Pulmonary tuberculosis in the West Bank, Palestinian Authority: Molecular diagnostic approach

Tropical Medicine and International Health

Volumn 16, Issue 3, 2011, Pages 360-367

  Ereqat, Suheir ^a,b   Bar Gal, Gila K ^b   Nasereddin, Abedelmajeed ^a   Said, Sawsan ^c   Greenblatt, Charles L ^b   Azmi, Kifaya ^a   Qaddomi, Sharif E ^a   Spigelman, Mark ^b,d   Ramlawi, Asad ^c   Abdeen, Ziad ^a  

^a AL QUDS UNIVERSITY   (Palestine)

^b HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^c Palestinian Ministry of Health   (Palestine)

^d UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

IS6110; Palestinian territories; Pulmonary tuberculosis; Rifampin resistance; rpoBgene

Indexed keywords

RIFAMPICIN; RNA POLYMERASE BETA SUBUNIT;

BIOASSAY; DISEASE CONTROL; DISEASE TREATMENT; DRUG RESISTANCE; MOLECULAR ANALYSIS; MUTATION; POLYMERASE CHAIN REACTION; TUBERCULOSIS;

ADULT; ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATE; COMPARATIVE EFFECTIVENESS; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; DNA SEQUENCE; DRUG RESISTANT TUBERCULOSIS; EARLY DIAGNOSIS; FEMALE; GENE INSERTION SEQUENCE; GENE MUTATION; HUMAN; LUNG TUBERCULOSIS; MAJOR CLINICAL STUDY; MALE; MOLECULAR DIAGNOSIS; MYCOBACTERIUM TUBERCULOSIS; NUCLEOTIDE SEQUENCE; PALESTINE; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; SPUTUM SMEAR;

ADULT; AMINO ACID SEQUENCE; ANTIBIOTICS, ANTITUBERCULAR; BACTERIAL PROTEINS; FEASIBILITY STUDIES; FEMALE; HUMANS; MALE; MIDDLE AGED; MIDDLE EAST; MOLECULAR SEQUENCE DATA; MUTATION; MYCOBACTERIUM TUBERCULOSIS; POLYMERASE CHAIN REACTION; RIFAMPIN; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; SPUTUM; TUBERCULOSIS, PULMONARY;

PALESTINE;

MYCOBACTERIUM TUBERCULOSIS;

EID: 79951728625     PISSN: 13602276     EISSN: 13653156     Source Type: Journal    
DOI: 10.1111/j.1365-3156.2010.02697.x     Document Type: Article

References (32)

