Significant enhancement of (R)-mandelic acid production by relieving substrate inhibition of recombinant nitrilase in toluene-water biphasic system

Journal of Biotechnology

Volumn 152, Issue 1-2, 2011, Pages 24-29

  Zhang, Zhi Jun ^a   Pan, Jiang ^a   Liu, Jun Feng ^a   Xu, Jian He ^a   He, Yu Cai ^b   Liu, You Yan ^c  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b CHANGZHOU UNIVERSITY   (China)

^c GUANGXI UNIVERSITY   (China)

Author keywords

(R) ( ) Mandelic acid; Aqueous organic biphasic system; Enantioselective hydrolysis; Escherichia coli whole cells; High substrate tolerance; Recombinant nitrilase

Indexed keywords

BIPHASIC SYSTEMS; ENANTIOSELECTIVE HYDROLYSIS; HIGH SUBSTRATE TOLERANCE; MANDELIC ACID; RECOMBINANT NITRILASE; WHOLE CELL;

BOTTLES; ESCHERICHIA COLI; HYDROLYSIS; OPTIMIZATION; ORGANIC SOLVENTS; PRODUCTIVITY; TOLUENE;

SUBSTRATES;

MANDELIC ACID; NITRILASE; ORGANIC SOLVENT; RECOMBINANT ENZYME; RECOMBINANT NITRILASE; TOLUENE; UNCLASSIFIED DRUG;

AQUEOUS SOLUTION; ARTICLE; CONTROLLED STUDY; ENANTIOSELECTIVITY; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME SUBSTRATE; ESCHERICHIA COLI; HYDROLYSIS; NONHUMAN; PH; PHYSICAL PHASE; PRIORITY JOURNAL; PROTEIN EXPRESSION; TEMPERATURE;

AMINOHYDROLASES; ESCHERICHIA COLI; MANDELIC ACIDS; RECOMBINANT PROTEINS; TOLUENE; WATER;

ESCHERICHIA COLI;

EID: 79952042445     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.01.013     Document Type: Article

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