Expression, purification, and refolding of biologically active Acinetobacter baumannii OmpA from Escherichia coli inclusion bodies

Protein Expression and Purification

Volumn 77, Issue 1, 2011, Pages 98-103

  McConnell, Michael J ^a   Pachón, Jerónimo ^a  

^a HOSPITAL UNIVERSITARIO VIRGEN DEL ROCÍO   (Spain)

Author keywords

Acinetobacter baumannii; Inclusion bodies; OmpA; Refolding

Indexed keywords

OMPA OUTER MEMBRANE PROTEINS; OUTER MEMBRANE PROTEIN; RECOMBINANT PROTEIN;

ACINETOBACTER BAUMANNII; ARTICLE; BIOSYNTHESIS; CELL INCLUSION; CELL SURVIVAL; CHEMISTRY; ESCHERICHIA COLI; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN REFOLDING; SOLUBILITY; TUMOR CELL LINE;

ACINETOBACTER BAUMANNII; BACTERIAL OUTER MEMBRANE PROTEINS; CELL LINE, TUMOR; CELL SURVIVAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HUMANS; INCLUSION BODIES; PROTEIN REFOLDING; RECOMBINANT PROTEINS; SOLUBILITY;

ACINETOBACTER BAUMANNII; ESCHERICHIA COLI;

EID: 79951942170     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.11.019     Document Type: Article

References (16)

1. L.S. Munoz-Price, R.A. Weinstein, Acinetobacter infection, N. Engl. J. Med. 358 (2008) 1271-1281.
2. J. Vila, J. Pachon, Therapeutic options for Acinetobacter baumannii infections, Expert Opin. Pharmacother. 9 (2008) 587-599.
3. F.S. Taccone, H. Rodriguez-Villalobos, D. De Backer, V. De Moor, J. Deviere, J.L. Vincent, F. Jacobs, Successful treatment of septic shock due to pan-resistant Acinetobacter baumannii using combined antimicrobial therapy including tigecycline, Eur. J. Clin. Microbiol. Infect. Dis. 25 (2006) 257-260. (Pubitemid 43800546)
4. R. Valencia, L.A. Arroyo, M. Conde, J.M. Aldana, M.J. Torres, F. Fernandez-Cuenca, J. Garnacho-Montero, J.M. Cisneros, C. Ortiz, J. Pachon, J. Aznar, Nosocomial outbreak of infection with pan-drug-resistant Acinetobacter baumannii in a tertiary care university hospital, Infect. Control Hosp. Epidemiol. 30 (2009) 257-263.
5. S.G. Smith, V. Mahon, M.A. Lambert, R.P. Fagan, A molecular Swiss army knife: OmpA structure, function and expression, FEMS Microbiol. Lett. 273 (2007) 1-11. (Pubitemid 47024891)
6. A. Gribun, Y. Nitzan, I. Pechatnikov, G. Hershkovits, D.J. Katcoff, Molecular and structural characterization of the HMP-AB gene encoding a pore-forming protein from a clinical isolate of Acinetobacter baumannii, Curr. Microbiol. 47 (2003) 434-443.
7. Y. Nitzan, I. Pechatnikov, D. Bar-El, H. Wexler, Isolation and characterization of heat-modifiable proteins from the outer membrane of Porphyromonas asaccharolytica and Acinetobacter baumannii, Anaerobe 5 (1999) 43-50. (Pubitemid 29391080)
8. T. Surrey, F. Jahnig, Refolding and oriented insertion of a membrane protein into a lipid bilayer, Proc. Natl. Acad. Sci. USA 89 (1992) 7457-7461.
9. D.C. Webb, A.W. Cripps, A method for the purification and refolding of a recombinant form of the nontypeable Haemophilus influenzae P5 outer membrane protein fused to polyhistidine, Protein Expr. Purif. 15 (1999) 1-7.
10. C.H. Choi, E.Y. Lee, Y.C. Lee, T.I. Park, H.J. Kim, S.H. Hyun, S.A. Kim, S.K. Lee, J.C. Lee, Outer membrane protein 38 of Acinetobacter baumannii localizes to the mitochondria and induces apoptosis of epithelial cells, Cell. Microbiol. 7 (2005) 1127-1138.
11. C.H. Choi, J.S. Lee, Y.C. Lee, T.I. Park, J.C. Lee, Acinetobacter baumannii invades epithelial cells and outer membrane protein A mediates interactions with epithelial cells, BMC Microbiol. 8 (2008) 216.
12. J.S. Lee, J.C. Lee, C.M. Lee, I.D. Jung, Y.I. Jeong, E.Y. Seong, H.Y. Chung, Y.M. Park, Outer membrane protein A of Acinetobacter baumannii induces differentiation of CD4+ T cells toward a Th1 polarizing phenotype through the activation of dendritic cells, Biochem. Pharmacol. 74 (2007) 86-97.
13. E. Ofori-Darko, Y. Zavros, G. Rieder, S.A. Tarle, M. Van Antwerp, J.L. Merchant, An OmpA-like protein from Acinetobacter spp. stimulates gastrin and interleukin-8 promoters, Infect. Immun. 68 (2000) 3657-3666.
14. P. Reichelt, C. Schwarz, M. Donzeau, Single step protocol to purify recombinant proteins with low endotoxin contents, Protein Expr. Purif. 46 (2006) 483-488.
15. J.S. Lee, C.H. Choi, J.W. Kim, J.C. Lee, Acinetobacter baumannii outer membrane protein A induces dendritic cell death through mitochondrial targeting, J. Microbiol. 48 (2010) 387-392.
16. S.O. Kwon, Y.S. Gho, J.C. Lee, S.I. Kim, Proteome analysis of outer membrane vesicles from a clinical Acinetobacter baumannii isolate, FEMS Microbiol. Lett. 297 (2009) 150-156.