Triplex PCR for the simultaneous detection of Pseudomonas aeruginosa, Helicobacter hepaticus, and Salmonella typhimurium

Experimental Animals

Volumn 60, Issue 1, 2011, Pages 65-70

  Jeong, Eui Suk ^a   Lee, Kyoung Sun ^a   Heo, Seung Ho ^a,b   Seo, Jin Hee ^a   Choi, Yang Kyu ^a  

^a Konkuk University   (South Korea)

^b University of Ulsan College of Medicine   (South Korea)

Author keywords

H. hepaticus; P. aeruginosa; S. typhimurium; Triplex PCR

Indexed keywords

RIBOSOME RNA; RNA 16S;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIAL GENE; BACTERIAL GENOME; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; CECUM; CONTROLLED STUDY; FECES; HELICOBACTER HEPATICUS; INVA GENE; LIMIT OF DETECTION; LIVER; MOUSE; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PSEUDOMONAS AERUGINOSA; SALMONELLA TYPHIMURIUM; SENSITIVITY AND SPECIFICITY;

ANIMALS; ANIMALS, LABORATORY; BACTERIAL INFECTIONS; BACTERIOLOGICAL TECHNIQUES; HELICOBACTER HEPATICUS; MICE; MICE, INBRED BALB C; POLYMERASE CHAIN REACTION; PSEUDOMONAS AERUGINOSA; SALMONELLA TYPHIMURIUM; SENSITIVITY AND SPECIFICITY;

ANIMALIA; BACTERIA (MICROORGANISMS); HELICOBACTER HEPATICUS; MUS; PSEUDOMONAS AERUGINOSA; SALMONELLA TYPHIMURIUM;

EID: 79951842919     PISSN: 13411357     EISSN: None     Source Type: Journal    
DOI: 10.1538/expanim.60.65     Document Type: Article

References (17)

1. de Freitas, C.G., Santana, A.P., da Silva, P.H., Gonçalves, V.S., Barros, M. de A., Torres, F.A., Murata, L.S., and Perecmanis, S. 2010. PCR multiplex for detection of Salmonella Enteritidis, Typhi and Typhimurium and occurrence in poultry meat. Int. J. Food Microbiol. 139: 15-22.
2. Fox, J.G. and Lee, A. 1997. The role of Helicobacter species in newly recognized gastrointestinal tract diseases of animals. Lab. Anim. Sci. 47: 222-255.
3. Kim, S.H., Kim, S.H., Lee, S.W., Kang, Y.H., and Lee, B.K. 2005. Rapid serological identification for monophasic Salmonella serovars with a hin gene-specific polymerase chain reaction. J. Bacteriol. Virol. 35: 291-297.
4. Lee, K.M., McReynolds, J.L., Fuller, C.C., Jones, B., Herrman, T.J., Byrd, J.A., and Runyon, M. 2008. Investigation and characterization of the frozen feeder rodent industry in Texas following a multi-state Salmonella Typhimurium outbreak associated with frozen vacuum-packed rodents. Zoonoses Public Health 55: 488-496.
5. Li, Y. and Mustapha, A. 2004. Simultaneous detection of Escherichia coli O157:H7, Salmonella, and Shigella in apple cider and produce by a multiplex PCR. J. Food Prot. 67: 27-33.
6. Manafi, A., Kohanteb, J., Mehrabani, D., Japoni, A., Amini, M., Naghmachi, M., Zaghi, A.H., and Khalili, N. 2009. Active immunization using exotoxin A confers protection against Pseudomonas aeruginosa infection in a mouse burn model. BMC Microbiol. 9: 23.
7. Millar, B.C., Xu, J., and Moore, J.E. 2007 Molecular diagnostics of medically important bacterial infections. Curr. Issues Mol. Biol. 9: 21-39.
8. Nastasi, A., Mammina, C., and Villafrate, M.R. 1993. Epidemiology of Salmonella typhimurium: ribosomal DNA analysis of strains from human and animal sources. Epidemiol. Infect. 110: 553-565.
9. Park, S.H., Kim, H.J., Cho, W.H., Kim, J.H., Oh, M.H., Kim, S.H., Lee, B.K., Ricke, S.C., and Kim, H.Y. 2009. Identification of Salmonella enterica subspecies I, Salmonella enterica serovars Typhimurium, Enteritidis and Typhi using multiplex PCR. FEMS Microbiol. Lett. 301: 137-146.
10. Popoff, M.Y., Bockemühl, J., and Gheesling, L.L. 2003. Supplement 2001 (no. 45) to the Kauffmann-White scheme. Res. Microbiol. 154: 173-184.
11. Shames, B., Fox, J.G., Dewhirst, F., Yan, L., Shen, Z., and Taylor, N.S. 1995. Identification of widespread Helicobacter hepaticus infection in feces in commercial mouse colonies by culture and PCR assay. J. Clin. Microbiol. 33: 2968-2972.
12. Urano, T., Noguchi, K., Jiang, G., and Tsukumi, K. 1995. Survey of Pseudomonas aeruginosa contamination in human beings and laboratory animals. Exp. Anim. 44: 233-239.
13. Waggie, K., Kagiyam, N., Allen, A.M., and Nomura, T. 1994. Manual of Microbiologic Monitoring of Laboratory Animals, 2nd ed., NIH Publication, Mayland.
14. Ward, J.M., Anver, M.R., Haines, D.C., Melhorn, J.M., Gorelick, P., Yan, L., and Fox, J.G. 1996. Inflammatory large bowel disease in immunodeficient mice naturally infected with Helicobacter hepaticus. Lab. Anim. Sci. 46: 15-20.
15. Ward, J.M., Fox, J.G., Anver, M.R., Haines, D.C., George, C.V., Collins, M.J., Gorelick, P.L., Nagashima, K., Gonda, M.A., Gilden, R.V., Tully, J.G., Russell, R.J., Benveniste, R.E., Paster, B.J., Dewhirst, F.E., Donovan, J.C., Anderson, L.M., and Rice, J.M. 1994. Chronic active hepatitis and associated liver tumors in mice caused by a persistent bacterial infection with a novel Helicobacter species. J. Natl. Cancer Inst. 86: 1222-1227.
16. Won, Y.S., Jeong, E.S, Park, H.J., Lee, C.H., Nam, K.H., Kim, H.C., Hyun, B.H., Lee, S.K., and Choi, Y.K. 2006. Microbiological contamination of laboratory mice and rats in Korea from 1999 to 2003. Exp. Anim. 55: 11-16.
17. Yuan, Y., Xu, W., Zhai, Z., Shi, H., Luo, Y., Chen, Z., and Huang, K. 2009. Universal primer-multiplex PCR approach for simultaneous detection of Escherichia coli, Listeria monocytogenes, and Salmonella spp. in food samples. J. Food Sci. 74: M446-452.