Molecular cloning of the cDNA encoding aspartate aminotransferase from bean root nodules and determination of its role in nodule nitrogen metabolism

Journal of Experimental Botany

Volumn 54, Issue 387, 2003, Pages 1545-1551

  Silvente, Sonia ^a   Camas, Alberto ^a   Lara, Miguel ^a  

^a INSTITUTO DE CIENCIAS FÍSICAS   (Mexico)

Author keywords

Aspartate aminotransferase; Bean; cDNA; Molecular cloning; Nodules; Phaseolus vulgaris

Indexed keywords

UREIDES;

AMINO ACIDS; CLONING; DNA; GENES; METABOLISM; NITROGEN COMPOUNDS; PLANTS (BOTANY); RNA; SYNTHESIS (CHEMICAL);

CROPS;

ACETYLENE; AMINO ACID SEQUENCE; ASPARTATE AMINOTRANSFERASES; BASE SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; ISOENZYMES; MOLECULAR SEQUENCE DATA; NITROGEN; NITROGEN COMPOUNDS; PHASEOLUS; PLANT PROTEINS; PLANT ROOTS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID;

AMIDES; CLONES; FARM CROPS; GENES; METABOLISM; NUCLEIC ACIDS; SYNTHESIS;

EMBRYOPHYTA; FABACEAE; PHASEOLUS (ANGIOSPERM); PHASEOLUS VULGARIS;

EID: 0038388949     PISSN: 00220957     EISSN: None     Source Type: Journal    
DOI: 10.1093/jxb/erg161     Document Type: Article

References (37)

