Intergenotypic chimeric hepatitis E viruses (HEVs) with the genotype 4 human HEV capsid gene in the backbone of genotype 3 swine HEV are infectious in pigs

Virus Research

Volumn 156, Issue 1-2, 2011, Pages 141-146

  Feagins, Alicia R ^a   Córdoba, Laura ^a   Sanford, Brent J ^a   Dryman, Barbara A ^a   Huang, Yao Wei ^a   LeRoith, Tanya ^a   Emerson, Suzanne U ^b   Meng, Xiang Jin ^a  

^a CENTER FOR MOLECULAR MEDICINE AND INFECTIOUS DISEASES   (United States)

^b NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

Chimeric viruses; Cross species infection; Hepatitis E virus (HEV); Host range; Pigs

Indexed keywords

CAPSID PROTEIN; ORF2 PROTEIN; UNCLASSIFIED DRUG;

3' NONCODING REGION; 5' JUNCTION REGION; ARTICLE; CHIMERA; CONTROLLED STUDY; CROSS INFECTION; GENE STRUCTURE; GENOMICS; GENOTYPE; HEPATITIS E; HEPATITIS E VIRUS; HUMAN; HUMAN CELL; IN VITRO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; SWINE; VIRUS CAPSID; VIRUS GENE; VIRUS INFECTIVITY; VIRUS REPLICATION;

ANIMALS; CAPSID PROTEINS; CELL LINE, TUMOR; GENE ORDER; GENOTYPE; HEP G2 CELLS; HEPATITIS E; HEPATITIS E VIRUS; HUMANS; IMMUNOGLOBULIN G; SWINE; SWINE DISEASES; VIREMIA; VIRUS SHEDDING;

HEPATITIS E VIRUS; MIRIDAE; SUIDAE;

EID: 79951809577     PISSN: 01681702     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.virusres.2010.12.011     Document Type: Article

References (34)

