Capped RNA transcripts of full-length cDNA clones of swine hepatitis E virus are replication competent when transfected into Huh7 cells and infectious when intrahepatically inoculated into pigs

Journal of Virology

Volumn 79, Issue 3, 2005, Pages 1552-1558

  Huang, Y W ^a   Haqshenas, G ^a,d   Kasorndorkbua, C ^b   Halbur, P G ^b   Emerson, S U ^c   Meng, X J ^a  

^a CENTER FOR MOLECULAR MEDICINE AND INFECTIOUS DISEASES   (United States)

^b IOWA STATE UNIVERSITY   (United States)

^c NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^d NATIONAL INSTITUTE OF GENETIC ENGINEERING AND BIOTECHNOLOGY   (Iran)

Author keywords

[No Author keywords available]

Indexed keywords

CAPPED RNA; COMPLEMENTARY DNA; PROTEIN ORF2; PROTEIN ORF3; UNCLASSIFIED DRUG; VIRUS PROTEIN; VIRUS RNA;

ARTICLE; GENETIC TRANSFECTION; HEPATITIS E VIRUS; HUMAN; HUMAN CELL; INOCULATION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA TRANSCRIPTION; SEROCONVERSION; SITE DIRECTED MUTAGENESIS; SWINE; SWINE HEPATITIS E VIRUS 1; SWINE HEPATITIS E VIRUS 2; SWINE HEPATITIS E VIRUS 3; VIREMIA; VIRUS CAPSID; VIRUS GENOME; VIRUS ISOLATION; VIRUS REPLICATION; VIRUS SHEDDING; ANIMAL; ANIMAL DISEASE; ANIMAL HEPATITIS; DNA SEQUENCE; GENETIC TRANSCRIPTION; GENETICS; HEPATITIS E; LIVER; METABOLISM; MOLECULAR GENETICS; PATHOGENICITY; PHYSIOLOGY; SWINE DISEASE; TUMOR CELL LINE; VIROLOGY;

ANIMALS; CELL LINE, TUMOR; CLONING, MOLECULAR; DNA, COMPLEMENTARY; HEPATITIS E; HEPATITIS E VIRUS; HEPATITIS, VIRAL, ANIMAL; HUMANS; LIVER; MOLECULAR SEQUENCE DATA; RNA CAPS; RNA, VIRAL; SEQUENCE ANALYSIS, DNA; SWINE; SWINE DISEASES; TRANSCRIPTION, GENETIC; TRANSFECTION; VIRUS REPLICATION;

EID: 13744251265     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.79.3.1552-1558.2005     Document Type: Article

References (36)

