Carbohydrate binding sites in Candida albicans exo-β-1,3-glucanase and the role of the Phe-Phe 'clamp' at the active site entrance

FEBS Journal

Volumn 277, Issue 21, 2010, Pages 4549-4561

  Patrick, Wayne M ^a,b   Nakatani, Yoshio ^a   Cutfield, Susan M ^a   Sharpe, Miriam L ^a   Ramsay, Rochelle J ^c   Cutfield, John F ^a  

^a UNIVERSITY OF OTAGO   (New Zealand)

^b MASSEY UNIVERSITY   (New Zealand)

^c MASSEY UNIVERSITY   (New Zealand)

Author keywords

Aromatic entranceway clamp; Exoglucanase; Glycoside hydrolase; Ordered water molecules; Protein carbohydrate interaction; Site directed mutagenesis

Indexed keywords

CARBOHYDRATE; GLUCAN 1,3 BETA GLUCOSIDASE; PHENYLALANINE; TRYPTOPHAN; TYROSINE; BETA 1,3 GLUCAN; BETA GLUCAN; BETA-1,3-D-GLUCAN; DIPEPTIDE; FUNGAL PROTEIN; GLUCOSE; OLIGOSACCHARIDE; PHENYLALANYLPHENYLALANINE;

ARTICLE; BINDING SITE; CANDIDA ALBICANS; CARBOHYDRATE ANALYSIS; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME SUBSTRATE COMPLEX; GLYCOSYLATION; HYDROGEN BOND; NONHUMAN; PRIORITY JOURNAL; PROTEIN CARBOHYDRATE INTERACTION; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; STEADY STATE; WILD TYPE; AMINO ACID SUBSTITUTION; CELL WALL; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; X RAY CRYSTALLOGRAPHY;

CANDIDA ALBICANS;

AMINO ACID SUBSTITUTION; BETA-GLUCANS; BINDING SITES; CANDIDA ALBICANS; CARBOHYDRATE SEQUENCE; CELL WALL; CRYSTALLOGRAPHY, X-RAY; DIPEPTIDES; FUNGAL PROTEINS; GLUCAN 1,3-BETA-GLUCOSIDASE; GLUCOSE; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; OLIGOSACCHARIDES; PHENYLALANINE; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; SUBSTRATE SPECIFICITY;

EID: 79952112523     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2010.07869.x     Document Type: Article

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