Detection of MicroRNAs in Cultured Cells and Paraffin-Embedded Tissue Specimens by In Situ Hybridization

Methods in Molecular Biology

Volumn 676, Issue , 2011, Pages 73-83

  Gupta, Ashim ^a   Mo, Yin Yuan ^a  

^a SOUTHERN ILLINOIS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Expression; In situ hybridization; Locked nucleic acid; MicroRNA

Indexed keywords

MICRORNA; PARAFFIN;

ARTICLE; GENETICS; HUMAN; IN SITU HYBRIDIZATION; METHODOLOGY; TUMOR CELL LINE;

CELL LINE, TUMOR; HUMANS; IN SITU HYBRIDIZATION; MICRORNAS; PARAFFIN EMBEDDING;

MLCS; MLOWN;

EID: 79952198581     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-863-8_6     Document Type: Chapter

References (16)

1. Pillai, R. S. (2005) MicroRNA function: multiple mechanisms for a tiny RNA? RNA 11, 175361.
2. Zamore, P. D. and Haley, B. (2005) Ribo-gnome: the big world of small RNAs. Science 309, 1519–24.
3. Bartel, D. P. (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116, 281–97.
4. Ambros, V. (2004) The functions of animal microRNAs. Nature 431, 350–5.
5. Lee, R. C., Feinbaum, R. L., and Ambros, V. (1993) The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense com-plementarity to lin-14. Cell 75, 843–54.
6. Wightman, B., Ha, I., and Ruvkun, G. (1993) Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans. Cell 75, 855–62.
7. Reinhart, B. J., Slack, F. J., Basson, M., Pasquinelli, A. E., Bettinger, J. C., Rougvie, A. E., Horvitz, H. R., and Ruvkun, G. (2000) The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. Nature 403, 901–6.
8. Griffiths-Jones, S. (2004) The microRNA Registry. Nucleic Acids Res 32, D109–11.
9. Yamamichi, N., Shimomura, R., Inada, K., Sakurai, K., Haraguchi, T., Ozaki, Y., Fujita, S., Mizutani, T., Furukawa, C., Fujishiro, M., Ichinose, M., Shiogama, K., Tsutsumi, Y., Omata, M., and Iba, H. (2009) Locked nucleic acid in situ hybridization analysis of miR-21 expression during colorectal cancer development. Clin Cancer Res 15, 4009–16.
10. Kloosterman, W. P., Wienholds, E., de Bruijn, E., Kauppinen, S., and Plasterk, R. H. (2006) In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes. Nat Methods 3, 27–9.
11. Pena, J. T., Sohn-Lee, C., Rouhanifard, S. H., Ludwig, J., Hafner, M., Mihailovic, A., Lim, C., Holoch, D., Berninger, P., Zavolan, M., and Tuschl, T. (2009) miRNA in situ hybridization in formaldehyde and EDC-fixed tissues. Nat Methods 6, 139–41.
12. Kauppinen, S., Vester, B., and Wengel, J. (2006) Locked nucleic acid: high-affinity targeting of complementary RNA for RNomics. Handb Exp Pharmacol 173, 405–22.
13. Koshkin, A. A. and Wengel, J. (1998) Synthesis of novel 2¢,3¢-linked bicyclic thymine ribonu-cleosides. J Org Chem 63, 2778–81.
14. Wienholds, E., Kloosterman, W. P., Miska, E., Alvarez-Saavedra, E., Berezikov, E., de Bruijn, E., Horvitz, H. R., Kauppinen, S., and Plasterk, R. H. (2005) MicroRNA expression in zebrafish embryonic development. Science 309, 310–1.
15. Nelson, P. T., Baldwin, D. A., Kloosterman, W. P., Kauppinen, S., Plasterk, R. H., and Mourelatos, Z. (2006) RAKE and LNA-ISH reveal microRNA expression and localization in archival human brain. RNA 12, 187–91.
16. van Gijlswijk, R. P., Wiegant, J., Vervenne, R., Lasan, R., Tanke, H. J., and Raap, A. K. (1996) Horseradish peroxidase-labeled oligonucleotides and fluorescent tyramides for rapid detection of chromosome-specific repeat sequences. Cytogenet Cell Genet 75, 258–62.