Characterization of virulence factors and clonal diversity of Enterococcus faecalis isolates from treated dental root canals

Research in Microbiology

Volumn 162, Issue 2, 2011, Pages 151-158

  Zoletti, Gustavo O ^a   Pereira, Eliezer M ^a,c   Schuenck, Ricardo P ^a   Teixeira, Lúcia M ^a   Siqueira, José F ^b   Dos Santos, Kátia Regina N ^a  

^a FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^b ESTÁCIO DE SÁ UNIVERSITY   (Brazil)

^c Federal Institute of Education   (Brazil)

Author keywords

Endodontic infection; Enterococcus faecalis; Genotype patterns; Virulence factors

Indexed keywords

GELATINASE;

ACE GENE; ADULT; AGED; AGG GENE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM ISOLATION; BIOFILM; CLINICAL ARTICLE; CLONAL VARIATION; CPD GENE; EFAA GENE; ENTEROCOCCUS FAECALIS; ESP GENE; FEMALE; HUMAN; MALE; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; TOOTH PERIAPICAL DISEASE; TOOTH ROOT CANAL; WESTERN BLOTTING;

ADULT; AGED; BACTERIAL PROTEINS; DENTAL PULP CAVITY; ELECTROPHORESIS, GEL, PULSED-FIELD; ENTEROCOCCUS FAECALIS; FEMALE; GELATINASES; GENETIC VARIATION; GRAM-POSITIVE BACTERIAL INFECTIONS; HUMANS; MALE; MEMBRANE PROTEINS; MIDDLE AGED; PERIAPICAL PERIODONTITIS; POLYMERASE CHAIN REACTION; TOOTH; VIRULENCE; VIRULENCE FACTORS; YOUNG ADULT;

ENTEROCOCCUS FAECALIS;

EID: 79952105339     PISSN: 09232508     EISSN: 17697123     Source Type: Journal    
DOI: 10.1016/j.resmic.2010.09.018     Document Type: Article

References (38)