1. Arankalle, V. A., M. V. Joshi, A. M. Kulkarni, S. S. Gandhe, L. P. Chobe, S. S. Rautmare, A. C. Mishra, and V. S. Padbidri. 2001. Prevalence of anti-hepatitis E virus antibodies in different Indian animal species. J. Viral Hepat. 8:223-227.
2. Boyer, J. C., and A. L. Haenni. 1994. Infectious transcripts and cDNA clones of RNA viruses. Virology 198:415-426.
3. Dietzschold, B., W. H. Wunner, T. J. Wiktor, A. D. Lopes, M. Lafon, C. L. Smith, and H. Koprowski. 1983. Characterization of an antigenic determinant of the glycoprotein that correlates with pathogenicity of rabies virus. Proc. Natl. Acad. Sci. USA 80:70-74.
4. Drobeniuc, J., M. O. Favorov, C. N. Shapiro, B. P. Bell, E. E. Mast, A. Dadu, D. Culver, P. Iarovoi, B. H. Robertson, and H. S. Margolis. 2001. Hepatitis E virus antibody prevalence among persons who work with swine. J. Infect. Dis. 184:1594-1597.
5. Emerson, S. U., and R. H. Purcell. 2003. Hepatitis E virus. Rev. Med. Virol. 13:145-154.
6. Emerson, S. U., H. Nguyen, J. Graff, D. A. Stephany, A. Brockington, and R. H. Purcell. 2004. In vitro replication of hepatitis E virus (HEV) genomes and of an HEV replicon expressing green fluorescent protein. J. Virol. 78: 4838-4846.
7. Emerson, S. U., M. Zhang, X. J. Meng, H. Nguyen, M. St. Claire, S. Govindarajan, Y. K. Huang, and R. H. Purcell. 2001. Recombinant hepatitis E virus genomes infectious for primates: importance of capping and discovery of a cis-reactive element. Proc. Natl. Acad. Sci. USA 98:15270-15275.
8. Emerson, S. U., D. Anderson, A. Arankalle, X. J. Meng, M. Purdy, G. G. Schlauder, and S. A. Tsarev. 2004. Hepevirus, p. 851-855. In C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger, and L. A. Ball (ed.), Virus taxonomy. VIIIth Report of the ICTV. Elsevier/Academic Press, London, United Kingdom.
9. Erker, J. C., S. M. Desai, G. G. Schlauder, G. J. Dawson, and I. K. Mushahwar. 1999. A hepatitis E virus variant from the United States: molecular characterization and transmission in cynomolgus macaques. J. Gen. Virol. 80:681-690.
10. Favorov, M. O., M. Y. Kosoy, S. A. Tsarev, J. E. Childs, and H. S. Margolis. 2000. Prevalence of antibody to hepatitis E virus among rodents in the United States. J. Infect. Dis. 181:449-455.
11. Halbur, P. G., C. Kasorndorkbua, C. Gilbert, D. Guenette, M. B. Potters, R. H. Purcell, S. U. Emerson, T. E. Toth, and X. J. Meng. 2001. Comparative pathogenesis of infection of pigs with hepatitis E viruses recovered from a pig and a human. J. Clin. Microbiol. 39:918-923.
12. Hamid, S. S., S. M. Jafri, H. Khan, H. Shah, Z. Abbas, and H. Fields. 1996. Fulminant hepatic failure in pregnant women: acute fatty liver or acute viral hepatitis? J. Hepatol. 25:20-27.
13. Haqshenas, G., and X. J. Meng. 2001, Determination of the nucleotide sequences at the extreme 5′ and 3′ ends of swine hepatitis E virus genome. Arch. Virol. 146:2461-2467.
14. Hussaini, S. H., S. J. Skidmore, P. Richardson, L. M. Sherratt, B. T. Cooper, and J. G. O'Grady. 1997. Severe hepatitis E infection during pregnancy. J. Viral Hepat. 4:51-54.
15. Kabrane-Lazizi, Y., J. B. Fine, J. Elm, G. E. Glass, H. Higa, A. Diwan, C. J. Gibbs, Jr., X. J. Meng, S. U. Emerson, and R. H. Purcell. 1999. Evidence for widespread infection of wild rats with hepatitis E virus in the United States. Am. J. Trap. Med. Hyg. 61:331-335.
16. Kabrane-Lazizi, Y., M. Zhang, R. H. Purcell, K. D. Miller, R. T. Davey, and S. U. Emerson. 2001. Acute hepatitis caused by a novel strain of hepatitis E virus most closely related to United States strains. J. Gen. Virol. 82:1687-1693.
17. Kasorndorkbua, C., P. G. Halbur, D. K. Guenette, T. E. Toth, and X. J. Meng. 2002. Use of a swine bioassay and an RT-PCR assay to assess the risk of transmission of swine hepatitis E virus in pigs. J. Virol. Methods 101:71-78.
18. Koonin, E. V., A. E. Gorbalenya, M. A. Purdy, M. N. Rozanov, G. R. Reyes, and D. W. Bradley. 1992. Computer-assisted assignment of functional domains in the nonstructural polyprotein of hepatitis E virus: delineation of an additional group of positive-strand RNA plant and animal viruses. Proc. Natl. Acad. Sci. USA 89:8259-8263.
