Development and initial results of a low cost, disposable, point-of-care testing device for pathogen detection

IEEE Transactions on Biomedical Engineering

Volumn 58, Issue 3 PART 2, 2011, Pages 805-808

  Bearinger, Jane P ^a   Dugan, Lawrence C ^a   Baker, Brian R ^a   Hall, Sara B ^a   Ebert, Katja ^b   Mioulet, Valerie ^b   Madi, Mikidache ^b   King, Donald P ^b  

^a LAWRENCE LIVERMORE NATIONAL LABORATORY   (United States)

^b INSTITUTE FOR ANIMAL HEALTH   (United Kingdom)

Author keywords

Device; disposable; methicillin resistant Staphylococcus aureus (MRSA); point of care testing (POCT); triage; virus

Indexed keywords

DEVICE; DISPOSABLE; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA); POINT-OF-CARE TESTING (POCT); TRIAGE;

BIOMOLECULES; CELLULOSE; COLORIMETRY; NUCLEIC ACIDS; PATHOGENS; POLYMERASE CHAIN REACTION; VETERINARY MEDICINE; VIRUSES;

EQUIPMENT;

BACTERIAL DNA; CELLULOSE; GENOMIC DNA; REAGENT;

ARTICLE; CATTLE; COLOR; COLORIMETRY; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; DISPOSABLE EQUIPMENT; EQUIPMENT DESIGN; FOOT AND MOUTH DISEASE VIRUS; HEATING; INTEGRATED CIRCUIT; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; MEDICAL INSTRUMENTATION; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MICROORGANISM DETECTION; NONHUMAN; NUCLEIC ACID AMPLIFICATION; POINT OF CARE TESTING; POLYMERASE CHAIN REACTION; PROTOTYPE ASSAY DEVICE; PURIFICATION; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION LOOP MEDIATED ISOTHERMAL AMPLIFICATION; SENSITIVITY AND SPECIFICITY; SMEAR; TEST STRIP; TONGUE; VIRUS GENOME; VIRUS RECOMBINANT;

COLORIMETRY; DNA, BACTERIAL; FOOT-AND-MOUTH DISEASE VIRUS; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; POINT-OF-CARE SYSTEMS; RNA, VIRAL; SENSITIVITY AND SPECIFICITY;

EID: 79952018186     PISSN: 00189294     EISSN: None     Source Type: Journal    
DOI: 10.1109/TBME.2010.2089054     Document Type: Article

References (19)