1. Baldassari L., Creti R., Arciola C.R., Montanaro L., Venditti M., Di Rosa R. Analysis of virulence factors in cases of enterococcal endocarditis. Clin. Microbiol. Infect. 2004, 10:1006-1008.
2. Carniol K., Gilmore M. Signal transduction, quorum-sensing, and extracellular protease activity in Enterococcus faecalis biofilm formation. J. Bacteriol. 2004, 186:8161-8163.
3. Coque T.M., Patterson J.E., Stecklberg J.M., Murray B.E. Incidence of hemolysin, gelatinase, and aggregation substance among enterococci isolated from patients with endocarditis and other infections and from feces of hospitalized and community-based persons. J. Infect. Dis. 1995, 171:1223-1229.
4. Duprè I., Zanetti A.M., Schito G., Fadda G., Sechi L.A. Incidence of virulence determinants in clinical Enterococcus faecium and Enterococcus faecalis isolates collected in Sardinia (Italy). J. Med. Microbiol. 2003, 52:491-498.
5. Eaton T.J., Gasson M.J. Molecular screening of Entercoccus virulence determinants and potential for genetic exchanges between food and medical isolates. Appl. Environ. Microbiol. 2001, 67:1628-1635.
6. Gomes B.P.F.A., Ferraz C.C.R., Garrido F.D., Rosalen P.L., Zaia A.A., Teixeira F.B., Souza-Filho F.J. Microbial susceptibility to calcium hidroxide pastes and their vehicles. J. Endod. 2002, 28:758-761.
7. Jaffe C.L., Bennett E., Grimaldi J.R., Mcmahon-Pratt D. Production and characterization of species-specific monoclonal antibodies against Leishmania donovani for immunodiagnostics. J. Immunol. 1984, 133:440-447.
8. Jett B.D., Huycke M.M., Gilmore M.S. Virulence of enterococci. Clin. Microbiol. Rev. 1994, 7:462-478.
9. Kaufman B., Spangberg L., Barry J., Fouad A.F. Enterococcus spp. in endodontically treated teeth with and without periradicular lesions. J. Endod. 2005, 31:851-856.
10. Kristich C.J., Li Y.H., Cvitkovitch D.G., Dunny G.M. Esp-independent biofilm formation by Enterococcus faecalis. J. Bacteriol. 2004, 186:154-163.
11. Koch S., Hunfnagel M., Theilacker C., Huebner J. Enterococcal infections: host response, therapeutic and prophylatic possibilities. Vaccine 2004, 22:822-830.
12. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 333:330-334.
13. Love R.M. Enterococcus faecalis-a mechanism for its role in endodontic failure. Int. Endod. J. 2001, 34:399-405.
14. Malathum K., Singh K.V., Weinstock G.M., Murray B.E. Repetitive sequence-based PCR versus pulsed-field gel electrophoresis for typing of Enterococcus faecalis at the subspecies level. J. Clin. Microbiol. 1998, 36:211-215.
15. Molander A., Reit C., Dahlén G., Kvist T. Microbiological status of root-filled teeth with apical periodontitis. Int. Endod. J. 1998, 31:1-7.
16. Mondino S.S.B., Castro A.C.D., Mondino P.J., Carvalho M.G., Silva K.M., Teixeira L.M. Phenotypic and genotypic characterization of clinical and intestinal enterococci isolated from inpatients and outpatients in two brazilian hospitals. Microbiol. Drug Resist. 2003, 9:167-174.
17. Nakayama J., Kariyama R., Kumon H. Description of a 23.9-kilobase chromosomal deletion containing a region encoding fsr genes which mainly determines the gelatinase-negative phenotype of clinical isolates of Enterococcus faecalis in urine. Appl. Environ. Microbiol. 2002, 68:3152-3155.
18. Namdari H., DelVechio V. Application of PCR for characterization of Enterococci. Clin. Microbiol. Newsletter 1998, 20:91-98.
19. Pacheco A.B.F., Guth B.E., Soares K.C.C., Nishimura L., Almeida D.F., Ferreira L.C.S. Random amplification of polymorphic DNA reveals serotype-specific clonal clusters among enterotoxigenic Escherichia coli strains isolated from humans. J. Clin. Microbiol. 1997, 35:1521-1525.
20. Pinheiro E.T., Gomes B.P.F.A., Ferraz C.C.R., Teixeira F.B., Zaia A.A., Souza-Filho F.J. Evaluation of root canal microorganisms isolated from teeth with endodontic failure and their antimicrobial susceptibility. Oral Microbiol. Immunol. 2003, 18:100-103.
21. Roberts J.C., Singh K.V., Okhuysen P.C., Murray B.E. Molecular epidemiology of the fsr lócus and of gelatinase production among different subsets of Enterococcus faecalis isolates. J. Clin. Microbiol. 2004, 42:2317-2320.
22. Rôças I.N., Siqueira J.F., Santos K.R.N. Association of Enterococcus faecalis with different forms of periradicular diseases. J. Endod. 2004, 30:315-320.
23. Sakamoto M., Siqueira J.F., Rôças I.N., Benno Y. Molecular analysis of the root canal microbiota associated with endodontic treatment failures. Oral Microbiol. Immunol. 2008, 23:275-281.
24. Sassone L., Fidel R., Figueiredo L., Fidel S., Faveri M., Feres M. Evaluation of the microbiota of primary endodontic infections using checkerboard DNA-DNA hybridization. Oral Microbiol. Immunol. 2007, 22:390-397.
25. Sedgley C.M., Lennan S.L., Clewell D.B. Prevalence, phenotype and genotype of oral enterococci. Oral Microbiol. Immunol. 2004, 19:95-101.
26. Sedgley C.M., Lennan S.L., Appelbe O.K. Survival of Enterococcus faecalis in root canals ex vivo. Int. Endod. J. 2005, 38:735-742.
27. Shankar N., Lockatell C.V., Baghdayan A.S., Drachenberg C., Gilmore M.S., Johnson D.E. Role of Enterococcus faecalis surface protein esp in the pathogenesis of ascending urinary tract infection. Infect. Immunol. 2001, 69:4366-4372.
28. Siqueira J.F., Rôças I.N. Polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 2004, 97:85-94.
29. Stepanovic S., Vulkovic D., Hola V., Di Bonaventura G., Djukic S., Cirkovic I., Ruzicka F. Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. APMIS 2007, 115:891-899.
30. Sundqvist G., Figdor D., Persson S., Sjogren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative retreatment. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 1998, 85:86-93.
31. Tendolkar P.M., Baghdayan A.S., Gilmore M.S., Shankar N. Enterococcal surface protein, esp, enhances biofilm formation by Enterococcus faecalis. Infect. Immunol. 2004, 72:6032-6039.
32. Teixeira L.M., Carvalho M.G.S., Merquior V.L.C., Steigerwalt, Teixeira M.G.M., Brenner D.J., Facklam R.R. Recent approaches on the taxonomy of the enterococci and some related microorganisms. Adv. Exp. Med. Biol. 1997, 418:387-391.
33. Teixeira L.M., Facklam R.R. Enterococcus. Manual of Clinical Microbiology 2003, 422-433. ASM Press, Washington, DC. eighth ed. P.R. Murray, E.J. Baron, J.H. Jorgensen, M.A. Pfaller, R.H. Yolken (Eds.).
34. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. U. S. A. 1979, 76:4350-4354.
35. Van Belkum A., Tassios P.T., Dijkshoom L., Haeggman S., Cookson B., Fry N.K., Fussing V., Green J., Feil E., Gemer-Smidt P., Brisse S., Struelens M. Guidelines for the validation and application of typing methods for use in bacterial epidemiology. Clin. Microbiol. Infect. Diseases 2007, 13:1-46.
36. Vivacqua-Gomes N., Gurgel-Filho E.D., Gomes B.P.F.A., Ferraz C.C.R., Zaia A.A., Souza-Filho F.J. Recovery of Enterococcus faecalis after single-or multiple-visit root canal treatments carried out in infected teeth ex vivo. Int. Endod. J. 2005, 38:697-704.
37. Waters C.M., Antiporta M.H., Murray B.E., Dunny G.M. Role of the Enterococcus faecalis gel E protease in determination of cellular chain length, supernatant pheromone levels, and degradation of fibrin and misfolded surface proteins. J. Bacteriol. 2003, 185:3613-3623.
38. Zoletti G.O., Siqueira J.F., Santos K.R.N. Identification of Enterococcus faecalis in root-filled teeth with or without periradicular lesions by culture dependent and-independent approaches. J. Endod. 2006, 32:722-726.