Embryoid Body Formation: Recent Advances in Automated Bioreactor Technology

Methods in Molecular Biology

Volumn 690, Issue , 2011, Pages 135-149

  Trettner, Susanne ^a   Seeliger, Alexander ^a   Nieden, Nicole I zur ^a,b  

^a FRAUNHOFER INSTITUTE FOR CELL THERAPY AND IMMUNOLOGY   (Germany)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Bioreactor; Embryoid body; Osteogenesis; Suspension

Indexed keywords

BIOLOGICAL MARKER; BRACHYURY PROTEIN; FETOPROTEIN; MINERAL; T BOX TRANSCRIPTION FACTOR;

ANIMAL; ARTICLE; AUTOMATION; BIOREACTOR; CALIBRATION; CELL DIFFERENTIATION; CELL LINEAGE; CULTURE TECHNIQUE; CYTOLOGY; EMBRYOID BODY; INSTRUMENT STERILIZATION; METABOLISM; METHODOLOGY; MOUSE; OSTEOBLAST;

ANIMALS; AUTOMATION; BIOLOGICAL MARKERS; BIOREACTORS; CALIBRATION; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINEAGE; EMBRYOID BODIES; FETAL PROTEINS; MICE; MINERALS; OSTEOBLASTS; STERILIZATION; T-BOX DOMAIN PROTEINS;

EID: 79952198552     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-962-8_9     Document Type: Chapter

References (18)

1. Ying, Q.L., Wray, J., Nichols, J., Batlle-Morera, L., Doble, B., Woodgett, J., et al. (2008) The ground state of embryonic stem cell self-renewal. Nature 453, 519–523.
2. Martin, G.R. and Evans, M.J. (1975) Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc. Natl. Acad. Sci. U.S.A. 72, 1441–1445.
3. Martin, G.R. (1981) Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc. Natl. Acad. Sci. U.S.A. 78, 7634–7638.
4. Lin, R.Y., Kubo, A., Keller, G.M., and Davies, T.F. (2003) Committing embryonic stem cells to differentiate into thyrocyte-like cells in vitro. Endocrinology 144, 2644–2649.
5. Bratt-Leal, A.M., Carpenedo, R.L., and McDevitt, T.C. (2009) Engineering the embryoid body microenvironment to direct embryonic stem cell differentiation. Biotechnol. Prog. 25, 43–51.
6. Wobus, A.M., Wallukat, G., and Hescheler, J. (1991) Pluripotent mouse embryonic stem cells are able to differentiate into cardiomyocytes expressing chronotropic responses to adrenergic and cholinergic agents and Ca2+ channel block-ers. Differentiation 48, 173–182.
7. zur Nieden, N.I., Kempka, G., Rancourt, D.E., and Ahr, H.J. (2005) Induction of chondro-, osteo-and adipogenesis in embryonic stem cells by bone morphogenetic protein-2: effect of cofactors on differentiating lineages. BMC Dev. Biol. 5, 1.
8. Strübing, C., Ahnert-Hilger, G., Shan, J., Wiedenmann, B., Hescheler, J., and Wobus, A.M. (1995) Differentiation of pluripotent embryonic stem cells into the neuronal lineage in vitro gives rise to mature inhibitory and excitatory neurons. Mech. Dev. 53, 275–287.
9. Rose, O., Rohwedel, J., Reinhardt, S., Bachmann, M., Cramer, M., Rotter, M., et al. (1994) Expression of M-cadherin protein in myogenic cells during prenatal mouse development and differentiation of embryonic stem cells in culture. Dev. Dyn. 201, 245–259.
10. Scholz, G., Pohl, I., Genschow, E., Klemm, M., and Spielmann, H. (1999) Embryotoxicity screening using embryonic stem cells in vitro: correlation to in vivo teratogenicity. Cells Tissues Organs 165, 203–211.
11. Fok, E.Y.L. and Zandstra, P.W. (2005) Shear-controlled single-step mouse embryonic stem cell expansion and embryoid body-based differentiation. Stem Cells 23, 1333–1342.
12. Cormier, J.T., zur Nieden, N.I., Rancourt, D.E., and Kallos, M.S. (2006) Expansion of undifferentiated murine embryonic stem cells as aggregates in suspension culture bioreactors. Tissue Eng. 12, 3233–3245.
13. zur Nieden, N.I., Cormier, J.T., Rancourt, D.E., and Kallos, M.S. (2007) Embryonic stem cells remain highly pluripotent following long term expansion as aggregates in suspension bioreactors. J. Biotechnol. 129, 421–432.
14. Karp, J.M., Yeh, J., Eng, G., Fukuda, J., Blumling, J., Suh, K.Y., et al. (2007) Controlling size, shape, and homogeneity of embryoid bodies using poly(ethylene glycol) microwells. Lab Chip 7, 786–794.
15. Youn, B.S., Sen, A., Behie, L.A., Girgis-Gabardo, A., and Hassell, J.A. (2006) Scaleup of breast cancer stem cell aggregate cultures to suspension bioreactors. Biotechnol. Prog. 22, 801–810.
16. Zhao, F., Pathi, P., Grayson, W., Xing, Q., Locke, B.R., and Ma, T. (2005) Effects of oxygen transport on 3-D human mesenchymal stem cell metabolic activity in perfusion and static cultures: experiments and mathematical model. Biotechnol. Prog. 21, 1269–1280.
17. zur Nieden, N.I., Kempka, G., and Ahr, H.J. (2003) In vitro differentiation of embryonic stem cells into mineralized osteoblasts. Differentiation 71, 18–27.
18. Ward, C.M., Barrow, K., Woods, A.M., and Stern, P.L. (2003) The 5T4 oncofoetal antigen is an early differentiation marker of mouse ES cells and its absence is a useful means to assess pluripotency. J. Cell. Sci. 116, 4533–4542.