메뉴 건너뛰기




Volumn 20, Issue 1, 2011, Pages 52-62

Nucleic acid quality preservation by an alcohol-based fixative: Comparison with frozen tumors in a routine pathology setting

Author keywords

alcohol based fixative; nucleic acid preservation; paraffin embedded tissue; tissue fixation

Indexed keywords

ALCOHOL; DNA; FIXATIVE; FORMALDEHYDE; NUCLEIC ACID; RNA;

EID: 79952120614     PISSN: 10529551     EISSN: None     Source Type: Journal    
DOI: 10.1097/PDM.0b013e3181e71ba5     Document Type: Article
Times cited : (14)

References (27)
  • 1
    • 0036898005 scopus 로고    scopus 로고
    • Effect of fixatives and tissue processing on the content and integrity of nucleic acids
    • Srinivasan M, Sedmak D, Jewel S. Effect of fixatives and tissue processing on the content and integrity of nucleic acids. Am J Pathol 2002;161:1961-1971. (Pubitemid 35434840)
    • (2002) American Journal of Pathology , vol.161 , Issue.6 , pp. 1961-1971
    • Srinivasan, M.1    Sedmak, D.2    Jewell, S.3
  • 2
    • 0033571272 scopus 로고    scopus 로고
    • Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology applications for such samples
    • DOI 10.1093/nar/27.22.4436
    • Masuda N, Ohnishi T, Kawamoto S, et al. Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology applications for such samples. Nucleic Acid Res. 1999;27:4436-4443. (Pubitemid 29533185)
    • (1999) Nucleic Acids Research , vol.27 , Issue.22 , pp. 4436-4443
    • Masuda, N.1    Ohnishi, T.2    Kawamoto, S.3    Monden, M.4    Okubo, K.5
  • 4
    • 17944390807 scopus 로고    scopus 로고
    • Optimisation of DNA and RNA extraction from archival formalin-fixed tissue
    • Coombs NJ, Gough AC, Primrose JN. Optimisation of DNA and RNA extraction from archival formalin-fixed tissue. Nucleic Acid Res. 1999;27:e12.
    • (1999) Nucleic Acid Res , vol.27
    • Coombs, N.J.1    Gough, A.C.2    Primrose, J.N.3
  • 5
    • 17944390807 scopus 로고    scopus 로고
    • Optimisation of DNA and RNA extraction from archival formalin-fixed tissue
    • Coombs NJ, Gough AC, Primrose JN. Optimisation of DNA and RNA extraction from archival formalin-fixed tissue. Nucleic Acid Res. 1998;27:e12.
    • (1998) Nucleic Acid Res , vol.27
    • Coombs, N.J.1    Gough, A.C.2    Primrose, J.N.3
  • 6
    • 33751381986 scopus 로고    scopus 로고
    • Optimization of recovery of RNA from formalin-fixed, paraffin-embedded tissue
    • DOI 10.1097/01.pdm.0000213468.91139.2d, PII 0001960620061200000006
    • Chung JY, Braunschweig T, Hewit SM. Optimization of recovery of RNA from formalin-fixed, paraffin-embedded tissue. Diagn Mol Pathol. 2006;15:229-236. (Pubitemid 44813662)
    • (2006) Diagnostic Molecular Pathology , vol.15 , Issue.4 , pp. 229-236
    • Chung, J.-Y.1    Braunschweig, T.2    Hewitt, S.M.3
  • 7
    • 0036736248 scopus 로고    scopus 로고
    • Effect of duration of fixation on quantitative reverse transcription polymerase chain reaction analyses
    • Macabeo-Ong M, Ginzinger DG, Dekker N, et al. Effect of duration of fixation on quantitative reverse transcription polymerase chain reaction analyses. Mod Pathol. 2002;15:979-987.
    • (2002) Mod Pathol , vol.15 , pp. 979-987
    • Macabeo-Ong, M.1    Ginzinger, D.G.2    Dekker, N.3
  • 8
    • 0034812285 scopus 로고    scopus 로고
    • Unlocking the archive - Gene expression in paraffin-embedded tissue