19. Mast, E. E., I. K. Kuramoto, M. O. Favorov, V. R. Schoening, B. T. Burkholder, C. N. Shapiro, and P. V. Holland. 1997. Prevalence of and risk factors for antibody to hepatitis E virus seroreactivity among blood donors in Northern California. J. Infect. Dis. 176:34-40.
20. Meng, J., X. Dai, J. C. Chang, E. Lopareva, J. Pillot, H. A. Fields, and Y. E. Khudyakov. 2001. Identification and characterization of the neutralization epitope(s) of the hepatitis E virus. Virology 288:203-211.
21. Meng, X. J. 2000. Novel strains of hepatitis E virus identified from humans and other animal species: is hepatitis E a zoonosis? J. Hepatol. 33:842-845.
22. Meng, X. J. 2003. Swine hepatitis E virus: cross-species infection and risk in xenotransplantation. Curr. Top. Microbiol. Immunol. 278:185-216.
23. Meng, X. J., P. G. Halbur, J. S. Haynes, T. S. Tsareva, J. D. Bruna, R. L. Royer, R. H. Purcell, and S. U. Emerson. 1998. Experimental infection of pigs with the newly identified swine hepatitis E virus (swine HEV), but not with human strains of HEV. Arch. Virol. 143:1405-1415.
24. Meng, X. J., P. G. Halbur, M. S. Shapiro, S. Govindarajan, J. D. Bruna, I. K. Mushahwar, R. H. Purcell, and S. U. Emerson. 1998. Genetic and experimental evidence for cross-species infection by swine hepatitis E virus. J. Virol. 72:9714-9721.
25. Meng, X. J., R. H. Purcell, P. G. Halbur, J. R. Lihman, D. M. Webb, T. S. Tsareva, J. S. Haynes, B. J. Thacker, and S. U. Emerson. 1997. A novel virus in swine is closely related to the human hepatitis E virus. Proc. Natl. Acad. Sci. USA 94:9860-9865.
26. Meng, X. J., B. Wiseman, F. Elvinger, D. K. Guenette, T. E. Toth, R. E. Engle, S. U. Emerson, and R. H. Purcell. 2002. Prevalence of antibodies to hepatitis E virus in veterinarians working with swine and in normal blood donors in the United States and other countries. J. Clin. Microbiol. 40:117-122.
27. Panda, S. K., I. H. Ansari, H. Durgapal, S. Agrawal, and S. Jameel. 2000. The in vitro-synthesized RNA from a cDNA clone of hepatitis E virus is infectious. J. Virol. 74:2430-2437.
28. Polo, J. M., and R. E. Johnston. 1990. Attenuating mutations in glycoproteins E1 and E2 of Sindbis virus produce a highly attenuated strain when combined in vitro. J. Virol. 64:4438-4444.
29. Pugachev, K. V., M. S. Galinski, and T. K. Frey. 2000. Infectious cDNA clone of the RA27/3 vaccine strain of rubella virus. Virology 273:189-197.
30. Tei, S., N. Kitajima, K. Takahashi, and S. Mishiro. 2003. Zoonotic transmission of hepatitis E virus from deer to human beings. Lancet 362:371-373.
31. Thomas, D. L., P. O. Yarbough, D. Vlahov, S. A. Tsarev, K. E. Nelson, A. J. Saah, and R. H. Purcell. 1997. Seroreactivity to hepatitis E virus in areas where the disease is not endemic. J. Clin. Microbiol. 35:1244-1247.
32. van Loon, A. A., N. de Haas, I. Zeyda, and E. Mundt. 2002. Alteration of amino acids in VP2 of very virulent infectious bursal disease virus results in tissue culture adaptation and attenuation in chickens. J. Gen. Virol. 83:121-129.
33. Westrop, G. D., K. A. Wareham, D. M. Evans, G. Dunn, P. D. Minor, D. I. Magrath, F. Taffs, S. Marsden, M. A. Skinner, G. C. Schild, and J. W. Almond. 1989. Genetic basis of attenuation of the Sabin type 3 oral polio-virus vaccine. J. Virol. 63:1338-1344.
34. Williams, T. P., C. Kasorndorkbua, P. G. Halbur, G. Haqshenas, D. K. Guenette, T. E. Toth, and X. J. Meng. 2001. Evidence of extrahepatic sites of replication of the hepatitis E virus in a swine model. J. Clin. Microbiol. 39: 3040-3046.
35. Yazaki, Y., H. Mizuo, M. Takahashi, T. Nishizawa, N. Sasaki, Y. Gotanda, and H. Okamoto. 2003. Sporadic acute or fulminant hepatitis E in Hokkaido, Japan, may be food-borne, as suggested by the presence of hepatitis E virus in pig liver as food. J. Gen. Virol. 84:2351-2357.
36. Zafrullah, M., M. H. Ozdener, R. Kumar, S. K. Panda, and S. Jameel. 1999. Mutational analysis of glycosylation, membrane translocation, and cell surface expression of the hepatitis E virus ORF2 protein. J. Virol. 73:4074-4082.