1. Agrawal S., Gupta D., Panda S.K. The 3′ end of hepatitis E virus (HEV) genome binds specifically to the viral RNA-dependent RNA polymerase (RdRp). Virology 2001, 282:87-101.
2. Arankalle V.A., Chobe L.P., Chadha M.S. Type-IV Indian swine HEV infects rhesus monkeys. J. Viral Hepat. 2006, 13:742-745.
3. Chandra V., Kar-Roy A., Kumari S., Mayor S., Jameel S. The hepatitis E virus ORF3 protein modulates epidermal growth factor receptor trafficking, STAT3 translocation, and the acute-phase response. J. Virol. 2008, 82:7100-7110.
4. Emerson S.U., Anderson D., Arankalle A., Meng X.J., Purdy M., Schlauder G.G., Tsarev S.A. Hepevirus. Virus Taxonomy, VIIIth Report of the ICTV 2004, 851-855. Elsevier/Academic Press, London. C.M. Fauquet, M.A. Mayo, J. Maniloff, U. Desselberger, L.A. Ball (Eds.).
5. Emerson S.U., Nguyen H., Graff J., Stephany D.A., Brockington A., Purcell R.H. In vitro replication of hepatitis E virus (HEV) genomes and of an HEV replicon expressing green fluorescent protein. J. Virol. 2004, 78:4838-4846.
6. Emerson S.U., Nguyen H., Torian U., Purcell R.H. ORF3 protein of hepatitis E virus is not required for replication, virion assembly, or infection of hepatoma cells in vitro. J. Virol. 2006, 80:10457-10464.
7. Emerson S.U., Nguyen H.T., Torian U., Burke D., Engle R., Purcell R.H. Release of genotype 1 hepatitis E virus from cultured hepatoma and polarized intestinal cells depends on ORF3 protein and requires an intact PXXP motif. J. Virol. 2010, 84:9059-9069.
8. Emerson S.U., Zhang M., Meng X.J., Nguyen H., St Claire M., Govindarajan S., Huang Y.K., Purcell R.H. Recombinant hepatitis E virus genomes infectious for primates: importance of capping and discovery of a cis-reactive element. Proc. Natl. Acad. Sci. U.S.A. 2001, 98:15270-15275.
9. Feagins A.R., Opriessnig T., Huang Y.W., Halbur P.G., Meng X.J. Cross-species infection of specific-pathogen-free pigs by a genotype 4 strain of human hepatitis E virus. J. Med. Virol. 2008, 80:1379-1386.
10. Graff J., Nguyen H., Yu C., Elkins W.R., St Claire M., Purcell R.H., Emerson S.U. The open reading frame 3 gene of hepatitis E virus contains a cis-reactive element and encodes a protein required for infection of macaques. J. Virol. 2005, 79:6680-6689.
11. Graff J., Torian U., Nguyen H., Emerson S.U. A bicistronic subgenomic mRNA encodes both the ORF2 and ORF3 proteins of hepatitis E virus. J. Virol. 2006, 80:5919-5926.
12. Guu T.S., Liu Z., Ye Q., Mata D.A., Li K., Yin C., Zhang J., Tao Y.J. Structure of the hepatitis E virus-like particle suggests mechanisms for virus assembly and receptor binding. Proc. Natl. Acad. Sci. U.S.A. 2009, 106:12992-12997.
13. He S., Miao J., Zheng Z., Wu T., Xie M., Tang M., Zhang J., Ng M.H., Xia N. Putative receptor-binding sites of hepatitis E virus. J. Gen. Virol. 2008, 89:245-249.
14. Huang F.F., Haqshenas G., Guenette D.K., Halbur P.G., Schommer S.K., Pierson F.W., Toth T.E., Meng X.J. Detection by reverse transcription-PCR and genetic characterization of field isolates of swine hepatitis E virus from pigs in different geographic regions of the United States. J. Clin. Microbiol. 2002, 40:1326-1332.
15. Huang Y.W., Haqshenas G., Kasorndorkbua C., Halbur P.G., Emerson S.U., Meng X.J. Capped RNA transcripts of full-length cDNA clones of swine hepatitis E virus are replication competent when transfected into Huh7 cells and infectious when intrahepatically inoculated into pigs. J. Virol. 2005, 79:1552-1558.
16. Huang Y.W., Opriessnig T., Halbur P.G., Meng X.J. Initiation at the third in-frame AUG codon of open reading frame 3 of the hepatitis E virus is essential for viral infectivity in vivo. J. Virol. 2007, 81:3018-3026.
17. Kalia M., Chandra V., Rahman S.A., Sehgal D., Jameel S. Heparan sulfate proteoglycans are required for cellular binding of the hepatitis E virus ORF2 capsid protein and for viral infection. J. Virol. 2009, 83:12714-12724.
18. Kar-Roy A., Korkaya H., Oberoi R., Lal S.K., Jameel S. The hepatitis E virus open reading frame 3 protein activates ERK through binding and inhibition of the MAPK phosphatase. J. Biol. Chem. 2004, 279:28345-28357.
19. Kasorndorkbua C., Guenette D.K., Huang F.F., Thomas P.J., Meng X.J., Halbur P.G. Routes of transmission of swine hepatitis E virus in pigs. J. Clin. Microbiol. 2004, 42:5047-5052.
20. Koonin E.V., Gorbalenya A.E., Purdy M.A., Rozanov M.N., Reyes G.R., Bradley D.W. Computer-assisted assignment of functional domains in the nonstructural polyprotein of hepatitis E virus: delineation of an additional group of positive-strand RNA plant and animal viruses. Proc. Natl. Acad. Sci. U.S.A. 1992, 89:8259-8263.
21. Meng X.J. Hepatitis E virus: animal reservoirs and zoonotic risk. Vet. Microbiol. 2010, 140:256-265.
22. Meng X.J., Halbur P.G., Haynes J.S., Tsareva T.S., Bruna J.D., Royer R.L., Purcell R.H., Emerson S.U. Experimental infection of pigs with the newly identified swine hepatitis E virus (swine HEV), but not with human strains of HEV. Arch. Virol. 1998, 143:1405-1415.
23. Meng X.J., Halbur P.G., Shapiro M.S., Govindarajan S., Bruna J.D., Mushahwar I.K., Purcell R.H., Emerson S.U. Genetic and experimental evidence for cross-species infection by swine hepatitis E virus. J. Virol. 1998, 72:9714-9721.
24. Meng X.J., Purcell R.H., Halbur P.G., Lehman J.R., Webb D.M., Tsareva T.S., Haynes J.S., Thacker B.J., Emerson S.U. A novel virus in swine is closely related to the human hepatitis E virus. Proc. Natl. Acad. Sci. U.S.A. 1997, 94:9860-9865.
25. Moin S.M., Panteva M., Jameel S. The hepatitis E virus Orf3 protein protects cells from mitochondrial depolarization and death. J. Biol. Chem. 2007, 282:21124-21133.
26. Panda S.K., Ansari I.H., Durgapal H., Agrawal S., Jameel S. The in vitro-synthesized RNA from a cDNA clone of hepatitis E virus is infectious. J. Virol. 2000, 74:2430-2437.
27. Pudupakam R.S., Huang Y.W., Opriessnig T., Halbur P.G., Pierson F.W., Meng X.J. Deletions of the hypervariable region (HVR) in open reading frame 1 of hepatitis E virus do not abolish virus infectivity: evidence for attenuation of HVR deletion mutants in vivo. J. Virol. 2009, 83:384-395.
28. Riddell M.A., Li F., Anderson D.A. Identification of immunodominant and conformational epitopes in the capsid protein of hepatitis E virus by using monoclonal antibodies. J. Virol. 2000, 74:8011-8017.
29. Surjit M., Jameel S., Lal S.K. The ORF2 protein of hepatitis E virus binds the 5' region of viral RNA. J. Virol. 2004, 78:320-328.
30. Tsarev S.A., Tsareva T.S., Emerson S.U., Yarbough P.O., Legters L.J., Moskal T., Purcell R.H. Infectivity titration of a prototype strain of hepatitis E virus in cynomolgus monkeys. J. Med. Virol. 1994, 43:135-142.
31. Tyagi S., Korkaya H., Zafrullah M., Jameel S., Lal S.K. The phosphorylated form of the ORF3 protein of hepatitis E virus interacts with its non-glycosylated form of the major capsid protein, ORF2. J. Biol. Chem. 2002, 277:22759-22767.
32. Wu J.C., Chen C.M., Chiang T.Y., Tsai W.H., Jeng W.J., Sheen I.J., Lin C.C., Meng X.J. Spread of hepatitis E virus among different-aged pigs: two-year survey in Taiwan. J. Med. Virol. 2002, 66:488-492.
33. Xia H., Wahlberg N., Belak S., Meng X.J., Liu L. The emergence of genotypes 3 and 4 hepatitis E virus in swine and humans: a phylogenetic perspective. Arch. Virol. 2011, 156:121-124.
34. Yamada K., Takahashi M., Hoshino Y., Takahashi H., Ichiyama K., Nagashima S., Tanaka T., Okamoto H. ORF3 protein of hepatitis E virus is essential for virion release from infected cells. J. Gen. Virol. 2009, 90:1880-1891.