1. Y. Misawa, A. Yoshida, R. Saito, H. Yoshida, K. Okuzumi, N. Ito, M. Okada, K. Moriya, and K. Koike, "Application of loop-mediated isothermal amplification technique to rapid and direct detection of methicillin-resistant Staphylococcus aureus (MRSA) in blood cultures," J. Infect. Chemother, CA, vol. 13, pp. 134-140, Jun. 2007.
2. M. B. Grubman and B. Baxt, "Foot-and-mouth disease," Clin. Microbiol. Rev., vol. 17, pp. 465-493, Apr. 2004.
3. M. C. McClure, S. D. McKay, R. D. Schnabel, and J. F. Taylor, "Assessment of DNA extracted from FTA cards for use on the Illumina iSelect BeadChip," BMC Res. Notes, vol. 2, pp. 107-110, Jun. 2009.
4. S.M. Beckett, S. J. Laughton, L. D. Pozza, G. B. McCowage, G.Marshall, R. J. Cohn, E. Milne, and L. J. Ashton, "Buccal swabs and treated cards: Methodological considerations formolecular epidemiologic studies examining pediatric populations," Am. J. Epidemiol., vol. 167, pp. 1260-1267, May 2008.
5. C. L. Torres, E. A. Vitalis, B. R. Baker, S. N. Gardner, M. W. Torres and J. M. Dzenitis, "LAVA: An open-source approach to designing LAMP (loop mediated isothermal amplification) DNA signatures," BMC Bioinform., submitted for publication.
6. J. P. Dukes, D. P. King, and S. Alexandersen, "Novel reverse transcription loop-mediated isothermal amplification for rapid detection of foot-andmouth disease virus," Arch. Virol., vol. 151, pp. 1093-1106, Jun. 2006.
7. S. M. Reid, K. Ebert, K. Bachanek-Bankowska, C. Batten, A. Sanders, C. Wright, A. E. Shaw, E. D. Ryan, G. H. Hutchings, N. P. Ferris, D. J. Paton, and D. P. King, "Performance of real-time RT-PCR for the detection of foot-and-mouth disease virus during field outbreaks in the United Kingdom in 2007," J. Vet. Diagn. Invest., vol. 21, pp. 321-330, May 2009.
8. M. Goto, E. Honda, A. Ogura, A. Nomoto, and K. Hanaki, "Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue," Biotechniques, vol. 46, pp. 167-172, Mar. 2009.
9. B. O'Shea, "The search for the holy fail(safe)," CBRNeWorld, pp. 52-54, 2008.
10. FTA Cards, GE Healthcare U.K. Ltd., Buckinghamshire, U.K., Data File 51613, Feb. 2010.
11. I. Gustavsson, M. Lindell, E.Wilander, A. Strand, andU.Gyllensten, "Use of FTA card for dry collection, transportation and storage of cervical cell specimen to detect high-risk HPV," J. Clin. Virol., vol. 46, pp. 112-116, Oct. 2009.
12. H. L. Williams, L. Turnbull, S. J. Thomas, A. Murphy, T. Stinear, D. S. Armstrong, and C. B. Whitchurch, "A diagnostic PCR assay for the detection of an Australian epidemic strain of Pseudomonas aeruginosa," Ann. Clin. Microbiol. Antimicrob., vol. 9, pp. 18-25, Jul. 2010.
13. A. Alhassan, O. M. Thekisoe, N. Yokoyama, N. Inoue, M. Y. Motloang, P. A. Mbati, H. Yin, Y. Katayama, T. Anzai, C. Sugimoto, and I. Igarashi, "Development of loop-mediated isothermal amplification (LAMP) method for diagnosis of equine piroplasmosis," Vet. Parasitol., vol. 143, pp. 155-160, Jan. 2007.
14. N. Kobuki, N. Inoue, T. Sakurai, F. Di Cello, D. J. Grab, H. Suzuki, C. Sugimoto, and I. Igarashi, "Loop-mediated isothermal amplification for detection of African trypanosomes," J. Clin. Microbiol., vol. 41, pp. 5517-5524, Dec. 2003.
15. T. C. Hong, Q. L. Mai, D. V. Cuong, M. Parida, H. Minekawa, T. Notomi, F. Hasebe, and K. Morita, "Development and evaluation of a novel loop-mediated isothermal amplification method for rapid detection of severe acute respiratory syndrome coronavirus," J. Clin. Microbiol., vol. 42, pp. 1956-1961, May 2004.
16. H. E. James, K. Ebert, R. McGonigle, S. M. Reid, N. Boonham, J. A. Tomlinson, G. H. Hutchings,M.Denyer, C. A. Oura, J. P.Dukes, and D. P. King, "Detection of African swine fever virus by loop-mediated isothermal amplification," J. Virol. Methods, vol. 164, pp. 68-74, Mar. 2009.
17. K. Nagamine, K. Watanabe, K. Ohtsuka, T. Hase, and T. Notomi, "Loop-mediated isothermal amplification reaction using a nondenatured template," Clin. Chem., vol. 47, pp. 1742-1743, Sep. 2001.
18. J. D. Grab, J. Lonsdale-Eccles, and N. Inoue, "LAMP for tadpoles," Nat. Methods, vol. 2, pp. 635-636, Sep. 2005.
19. X. J. Ma, Y. L. Shu, M. Qin, D. Y. Wang, R. B. Gao, M. Wang, L. Y. Wen, F. Han, S. M. Zhou, X. Zhao, Y. H. Cheng, D. X. Li, and X. P. Dong, "Visual detection of pandemic influenza A H1N1 Virus 2009 by reverse-transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye," J. Virol. Methods, vol. 167, pp. 214-217, Aug. 2010.