    • DOI 10.1002/1096-9896(200109)195:1<66::AID-PATH921>3.0.CO;2-F
    • Lewis F, Maughan NJ, Smith V, et al. Unlocking the archivegene expression in paraffin-embedded tissue. J Pathol. 2001;1956: 66-71. (Pubitemid 32899046)
    • (2001) Journal of Pathology , vol.195 , Issue.1 , pp. 66-71
    • Lewis, F.1    Maughan, N.J.2    Smith, V.3    Hillan, K.4    Quirke E, P.5
  • 11
    • 49749122669 scopus 로고    scopus 로고
    • Rapid-response, molecularfriendly surgical pathology: A radical departure from the century old routine practice
    • Morales AR, Nadji M, Livingstone AS. Rapid-response, molecularfriendly surgical pathology: a radical departure from the century old routine practice. J Am Coll Surg. 2008;207:320-325.
    • (2008) J Am Coll Surg , vol.207 , pp. 320-325
    • Morales, A.R.1    Nadji, M.2    Livingstone, A.S.3
  • 12
    • 1642398708 scopus 로고    scopus 로고
    • Experience with an Automated Microwave-Assisted Rapid Tissue Processing Method: Validation of Histologic Quality and Impact on the Timeliness of Diagnostic Surgical Pathology
    • DOI 10.1309/ACK8-AHV0-1T47-QR53
    • Morales AR, Nassiri M, Kanhoush R, et al. Experience with an automated microwave-assisted rapid tissue processing method: validation of histological quality and impact on ther timeliness of diagnostic surgical pathology. Am J Clin Pathol. 2004;121: 528-536. (Pubitemid 38392960)
    • (2004) American Journal of Clinical Pathology , vol.121 , Issue.4 , pp. 528-536
    • Morales, A.R.1    Nassiri, M.2    Kanhoush, R.3    Vincek, V.4    Nadji, M.5
  • 13
    • 40849118177 scopus 로고    scopus 로고
    • Preservation of biomolecules in breast cancer tissue by a formalin-free histology system
    • Nassiri M, Ramos S, Zohourian H, et al. Preservation of biomolecules in breast cancer tissue by a formalin-free histology system. BMC Clin Pathol. 2008;8:1-10.
    • (2008) BMC Clin Pathol , vol.8 , pp. 1-10
    • Nassiri, M.1    Ramos, S.2    Zohourian, H.3
  • 14
    • 33645241407 scopus 로고    scopus 로고
    • The RIN: An RNA integrity number for assigning integrity values to RNA measurements
    • Schroeder A, Mueller O, Stocker S, et al. The RIN: an RNA integrity number for assigning integrity values to RNA measurements. BMC Mol Biol. 2006;7:3.
    • (2006) BMC Mol Biol , vol.7 , pp. 3
    • Schroeder, A.1    Mueller, O.2    Stocker, S.3
  • 16
    • 0035710746 scopus 로고    scopus 로고
    • -ΔΔCT method
    • DOI 10.1006/meth.2001.1262
    • Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and 2-ddCt method. Methods. 2001;25:402-408. (Pubitemid 34164012)
    • (2001) Methods , vol.25 , Issue.4 , pp. 402-408
    • Livak, K.J.1    Schmittgen, T.D.2
  • 17
    • 0035710433 scopus 로고    scopus 로고
    • Real-time PCR analysis of DNA and RNA extracted from formalin-fixed and paraffin-embedded biopsies
    • DOI 10.1006/meth.2001.1263
    • Lehmann U, Kreipe H. Real-time PCR analysis of DNA and RNA extracted from formalin-fixed and paraffin-embedded biopsies. Methods. 2001;25:409-418. (Pubitemid 34164013)
    • (2001) Methods , vol.25 , Issue.4 , pp. 409-418
    • Lehmann, U.1    Kreipe, H.2
  • 18
    • 0032755991 scopus 로고    scopus 로고
    • A high frequency of sequence alterations is due to formalin fixation of archival specimens
