Quantifying the Amount and Activity of Rubisco in Leaves

Methods in Molecular Biology

Volumn 684, Issue , 2011, Pages 349-362

  Kubien, David S ^a   Brown, Christopher M ^a   Kane, Heather J ^a  

^a UNIVERSITY OF NEW BRUNSWICK   (Canada)

Author keywords

14C CABP; 14CO2; Photosynthesis; Quantitative immunoblotting; Spectrophotometry

Indexed keywords

2 CARBOXYARABINITOL 1,5 BIPHOSPHATE; 2-CARBOXYARABINITOL 1,5-BIPHOSPHATE; BICARBONATE; CARBON DIOXIDE; PENTOSE PHOSPHATE; RIBULOSEBISPHOSPHATE CARBOXYLASE; SUGAR ALCOHOL;

ARTICLE; CHROMATOGRAPHY; ENZYME ACTIVATION; ENZYME ASSAY; ENZYMOLOGY; METABOLISM; METHODOLOGY; PLANT; PLANT LEAF; POLYACRYLAMIDE GEL ELECTROPHORESIS; SPECTROPHOTOMETRY; WESTERN BLOTTING;

BLOTTING, WESTERN; CARBON DIOXIDE; CHROMATOGRAPHY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; ENZYME ASSAYS; PENTOSEPHOSPHATES; PLANT LEAVES; PLANTS; RIBULOSE-BISPHOSPHATE CARBOXYLASE; SODIUM BICARBONATE; SPECTROPHOTOMETRY; SUGAR ALCOHOLS;

MLCS; MLOWN;

EID: 79952118561     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-925-3_27     Document Type: Chapter

References (21)

1. Ellis, R.J. (1979) The most abundant protein in the world. Trends Biochem. Sci. 4, 241–244.
2. Björkman, O. (1968) Carboxydismutase activity in shade and sun adapted species of higher plants. Physiol. Plant. 21, 1–10.
3. 2, and ribulose-1, 5 disphosphate carboxylase. Plant Physiol. 54, 678–685.
4. 4 photosynthesis at low temperature: a study using transgenic plants with reduced amounts of Rubisco. Plant Physiol. 132, 1577–1585.
5. von Caemmerer, S. (2000) Biochemical Models of Leaf Photosynthesis. CSIRO Publishing.
6. Collatz, G.J., Badger, M., Smith, C., and Berry, J.A. (1979) A radioimmune assay for RuP2 carboxylase protein. Carneige Inst. Wash. Year B. 78, 171–175.
7. 3 photosynthesis studied by rapid-response gas exchange using transgenic tobacco impaired in photosynthesis. Aust. J. Plant Physiol. 25, 859–870.
8. Campbell, D.A., Cockshutt, A.M., and Porankiewicz-Asplund, J. (2003) Analysing photosynthetic complexes in uncharacterized species or mixed microalgal communities using global antibodies. Physiol. Plant. 119, 322–327.
9. Morash, A.J., Campbell, D.A., and Ireland, R.J. (2007) Macromolecular dynamics of the photosynthetic system over a seasonal developmental progression in Spartina alterniflora. Can. J. Bot. 85, 476–483.
10. Rabin, N.G. and Trown, P.W. (1964) Mechanism of action of carboxydismutase. Nature 202, 1290–1293.
11. Paulsen, J.M. and Lane, M.D. (1966) Spinach ribulose diphosphate carboxylase I. Purification and properties of the enzyme. Biochem. 5, 2350–2357.
12. 2 and temperature on a high-affinity form of ribulose disphosphate carboxylase-oxy-genase from spinach. Biochem. Biophys. Res. Comm. 60, 204–210.
13. Seemann, J.R., Badger, M.R., and Berry, J.A. (1984) Variations in the specific activity of ribulose-1, 5-bisphosphate carboxylase between species utilising different photosynthetic pathways. Plant Physiol. 74, 791–794.
14. Lilley, R. McC. and Walker, D.A. (1974) An improved spectrophotometric assay for ribulosebisphosphate carboxylase. Biochim. Biophys. Acta. 358, 226–229.
15. Sharkey, T.D., Savitch, L.V., and Butz, N.D. (1991) Photometric method for routine determination of kcat and carbamylation of Rubisco. Photosyn. Res. 28, 41–48.
16. Keys, A.J. and Parry, M.A.J. (1990) Ribulose bisphosphate carboxylase/oxygenase and carbonic anhydrase. In: Methods in Plant Biochemistry. Vol. 3 (Dey, P.M. and Harborne, J.B., eds.), pp. 1–14.
17. Butz, N.D. and Sharkey, T.D. (1989) Activity ratios of ribulose-1, 5-bisphosphate carboxylase accurately reflect carbamylation ratios. Plant Physiol. 89, 735–739.
18. Reid, C.D., Tissue D.T., Fiscus, E.L., and Strain, B.R. (1997) Comparison of spectrophotometric and radioisotopic methods for the assay of Rubisco in ozone-treated plants. Phys. Plant. 101, 398–404.
19. Kane, H.K., Wilkin, J.M., Portis, A.R., and Andrews, T.J. (1998) Potent inhibition of ribulose-bisphosphate carboxylase by an oxidized impurity in ribulose-1, 5-bisphosphate. Plant Physiol. 117, 1059–1069.
20. Brown, C.M., MacKinnon, J.D., Cockshutt, A.M., Villareal, T., and Campbell, D.A. (2008) Flux capacities and acclimation costs in Trichodesmium from the Gulf of Mexico. Mar. Biol. 154, 413–422.
21. Brown, C.M., Campbell, D.A., and Lawrence, J.E. (2007) Resource dynamics during infection of Micromonas pusilla by virus MpV-Sp1. Environ. Microbiol. 9, 2720–2727.