The Immobilization of Enzyme on Eupergit® Supports by Covalent Attachment

Methods in Molecular Biology

Volumn 679, Issue , 2011, Pages 99-111

  Knežević Jugović, Zorica D ^a   Bezbradica, Dejan I ^a   Mijin, Dušan Ž ^a   Antov, Mirjana G ^b  

^a UNIVERSITY OF BELGRADE   (Serbia)

^b UNIVERSITY OF NOVI SAD   (Serbia)

Author keywords

Aminated support; Covalent immobilization; Epoxy groups; Eupergit C; Eupergit C 250L; Glycoenzymes; Periodate oxidation

Indexed keywords

EUPERGIT C; GLUTARALDEHYDE; IMMOBILIZED ENZYME; MICROSPHERE; PERIODATE; PERIODIC ACID; POLYMER; TRIACYLGLYCEROL LIPASE;

ARTICLE; BIOTECHNOLOGY; CHEMICAL STRUCTURE; CHEMISTRY; METHODOLOGY; OXIDATION REDUCTION REACTION; POROSITY;

BIOTECHNOLOGY; ENZYMES, IMMOBILIZED; GLUTARAL; LIPASE; MICROSPHERES; MOLECULAR STRUCTURE; OXIDATION-REDUCTION; PERIODIC ACID; POLYMERS; POROSITY;

MLCS; MLOWN;

EID: 79952198628     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-895-9_9     Document Type: Chapter

References (15)

1. Sheldon, R. A. (2007) Enzyme immobilization: The quest for optimum performance. Adv. Synth. Catal. 349, 1289–1307.
2. Balcao V. M., Paiva A. L., and Malcata X. F. (1996) Bioreactors with immobilized lipases: State of the art. Enzyme Microb. Technol. 18, 392–416.
3. Malcata, X., Reyes, H., Garcia, H., Hill, C., and Amundson, C. (1992). Kinetics and mechanisms of reactions catalyzed by immobilized lipases. Enzyme Microb. Technol. 14, 426–446.
4. ® C, a carrier for immobilization of enzymes of industrial potential. J. Mol. Catal. B Enzym. 10, 157–176.
5. Hernaiz, M. J. and Crout, D. H. G. (2000) Immobilization/stabilization on Eupergit C of the b-galactosidase from B. circulans and an a-galactosidase from Aspergillus oryzae. Enzyme Microb. Technol. 27, 26–32.
6. Rocchietti, S., San Vicente Urrutia, A., Pregnolato, M., Tagliani, A., Guissan, J. M., Fernandez-Lafuente, R., and Terreni, M. (2002) Influence of the enzyme derivative preparation and substrate structure on the enantioselectivity of penicillin G acylase. Enzyme Microb. Technol. 31, 88–93.
7. Wirz, B., Barner, R., and Huebscher, J. (1993) Facile chemoenzymic preparation of enantiomerically pure 2-methylglycerol derivatives as versatile trifunctional C4-synthons. J. Am. Chem. Soc. 58, 3980–3984.
8. Hilal N., Kochkodan V., Nigmatullin R., Goncharuk V., and Al-Khatib L. (2006) Lipase-immobilized biocatalytic membranes for enzymatic esterification: Comparison of various approaches to membrane preparation. J. Membr. Sci. 268, 198–207.
9. Mateo C., Abian O., Bernedo M., Cuenca E., Fuentes M., Fernandez-Lorente G., Palomo J. M., Grazu V., Pessela B., Giacomini C., Irazoqui G., Villarino A., Ovsejevi K., Batista-Viera F., Fernandez-Lorente R., and Guisan J. M. (2005) Some special features of glyoxyl supports to immobilize proteins. Enzyme Microb. Technol. 37, 456–462.
10. ® C supports by covalent attachment. Biochem. Eng. J. 30, 269–278.
11. Licia M. P., Cintia M. R., Mario D. B., and Guillermo R. C. (2006) Catalytic Properties of Lipase Extracts from Aspergillus niger. Food Technol. Biotechnol. 44, 247–252.
12. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951) Protein measurement with Folin-phanol reagent. J. Biol. Chem. 193, 265–275.
13. Bradford, M. M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.
14. ® supports. J. Chem. Technol. Biotechnol. 84, 1642–1648.
15. Fernandez-Lafuente, R., Rosell, C. M., Rodrıguez, V., Santana, C., Soler, G., Bastida, A., and Guisan, J. M. (1993) Preparation of activated supports containing low pK amino groups. A new tool for protein immobilization via the carboxyl coupling method. Enzyme Microb. Technol. 15, 546–550.