    • Williams C, Ponten F, Moberg C, et al. A high frequency of sequence alterations is due to formalin fixation of archival specimens. Am J Pathol. 1999;155:1467-1471.
    • (1999) Am J Pathol , vol.155 , pp. 1467-1471
    • Williams, C.1    Ponten, F.2    Moberg, C.3
  • 19
    • 25844474594 scopus 로고    scopus 로고
    • Methodology for preservation of high molecular-weight RNA in paraffin-embedded tissue
    • Vincek V, Nassiri M, Block N, et al. Methodology for preservation of high molecular-weight RNA in paraffin-embedded tissue. Diagn Mol Pathol. 2005;14:127-133.
    • (2005) Diagn Mol Pathol , vol.14 , pp. 127-133
    • Vincek, V.1    Nassiri, M.2    Block, N.3
  • 20
    • 0142106515 scopus 로고    scopus 로고
    • A Tissue Fixative that Protects Macromolecules (DNA, RNA, and Protein) and Histomorphology in Clinical Samples
    • DOI 10.1097/01.LAB.0000090154.55436.D1
    • Vincek V, Nassiri M, Nadji M, et al. A tissue fixative that protects macromolecules (DNA, RNA, and protein) and histomorphology in clinical samples. Lab Invest. 2005;85:1427-1435. (Pubitemid 37267826)
    • (2003) Laboratory Investigation , vol.83 , Issue.10 , pp. 1427-1435
    • Vincek, V.1    Nassiri, M.2    Nadji, M.3    Morales, A.R.4
  • 21
    • 34547645570 scopus 로고    scopus 로고
    • Novel zinc-based fixative for high quality DNA RNA and protein analysis
    • Lykidis D, van Noorden S, Armstrong A, et al. Novel zinc-based fixative for high quality DNA, RNA and protein analysis. Nucleic Acid Res. 2007;35:e85.
    • (2007) Nucleic Acid Res , vol.35
    • Lykidis, D.1    Van Noorden, S.2    Armstrong, A.3
  • 22
    • 55149100048 scopus 로고    scopus 로고
    • Histology without formalin
    • Buesa RJ. Histology without formalin. Ann Diagn Pathol. 2008; 12:387-396.
    • (2008) Ann Diagn Pathol , vol.12 , pp. 387-396
    • Buesa, R.J.1
  • 23
    • 33746404835 scopus 로고    scopus 로고
    • A novel fixative improves opportunities of nucleic acids and proteomic analysis in human archive's tissues
    • DOI 10.1097/00019606-200606000-00009, PII 0001960620060600000009
    • Stanta G, Mucelli SP, Petrena F, et al. A novel fixative improves opportunities of nucleic acids and proteomic analysis in human archive's tissues. Diagn Mol Pathol. 2006;15:115-123. (Pubitemid 44350489)
    • (2006) Diagnostic Molecular Pathology , vol.15 , Issue.2 , pp. 115-123
    • Stanta, G.1    Mucelli, S.P.2    Petrera, F.3    Bonin, S.4    Bussolati, G.5
  • 26
    • 66349126659 scopus 로고    scopus 로고
    • Morphological quality and nucleic acid preservation in cytopathology
    • Gazziero A, Guzzardo V, Aldighieri E, et al. Morphological quality and nucleic acid preservation in cytopathology. J Clin Pathol. 2009;62:429-434.
    • (2009) J Clin Pathol , vol.62 , pp. 429-434
    • Gazziero, A.1    Guzzardo, V.2    Aldighieri, E.3
  • 27
    • 33645032798 scopus 로고    scopus 로고
    • Methacarn fixation-effects of tissue processing and storage conditions on detection of mRNAs and proteins in paraffin-embedded tissues
    • Lee KY, Shibutani M, Inoue K, et al. Methacarn fixation-effects of tissue processing and storage conditions on detection of mRNAs and proteins in paraffin-embedded tissues. Anal Biochem. 2006;35:36-43.
    • (2006) Anal Biochem , vol.35 , pp. 36-43
    • Lee, K.Y.1    Shibutani, M.2    Inoue, K.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.