applications of cross-linking techniques to enzyme/protein stabilization and bioconjugate preparation. In Biocatalyst Design for Stability and Specificity (Himmel, M. E., and Georgiou, G., Eds.) pp 307–324, ACS Symposium Series American Chemical Society, Washington, DC.
4. Sheldon, R. A., Schoevaart, R., and van Langen, L. M. (2006) Cross-linked enzyme aggregates. In Immobilization of Enzymes and Cells (Guisan, J. M., Ed.) pp 43, Humana Press, New Jersey.
5. van Langen, L. M., Selassa, R. P., van Rantwijk, F., and Sheldon, R. A. (2005) Cross-linked aggregates of (R)-oxynitrilase: A stable, recyclable biocatalyst for enantioselective hydro-cyanation. Org. Lett. 7, 327–329.
6. Majumder, A. B., Mondal, K., Singh, T. P., and Gupta, M. N. (2008) Designing cross-linked lipase aggregates for optimum performance as biocatalysts. Biocatal. Biotransformation 26, 235–242.
7. Dalal, S., Sharma, A., and Gupta, M. N. (2007) A multipurpose immobilized biocatalyst with pectinase, xylanase and cellulase activities. Chem. Cent. J. 1: 16.
8. Shah, S., Sharma, A., and Gupta, M. N. (2006) Preparation of cross-linked enzyme aggregates by using bovine serum albumin as a proteic feeder. Anal. Biochem. 351, 207–213.
9. Sheldon, R. A. (2006) Immobilization of enzymes as cross-linked enzyme aggregates: A simple method for improving performance. In Biocatalysis in the Pharmaceutical and Biotechnology Industries (Patel, R. N., Ed.) pp 350–362, CRC Press, New York.
10. Illanes, A., Wilson, L., Caballero, E., Fernández-Lafuente, R., and Guisan, J. M. (2006) Cross-linked penicillin acylase aggregates for synthesis of b-lactam antibiotics in organic medium. Appl. Biochem. Biotechnol. 133, 189–202.
11. Ruiz Toral, A., de los Rios, A. P., Hernandez, F. J., Janssen, M. H. A., Schoevaart, R., van Rantwijk, F., and Sheldon, R. A. (2007) Cross-linked Candida antarctica lipase B is active in denaturing ionic liquids. Enzyme Microb. Technol. 40, 1095–1099.
12. 6 ] using high activity preparations of lipases. Bioorg. Med. Chem. Lett. 17, 921–924.
13. 2. Green Chem. 8, 816–821.
14. Mateo, B., Chmura, A., Rustler, S., van Rantwijk, F., Stolz, A., and Sheldon, R. A. (2006) Synthesis of enantiomerically pure (S)-mandelic acid using an oxynitri-lase–nitrilase bienzymatic cascade: a nitrilase surprisingly shows nitrile hydratase activity. Tetrahedron Asymm. 17, 320–323.
15. St. Clair, N. L., and Navia, M. A. (1992) Cross-linked enzyme crystal as robust biocatalysts, J. Am. Chem. Soc. 114, 7314–7316.
16. Mondal, K., Ramesh, N. G., Roy, I., and Gupta, M. N. (2006) Enhancing the synthetic utility of aldolase antibody 38C2. Bioorg. Med. Chem. Lett. 16, 807–810.
17. Wilson, L., Illanes, A., Abian, O., Pessela, B. C. C., Fernandez-Lafuente, R., and Guisán, J. M. (2004) Co-aggregation of penicillin G acylase and polyionic polymers: an easy methodology to prepare enzyme biocatalysts stable in organic media. Biomacromolecules 5, 852–857.
18. Kim, M. I., Kim, J., Lee, J., Jia, H., Na, H. B., Youn, J. K., Kwak, J. H., Dohnalkova, A., Grate, J. W., Wang, P., Hyeon, T., Park, H. G., Chang, H. N. (2007) Cross-linked enzyme aggregates in hierarchically-ordered mesoporous silica: A simple and effective method for enzyme stabilization. Biotechnol. Bioeng. 96, 210–218.
19. Hilal, N., Nigmatullin, R., and Alpatova, A. (2004) Immobilization of cross-linked lipase aggregates within microporous polymeric membranes. J. Memb. Sci. 238, 131–141.
20. Mateo, C., Palomo, J. M., van Langen, L. M., Rantwijik, F. V., and Sheldon, R. A. (2004) A new, mild cross-linking methodology to prepare cross-linked enzyme aggregates. Biotechnol. Bioeng. 86, 273–276.
21. Bell, G., Halling, P. J., Moore, B. D., Partridge, J., and Rees, D. G. (1995) Biocatalyst behavior in low-water systems. Trends Biotechnol. 13, 468–473.
22. Dalal, S., Kapoor, M., and Gupta, M. N. (2007) Preparation and characterization of combi-CLEAs catalyzing multiple non-cascade reactions. J. Mol. Catal. B: Enzym. 44, 128–132.
23. Schoevaart R., Wolbers, M. W., Golubovic, M., Ottens, M., Kieboom, A. P. G., Rantwijik, F. V., van der Wielen, L. A. M., and Sheldon, R. A. (2004) Preparation, optimization, and structures of cross-linked enzyme aggregates (CLEAs). Biotechnol. Bioeng. 20, 754–762.
24. Tyagi, R., Batra, R., and Gupta, M. N. (1999) Amorphous enzyme aggregates: stability towards heat and aqueous-organic cosolvent mixtures. Enzyme Microb. Technol. 24, 348–353.
25. López-Gallego, F., Betancor, L., Hidalgo, A., Alonso, N., Fernandez-Láfuente, R., and Guisán, J. M. (2005) Co-aggregation of enzymes and polyethyleneimine: a simple method to prepare stable and immobilized derivatives of glu-taryl acylase. Biomacromolecules 6, 1639–1842.
26. Vaidya, A., and Fischer, L. (2006) Stabilization of new imprint property of glucose oxidase in pure aqueous medium by cross-linked-imprinting approach. In Immobilization of enzymes and cells (Guisan, J. M., Ed.) pp 175– 183, Humana Press, New Jersey.