1. Appels MA, Haaker H. 1991. Glutamate oxalacetate transaminase in pea root nodules. Plant Physiology 95, 740-747.
2. Boland MJ, Hanks JF, Reynolds PHS, Blevins DG, Tolbert NE, Schubert KR, 1982. Subcellular organization of ureide biogenesis from glycolytic intermediates and ammonium in nitrogen-fixing soybean nodules. Planta 155, 45-51.
3. Bryan JK. 1980. Synthesis of the aspartate family and branched-chain amino acids. In: Miflin BJ, ed. The biochemistry of plants: a comprehensive treatise, vol. 5. New York: Academic Press, 403-452.
4. Cooper AJ, Meister A. 1985. Metabolic significance of transamination. In: Christen P, Metzler DE, eds. Transaminases. New York: John Wiley & Sons, 533-563.
5. Dart PJ, Day JM, Harris D. 1972. Assay of nitrogenase activity by acetylene reduction. In: Use of isotopes for study of fertilizer utilization by legume crops. FAO/IAEA Technical Report Series 149, 85-97.
6. de Silva M, Purcell LC, King CA. 1996. Soybean petiole ureide response to water deficits and decreased transpiration. Crop Science 36, 611-616.
7. Farnham MW, Miller SS, Griffith SM, Vance CP. 1990. Aspartate aminotransferase in alfalfa root nodules. II. Immunological distinction between two forms of the enzyme. Plant Physiology 93, 603-610.
8. Gantt JS, Larson RJ, Farnham MW, Pathirana SM, Miller SS, Vance CP. 1992. Aspartate aminotransferase in effective and ineffective alfalfa nodules. Plant Physiology 98, 868-878.
9. Givan CV. 1980. Aminotransferases in higher plants. In: Stumpf PK, Conn EE, eds. The biochemistry of plants, Vol. 5. New York: Academic Press, 329-357.
10. Gregerson RG, Miller SS, Petrowski M, Gantt JS, Vance CP. 1994. Genomic structure, expression and evolution of the alfalfa aspartate aminotransferase genes. Plant Molecular Biology 25, 387-399.
11. Gregerson RG, Petrowski M, Larson RL, Gantt JS, Vance CP. 1993. Molecular analysis of allelic polymorphism at the AAT2 locus of alfalfa. Molecular and General Genetics 241, 124-128.
12. Griffith SM, Vance CP. 1989. Aspartate aminotransferase in alfalfa root nodules. Purification and partial characterization. Plant Physiology 90, 1622-1629.
13. Jensen RA, Calhoun DH. 1981. Intracellular roles of microbial aminotransferases: overlap enzymes across different biochemical pathways. Critical Review in Microbiology 8, 229-266.
14. Joshi CP. 1987. Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis. Nucleic Acids Research 15, 9624-9640.
15. Keegstra K, Olsen LJ, Theg SM. 1989. Chloroplastic precursors and their transport across the envelope membranes. Annual Review of Plant Physiology 40, 471-501.
16. 2 diffusion in legume nodules. Plant Physiology 113, 259-267.
17. Purcell LC, Serraj R, de Silva M, Sinclair TR, Bona S. 1998. Ureide concentration of field-grown soybean in response to drought and the relationship to nitrogen fixation. Journal of Plant Nutrition 21, 949-966.
18. Reynolds PHS, Farnden KJF. 1979. The involvement of aspartate aminotransferases in ammonium assimilation in lupin nodules. Phytochemistry 18, 1625-1630.
19. Reynolds PHS, Smith LA, Dickson JMJJ, Jones WT, Jones SD, Rodber KA, Came A, Liddane CP. 1992. Molecular cloning of a cDNA encoding aspartate aminotransferase-P2 from lupin root nodules. Plant Molecular Biology 19, 465-472.
20. Ryan E, Bodley F, Fottrell PF. 1972. Purification and characterisation of aspartate aminotransferases from soybean root nodules and Rhizobium japonicum. Phytochemistry 11, 957-963.
21. Ryan E, Fottrell PF. 1974. Subcellular localization of enzymes involved in the assimilation of ammonia by soybean root nodules. Plant Molecular Biology 19, 2647-2652.
22. Schultz CJ, Coruzzi GM. 1995. The aspartate aminotransferase gene family of Arabidopsis encodes isozymes localized to three distinct subcellular compartments. The Plant Journal 7, 61-75.
23. Schultz CJ, Hsu M, Miesak B, Coruzzi GM. 1998. Arabidopsis mutants define an in vivo role for isoenzymes of aspartate aminotransferase in plant nitrogen assimilation. Genetics 149, 491-499.
24. Serraj R, Vadez V, Denison RF, Sinclair TR. 1999. Involvement of ureides in nitrogen fixation inhibition in soybean. Plant Physiology 199, 289-296.
25. Silvente S, Blanco L, Camas A, Ortega JL, Ramírez M, Lara-Flores M. 2002. Rhizobium etli mutant modulates carbon and nitrogen metabolism in Phaseolus vulgaris nodules. Molecular Plant-Microbe Interactions 15, 728-733.
26. Sinclair TR, Serraj R. 1995. Dinitrogen fixation sensitivity to drought among grain legume species. Nature 378, 344.
27. 4 plant: comparison of enzymatic properties, primary structures, and expression patterns. Archives in Biochemistry and Biophysics 318, 295-306.
28. 4 plant. European Journal of Biochemistry 204, 611-620.
29. Turano FJ, Weisemann JM, Mathews BF. 1992. Identification and expression of a cDNA clone encoding aspartate aminotransferase in carrot. Plant Physiology 100, 374-381.
30. Udvardi MK, Kahn ML. 1991. Isolation and analysis of a cDNA clone that encodes an alfalfa (Medicago sativa) aspartate aminotransferase. Molecular and General Genetics 231, 97-105.
31. Vance CP, Gregerson RG, Robinson DL, Miller SS, Gant JS. 1994. Primary assimilation of nitrogen in alfalfa nodules: molecular features of the enzymes involved. Plant Science 101, 51-64.
32. Vogels GD, Van Der Drifft C. 1970. Differential analyses of glyoxylate derivatives. Analytical Biochemistry 33, 143-157.
33. Wadsworth GJ, Gebhardt JS, Matthews BF. 1994. Characterization of a soybean cDNA clone encoding the mitochondrial isozyme of aspartate aminotransferase, AAT4. Plant Molecular Biology 27, 1085-1095.
34. Wadsworth GJ, Marmaras SM, Matthews BF. 1993. Isolation and characterization of a soybean cDNA clone encoding the plastid form of aspartate aminotransferase. Plant Molecular Biology 21, 993-1009.
35. Wallsgrove RM, Keys AJ, Lea PJ, Miflin BJ. 1983. Photosynthesis, photorespiration and nitrogen metabolism. Plant, Cell and Environment 6, 301-309.
36. Wilkie CS, Roper JM, Smith AG, Warren MJ. 1995. Isolation and characterization and expression of a cDNA clone encoding plastid aspartate aminotransferase from Arabidopsis thaliana. Plant Molecular Biology 27, 1227-1233.
37. 1 from Lupinus angustifolius root tips. Plant Physiology 104, 417-423.