In vivo transfection of developmentally competent Brugia malayi infective larvae

International Journal for Parasitology

Volumn 41, Issue 3, 2011, Pages 355-362

  Xu, Shulin ^a   Liu, Canhui ^a   Tzertzinis, George ^b   Ghedin, Elodie ^c   Evans, Christopher C ^d   Kaplan, Ray ^d   Unnasch, Thomas R ^a  

^a UNIVERSITY OF SOUTH FLORIDA   (United States)

^b New England Biolabs   (United States)

^c UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^d UNIVERSITY OF GEORGIA   (United States)

Author keywords

Filariasis; Gaussia; HSP70; Luciferase; Transfection; Transgenesis

Indexed keywords

HEAT SHOCK PROTEIN 70; LUCIFERASE; MESSENGER RNA; RNA;

DNA; EFFICIENCY MEASUREMENT; EMBRYO; FILARIASIS; IN SITU TEST; NEMATODE; PARASITE;

3' UNTRANSLATED REGION; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BRUGIA MALAYI; BRUGIAN FILARIASIS; CONTROLLED STUDY; EMBRYO; FEMALE; GENETIC TRANSFECTION; GERBIL; IN VIVO STUDY; MICROFILARIA (NEMATODE LARVA); MOLTING; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE TRANSMISSION; PARASITE VIRULENCE; PERITONEAL CAVITY; PROMOTER REGION; REPORTER GENE; RNA SPLICING; TRANSGENICS;

ANIMALS; BIOLISTICS; BRUGIA MALAYI; DNA, HELMINTH; GENE EXPRESSION REGULATION; GENE TRANSFER TECHNIQUES; GENES, REPORTER; LARVA; LUCIFERASES; MICROFILARIA; MOLECULAR SEQUENCE DATA; RNA, MESSENGER; SEQUENCE ANALYSIS, DNA; TRANSFECTION;

BRUGIA MALAYI; GERBILLINAE;

EID: 79951814043     PISSN: 00207519     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijpara.2010.10.005     Document Type: Article

References (30)

