Determination of ploidy and homozygosity of carrot plants obtained from anther cultures

Acta Physiologiae Plantarum

Volumn 33, Issue 2, 2011, Pages 401-407

  Kiszczak, Waldemar ^a   Krzyżanowska, Dorota ^a   Strycharczuk, Krystyna ^c   Kowalska, Urszula ^a   Wolko, Bogdan ^b   Górecka, Krystyna ^a  

^a RESEARCH INSTITUTE OF HORTICULTURE   (Poland)

^b INSTITUTE OF PLANT GENETICS   (Poland)

^c Kutno Sugar Beet Breeding Company   (Poland)

Author keywords

Androgenetic plant; Carrot (Daucus carota L.); Flow cytometry; Histogram; Homozygosity; Isozyme; Ploidy

Indexed keywords

DAUCUS CAROTA;

EID: 79951773485     PISSN: 01375881     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11738-010-0559-x     Document Type: Article

References (27)

1. Andersen SB, Christiansen J, Farestveit B (1990) Carrot (Daucus carota L.): In vitro production of haploids and field trials. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 12. Springer, Berlin, pp 393-402.
2. Bartošová Z, Obert B, Takáč T, Kormuťák A, Preťová A (2005) Using enzyme polymorphism to identify the gametic origin of flax regenerants. Acta Biol Crac Ser Botanica 47(1): 173-178.
3. Cardoso MB, Kaltchuk-Santos E, de Mundstock EC, Bodanese-Zanettini MH (2004) Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling. Braz Arch Biol Technol 47(5): 703-712.
4. Chen CC, Kasha KJ, Marsolais A (1984) Segmentation patterns and mechanisms of genome multiplication in cultured microspores of barley. Can J Genet Cytol 26: 475-483.
5. de Pentado OMI, Garcia P, de la Perez Vega MP (1997) Isozyme markers and genetic variability in three species of Centrosema (Leguminosae). Braz J Genet 20(3): 443-452.
6. Ferrie AMR (2007) Doubled haploid production in nutraceutical species: a review. Euphytica 158: 347-357.
7. Funasaka T, Yanagawa T, Hogan V, Raz A (2005) Regulation of phosphoglucose isomerase/autocrine motility factor expression by hypoxia. FASEB J 19: 1422-1430.
8. Galbraith DW (1984) Flow cytometric analysis of the cell cycle. Cell Cult Som Cell Genet Plants 1: 765-777.
9. Górecka K, Krzyżanowska D, Górecki R (2005a) The influence of several factors on the efficiency of androgenesis in carrot. J Appl Genet 46(3): 265-269.
10. Górecka K, Krzyżanowska D, Kiszczak W, Górecki R (2005b) Embryo induction in anther culture of Daucus carota L. Veget Crops Res Bull 63: 25-32.
11. Górecka K, Krzyżanowska D, Kiszczak W, Kowalska U (2009a) Carrot doubled haploids. In: Touraev A, Forster BP, Mohan Jain S (eds) Advances in haploid production in higher plants. Springer, New York, pp 231-239.
12. Górecka K, Krzyżanowska D, Kiszczak W, Kowalska U (2009b) Plant regeneration from carrot (Daucus carota L.) anther culture derived embryos. Physiol Plant 31: 1139-1145.
13. Gottlieb LD (1973) Enzyme differentiation and phylogeny in Clarkia franciscana, C. rubicunda and C. amoena. Evolution 27: 205-214.
14. Höfer M, Touraev A, Heberle-Bors E (1999) Induction of embryogenesis from isolated apple microspores. Plant Cell Rep 18: 1012-1017.
15. Hu KL, Matsubara S, Murakami K (1993) Haploid plant production by anther culture in carrot (Daucus carota L.). J Japan Soc Hort Sci 62(3): 561-565.
16. Matsubara S, Dohya N, Murakami K, Nishio T, Dore C (1995) Callus formation and regeneration of adventitious embryos from carrot, fennel and mitsuba microspores by anther and isolated microspore cultures. Acta Hort 392: 129-137.
17. Selander RK, Smith MH, Yang SY, Johnson WE, Gentry JB (1971) Biochemical polymorphism and systematics in the genus Peromyscus. I. Variation in the old-field mouse (Peromyscus polionotus). Univ Tex Publ 7103: 49-90.
18. Silvente S, Camas A, Lara M (2003) Molecular cloning of the cDNA encoding aspartate aminotransferase from bean root nodules and determination of its role in nodule nitrogen metabolism. J Exp Bot 54(387): 1545-1551.
19. Smykalová I, Šmirous P Jr, Kubošiová A, Gasmanová N, Griga M (2005) Dihaploid production via anther culture in Czech breeding lines of caraway (Carum carvi L.). Abstracts XII International Conference on Plant Embryology, September 5-7 2005 Cracow, Poland. Acta Biol Crac Ser Botanica 47(1): 52.
20. Song W (2001) Identification of different maize (Zea mays L.) genotypes using isozyme and RAPD marker analysis. Master dissertation, Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland.
21. Sunderland N, Collins GB, Dunwell JM (1974) The role of nuclear fusion in pollen embryogenesis of Datura innoxia Mill. Planta 117: 227-241.
22. Szklarczyk M (1996) Isoenzymatic markers. In: Michalik B (ed) The use of biotechnological methods in plant breeding. Drukpol, Kraków, pp 90-98 (in polish).
23. Testillano P, Georgiev S, Mogensen HL, Coronado MJ, Dumas C, Risueno MC, Matthys-Rochon E (2004) Spontaneous chromosome doubling results from nuclear fusion during in vitro maize induced microspore embryogenesis. Chromosoma 112: 342-349.
24. Tyukavin GB, Shmykova NA, Mankhova MA (1999) Cytological study of embryogenesis in cultured carrot anthers. Russ J Plant Physiol 46(6): 876-884.
25. Weeden FN, Gottlieb LD (1980) Isolation of cytoplasmic enzymes from pollen. Plant Phys 66: 400-403.
26. Westphal L, Wricke G (1989) Genetic analysis of DIA, GOT and PGI isozyme loci in Daucus carota L. ssp. sativus. Plant Breed 102: 51-57.
27. Zeidler M (2000) Electrophoretic analysis of plant isozymes. Act Univ Palacki Olomun Fac Rer Nat Biol 38: 7-16.