Functional Assays for Human Embryonic Stem Cell Pluripotency

Methods in Molecular Biology

Volumn 690, Issue , 2011, Pages 67-80

  O’Connor, Michael D ^a   Kardel, Melanie D ^a   Eaves, Connie J ^a  

^a BRITISH COLUMBIA CANCER AGENCY   (Canada)

Author keywords

Colony forming cell; Embryoid body; Functional assay; Human embryonic stem cell; Induced pluripotent stem cell; Teratoma

Indexed keywords

MUS;

ALKALINE PHOSPHATASE; COLLAGEN; LAMININ; MATRIGEL; PROTEOGLYCAN;

ANIMAL; ARTICLE; CANCER TRANSPLANTATION; CELL DIFFERENTIATION; CELL LINE; CLONOGENIC ASSAY; CYTOLOGY; DRUG COMBINATION; DRUG EFFECT; DRUG SCREENING; EMBRYOID BODY; EMBRYONIC STEM CELL; ENZYMOLOGY; FIBROBLAST; HUMAN; METABOLISM; METHODOLOGY; MOUSE; MOUSE MUTANT; PATHOLOGY; PLURIPOTENT STEM CELL; SUBCUTANEOUS DRUG ADMINISTRATION; TERATOMA;

ALKALINE PHOSPHATASE; ANIMALS; CELL DIFFERENTIATION; CELL LINE; COLLAGEN; COLONY-FORMING UNITS ASSAY; DRUG COMBINATIONS; EMBRYOID BODIES; EMBRYONIC STEM CELLS; FIBROBLASTS; HUMANS; INJECTIONS, SUBCUTANEOUS; LAMININ; MICE; MICE, SCID; NEOPLASM TRANSPLANTATION; PLURIPOTENT STEM CELLS; PROTEOGLYCANS; TERATOMA; TUMOR STEM CELL ASSAY;

EID: 79952199974     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-962-8_4     Document Type: Chapter

References (21)

1. Thomson, J. A., Itskovitz-Eldor, J., Shapiro, S. S., Waknitz, M. A., Swiergiel, J. J., Marshall, V. S., et al. (1998) Embryonic stem cell lines derived from human blastocysts. Science 282, 1145–1147.
2. Reubinoff, B. E., Pera, M. F., Fong, C. Y., Trounson, A., and Bongso, A. (2000) Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat. Biotechnol. 18, 399–404.
3. Ludwig, T. E., Levenstein, M. E., Jones, J. M., Berggren, W. T., Mitchen, E. R., Frane, J. L., et al. (2006) Derivation of human embryonic stem cells in defined conditions. Nat. Biotechnol. 24, 185–187.
4. Ludwig, T. E., Bergendahl, V., Levenstein, M. E., Yu, J., Probasco, M. D., and Thomson, J. A. (2006) Feeder-independent culture of human embryonic stem cells. Nat. Methods 3, 637–646.
5. Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., et al. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861–872.
6. Yu, J., Vodyanik, M. A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J. L., Tian, S., et al. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318, 1917–1920.
7. Park, I. H., Zhao, R., West, J. A., Yabuuchi, A., Huo, H., Ince, T. A., et al. (2008) Reprogramming of human somatic cells to pluripotency with defined factors. Nature 451, 141–146.
8. Lerou, P. H., and Daley, G. Q. (2005) Therapeutic potential of embryonic stem cells. Blood Rev. 19, 321–331.
9. Lensch, M. W., and Daley, G. Q. (2006) Scientific and clinical opportunities for modeling blood disorders with embryonic stem cells. Blood 107, 2605–2612.
10. Daley, G. Q. (2007) Towards the generation of patient-specific pluripotent stem cells for combined gene and cell therapy of hematologic disorders. Hematology Am. Soc. Hematol. Educ. Program 2007, 17–22.
11. Murry, C. E., and Keller, G. (2008) Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development. Cell 132, 661–680.
12. Amit, M., Shariki, C., Margulets, V., and Itskovitz-Eldor, J. (2004) Feeder layer-and serumfree culture of human embryonic stem cells. Biol. Reprod. 70, 837–845.
13. O’Connor, M. D., Kardel, M. D., Iosfina, I., Youssef, D., Lu, M., Li, M. M., et al. (2008) Alkaline phosphatase-positive colony formation is a sensitive, specific and quantitative indicator of undifferentiated human embryonic stem cells. Stem Cells 26, 1109–1116.
14. Ungrin, M., O’Connor, M. D., Eaves, C. J., and Zandstra, P. W. (2007) Phenotypic analysis of human embryonic stem cells. Curr. Protoc. Stem Cell Biol. 2, 3.1–3.25.
15. Palmqvist, L., Glover, C. H., Hsu, L., Lu, M., Bossen, B., Piret, J. M., et al. (2005) Correlation of murine embryonic stem cell gene expression profiles with functional measures of pluripotency. Stem Cells 23, 663–680.
16. Wiles, M. V. (1993) Embryonic stem cell differentiation in vitro. Methods Enzymol. 225, 900–918.
17. Martin, G. R. (1981) Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc. Natl. Acad. Sci. U.S.A. 78, 7634–7638.
18. Takahashi, K., and Yamanaka, S. (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663–676.
19. Itskovitz-Eldor, J., Schuldiner, M., Karsenti, D., Eden, A., Yanuka, O., Amit, M., et al. (2000) Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers. Mol. Med. 6, 88–95.
20. Lawrenz, B., Schiller, H., Willbold, E., Ruediger, M., Muhs, A. and Esser, S. (2004) Highly sensitive biosafety model for stem-cell-derived grafts. Cytotherapy 6, 212–222.
21. Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., et al. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat. Biotechnol. 25, 681–686.