SOE-LRed: A simple and time-efficient method to localize genes with point mutations onto the Escherichia coli chromosome

Journal of Microbiological Methods

Volumn 84, Issue 3, 2011, Pages 479-481

  Benson, Ryan W ^a   Cafarelli, Tiziana M ^a   Godoy, Veronica G ^a  

^a NORTHEASTERN UNIVERSITY   (United States)

Author keywords

DinB; E. coli; Lambda Red; Point mutation; SOE PCR; Transformation

Indexed keywords

LAMBDA RED RECOMBINASE; RECOMBINASE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIAL STRAIN; DNA RECOMBINATION; DNA SEQUENCE; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE EXPRESSION; GENE FUSION; GENE LOCATION; HOMOLOGOUS RECOMBINATION; MOLECULAR CLONING; MUTATIONAL ANALYSIS; NONHUMAN; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; SPLICING BY OVERLAP EXTENSION; WILD TYPE;

ARTIFICIAL GENE FUSION; CHROMOSOME MAPPING; CHROMOSOMES, BACTERIAL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENES, BACTERIAL; GENETICS, MICROBIAL; MOLECULAR BIOLOGY; MUTANT PROTEINS; POINT MUTATION; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC;

ESCHERICHIA COLI;

EID: 79951813219     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2010.12.020     Document Type: Article

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