1. Abraham D., Lange A.M., Yutanawiboonchai W., Trpis M., Dickerson J.W., Swenson B., Eberhard M.L. Survival and development of larval Onchocerca volvulus in diffusion chambers implanted in primate and rodent hosts. J. Parasitol. 1993, 79:571-582.
2. African Programme for Onchocerciasis Control, 2005. Final communiqué of the 11th session of the Joint Action Forum (JAF) of African Programme for Onchocerciasis Control (APOC), Paris, France 6-9 December 2005. APOC, Ouagadougou, Burkina Faso.
3. Bradford M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72:248-254.
4. Cheng G., Davis R.E. An improved and secreted luciferase reporter for schistosomes. Mol. Biochem. Parasitol. 2007, 155:167-171.
5. de Oliveira A.D., Katholi C.R., Unnasch T.R. Characterization of the promoter of the Brugia malayi 12kDa small subunit ribosomal protein (RPS12) gene. Int. J. Parasitol. 2008, 38:1111-1119.
6. Denham D.A., Fletcher C. The cat infected with Brugia pahangi as a model of human filariasis. Ciba Found. Symp. 1987, 127:225-235.
7. Ghedin E., Wang S., Spiro D., Caler E., Zhao Q., Crabtree J., Allen J.E., Delcher A.L., Guiliano D.B., Miranda-Saavedra D., Angiuoli S.V., Creasy T., Amedeo P., Haas B., El-Sayed N.M., Wortman J.R., Feldblyum T., Tallon L., Schatz M., Shumway M., Koo H., Salzberg S.L., Schobel S., Pertea M., Pop M., White O., Barton G.J., Carlow C.K., Crawford M.J., Daub J., Dimmic M.W., Estes C.F., Foster J.M., Ganatra M., Gregory W.F., Johnson N.M., Jin J., Komuniecki R., Korf I., Kumar S., Laney S., Li B.W., Li W., Lindblom T.H., Lustigman S., Ma D., Maina C.V., Martin D.M., McCarter J.P., McReynolds L., Mitreva M., Nutman T.B., Parkinson J., Peregrin-Alvarez J.M., Poole C., Ren Q., Saunders L., Sluder A.E., Smith K., Stanke M., Unnasch T.R., Ware J., Wei A.D., Weil G., Williams D.J., Zhang Y., Williams S.A., Fraser-Liggett C., Slatko B., Blaxter M.L., Scott A.L. Draft genome of the filarial nematode parasite Brugia malayi. Science 2007, 317:1756-1760.
8. Higazi T.B., Merriweather A., Shu L., Davis R., Unnasch T.R. Brugia malayi: transient transfection by microinjection and particle bombardment. Exp. Parasitol. 2002, 100:95-102.
9. Higazi T.B., Unnasch T.R. Intron encoded sequences necessary for trans splicing in transiently transfected Brugia malayi. Mol. Biochem. Parasitol. 2004, 137:181-184.
10. Higazi T.B., DeOliveira A., Katholi C.R., Shu L., Barchue J., Lisanby M., Unnasch T.R. Identification of elements essential for transcription in Brugia malayi promoters. J. Mol. Biol. 2005, 353:1-13.
11. Katholi C.R., Toé L., Merriweather A., Unnasch T.R. Determining the prevalence of Onchocerca volvulus infection in vector populations by PCR screening of pools of black flies. J. Infect. Dis. 1995, 172:1414-1417.
12. Kines K.J., Mann V.H., Morales M.E., Shelby B.D., Kalinna B.H., Gobert G.N., Chirgwin S.R., Brindley P.J. Transduction of Schistosoma mansoni by vesicular stomatitis virus glycoprotein-pseudotyped Moloney murine leukemia retrovirus. Exp. Parasitol. 2006, 112:209-220.
13. Kines K.J., Morales M.E., Mann V.H., Gobert G.N., Brindley P.J. Integration of reporter transgenes into Schistosoma mansoni chromosomes mediated by pseudotyped murine leukemia virus. FASEB J. 2008, 22:2936-2948.
14. Li X., Massey H.C., Nolan T.J., Schad G.A., Kraus K., Sundaram M., Lok J.B. Successful transgenesis of the parasitic nematode Strongyloides stercoralis requires endogenous non-coding control elements. Int. J. Parasitol. 2006, 36:671-679.
15. Liu C., de Oliveira A., Higazi T.B., Ghedin E., DePasse J., Unnasch T.R. Sequences necessary for trans-splicing in transiently transfected Brugia malayi. Mol. Biochem. Parasitol. 2007, 156:62-73.
16. Liu C., Chauhan C., Katholi C.R., Unnasch T.R. The splice leader addition domain represents an essential conserved motif for heterologous gene expression in B. malayi. Mol. Biochem. Parasitol. 2009, 166:15-21.
17. Liu C., Chauhan C., Unnasch T.R. The role of local secondary structure in the function of the trans splicing motif of Brugia malayi. Mol. Biochem. Parasitol. 2010, 169:115-119.
18. Lok J.B., Massey H.C. Transgene expression in Strongyloides stercoralis following gonadal microinjection of DNA constructs. Mol. Biochem. Parasitol. 2002, 119:279-284.
19. Mak J.W., Choong M.F., Lam P.L., Suresh K. Experimental infection of the leaf-monkeys, Presbytis cristata and Presbytis melalophos with subperiodic Brugia malayi. Acta Trop. 1990, 47:223-226.
20. Mello C.C., Kramer J.M., Stinchcomb D., Ambros V. Efficient gene transfer in C. elegans: extrachromosomal maintenance and integration of transforming sequences. EMBO J. 1991, 10:3959-3970.
21. Morales M.E., Mann V.H., Kines K.J., Gobert G.N., Fraser M.J., Kalinna B.H., Correnti J.M., Pearce E.J., Brindley P.J. PiggyBac transposon mediated transgenesis of the human blood fluke, Schistosoma mansoni. FASEB J. 2007, 21:3479-3489.
22. Nelson F.K., Greiner D.L., Shultz L.D., Rajan T.V. The immunodeficient SCID mouse as a model for human lymphatic filariasis. J. Exp. Med. 1991, 173:659-663.
23. Rajan T.V., Paciorkowski N., Kalajzic I., McGuiness C. Ascorbic acid is a requirement for the morphogenesis of the human filarial parasite Brugia malayi. J. Parasitol. 2003, 89:868-870.
24. Schlager B., Wang X., Braach G., Sommer R.J. Molecular cloning of a dominant roller mutant and establishment of DNA-mediated transformation in the nematode Pristionchus pacificus. Genesis 2009, 47:300-304.
25. Shu L., Katholi C., Higazi T., Unnasch T.R. Analysis of the Brugia malayi HSP70 promoter using a homologous transient transfection system. Mol. Biochem. Parasitol. 2003, 128:67-75.
26. Tannous B.A., Kim D.E., Fernandez J.L., Weissleder R., Breakefield X.O. Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo. Mol. Ther. 2005, 11:435-443.
27. Tzertzinis G., Egana A.L., Palli S.R., Robinson-Rechavi M., Gissendanner C.R., Liu C., Unnasch T.R., Maina C.V. Molecular evidence for a functional ecdysone signaling system in Brugia malayi. PLOS NTD 2010, 4:e625.
28. Warren K.S., Mahmoud A.A.F. Tropical and Geographical Medicine 1985, Mc Graw-Hill, New York.
29. Weil G.J., Li B.W., Liftis F., Chandrashekar R. Brugia malayi: antibody responses to larval antigens in infected and immunized jirds. Exp. Parasitol. 1992, 74:315-323.
30. Wurdinger T., Badr C., Pike L., de Kleine R., Weissleder R., Breakefield X.O., Tannous B.A. A secreted luciferase for ex vivo monitoring of in vivo processes. Nat. Methods 2008, 5:171-173.