1. Ani A, Okpe S, Akambi M et al. (2009) Comparison of a DNA based PCR method with conventional methods for the detection of M. tuberculosis in Jos, Nigeria. Journal of Infection in Developing Countries 3, 470-475.
2. Beige J, Lokies J, Schaberg T et al. (1995) Clinical evaluation of a Mycobacterium tuberculosis PCR assay. Journal of Clinical Microbiology 33, 90-95.
3. Cavusoglu C, Hilmioglu S, Guneri S & Bilgic A (2002) Characterization of rpoB mutations in rifampin-resistant clinical isolates of Mycobacterium tuberculosis from Turkey by DNA sequencing and line probe assay. Journal of Clinical Microbiology 40, 4435-4438.
4. Clarridge JE, Shawar RM, Shinnick TM & Plikaytis BB (1993) Large-scale use of polymerase chain reaction for detection of Mycobacterium tuberculosis in a routine mycobacteriology laboratory. Journal of Clinical Microbiology 31, 2049-2056.
5. Daley P, Latha A, Mathai D et al. (2009) A pilot study of short-duration sputum pretreatment procedures for optimizing smear microscopy for tuberculosis. PLoS ONE 4, e5626.
6. Drobniewski FA & Wilson SM (1998) The rapid diagnosis of isoniazid and rifampicin resistance in Mycobacterium tuberculosis--a molecular story. Journal of Medical Microbiology 47, 189-196.
7. Eisenach KD, Cave MD, Bates JH & Crawford JT (1990) Polymerase chain reaction amplification of a repetitive DNA sequence specific for Mycobacterium tuberculosis. Journal of Infectious Diseases 161, 977-981.
8. Eisenach KD, Sifford MD, Cave MD, Bates JH & Crawford JT (1991) Detection of Mycobacterium tuberculosis in sputum samples using a polymerase chain reaction. The American Review of Respiratory Disease 144, 1160-1163.
9. Evans JT, Parveen A, Smith GE et al. (2009) Application of denaturing HPLC to rapidly identify rifampicin-resistant Mycobacterium tuberculosis in low- and high-prevalence areas. Journal of Antimicrobial Chemotherapy 63, 295-301.
10. Garcia-Quintanilla A, Garcia L, Tudo G, Navarro M, Gonzalez J & Jimenez de Anta MT (2000) Single-tube balanced heminested PCR for detecting Mycobacterium tuberculosis in smear-negative samples. Journal of Clinical Microbiology 38, 1166-1169.
11. Gopinath K & Singh S (2009) Multiplex PCR assay for simultaneous detection and differentiation of Mycobacterium tuberculosis, Mycobacterium avium complexes and other Mycobacterial species directly from clinical specimens. Journal of Applied Microbiology 107, 425-435.
12. Haldar S, Chakravorty S, Bhalla M, De Majumdar S & Tyagi JS (2007) Simplified detection of Mycobacterium tuberculosis in sputum using smear microscopy and PCR with molecular beacons. Journal of Medical Microbiology 56, 1356-1362.
13. Hashimoto T, Suzuki K, Amitani R & Kuze F (1995) Rapid detection of Mycobacterium tuberculosis in sputa by the amplification of IS6110. Internal Medicine 34, 605-610.
14. Heep M, Brandstatter B, Rieger U et al. (2001) Frequency of rpoB mutations inside and outside the cluster I region in rifampin-resistant clinical Mycobacterium tuberculosis isolates. Journal of Clinical Microbiology 39, 107-110.
15. Hellyer TJ, DesJardin LE, Assaf MK, Bates JH, Cave MD & Eisenach KD (1996) Specificity of IS6110-based amplification assays for Mycobacterium tuberculosis complex. Journal of Clinical Microbiology 34, 2843-2846.
16. Hoek KG, Gey van Pittius NC, Moolman-Smook H et al. (2008) Fluorometric assay for testing rifampin susceptibility of Mycobacterium tuberculosis complex. Journal of Clinical Microbiology 46, 1369-1373.
17. Lima SS, Clemente WT, Palaci M, Rosa RV, Antunes CM & Serufo JC (2008) Conventional and molecular techniques in the diagnosis of pulmonary tuberculosis: a comparative study. Jornal Brasileiro De Pneumologia 34, 1056-1062.
18. Mani C, Selvakumar N, Narayanan S & Narayanan PR (2001) Mutations in the rpoB gene of multidrug-resistant Mycobacterium tuberculosis clinical isolates from India. Journal of Clinical Microbiology 39, 2987-2990.
19. Palomino JC (2005) Nonconventional and new methods in the diagnosis of tuberculosis: feasibility and applicability in the field. European Respiratory Journal 26, 339-350.
20. Patnaik M, Liegmann K & Peter JB (2001) Rapid detection of smear-negative Mycobacterium tuberculosis by PCR and sequencing for rifampin resistance with DNA extracted directly from slides. Journal of Clinical Microbiology 39, 51-52.
21. Pietzka AT, Indra A, Stoger A et al. (2009) Rapid identification of multidrug-resistant Mycobacterium tuberculosis isolates by rpoB gene scanning using high-resolution melting curve PCR analysis. Journal of Antimicrobial Chemotherapy 63, 1121-1127.
22. Rajalahti I, Vuorinen P, Nieminen MM & Miettinen A (1998) Detection of Mycobacterium tuberculosis complex in sputum specimens by the automated Roche Cobas Amplicor Mycobacterium Tuberculosis Test. Journal of Clinical Microbiology 36, 975-978.
23. Salian NV, Rish JA, Eisenach KD, Cave MD & Bates JH (1998) Polymerase chain reaction to detect Mycobacterium tuberculosis in histologic specimens. American Journal of Respiratory and Critical Care Medicine 158, 1150-1155.
24. Soudani A, Hadjfredj S, Zribi M et al. (2007) Characterization of Tunisian Mycobacterium tuberculosis rifampin-resistant clinical isolates. Journal of Clinical Microbiology 45, 3095-3097.
25. Srisuwanvilai LO, Monkongdee P, Podewils LJ et al. (2008) Performance of the BACTEC MGIT 960 compared with solid media for detection of Mycobacterium in Bangkok, Thailand. Diagnostic Microbiology and Infectious Disease 61, 402-407.
26. Sun JR, Lee SY, Perng CL & Lu JJ (2009) Detecting Mycobacterium tuberculosis in Bactec MGIT 960 cultures by inhouse IS6110-based PCR assay in routine clinical practice. Journal of the Formosan Medical Association 108, 119-125.
27. Suresh N, Singh UB, Gupta C, Arora J, Rana T & Samantaray JC (2007a) Rapid detection of rifampin-resistant Mycobacterium tuberculosis directly from stained sputum smears using single-tube nested polymerase chain reaction deoxyribonucleic acid sequencing. Diagnostic Microbiology and Infectious Disease 58, 217-222.
28. Suresh N, Arora J, Pant H, Rana T & Singh UB (2007b) Spoligotyping of Mycobacterium tuberculosis DNA from Archival Ziehl-Neelsen-stained sputum smears. Journal of Microbiol Methods 68, 291-295.
29. Van Der Zanden AG, Te Koppele-Vije EM, Vijaya Bhanu N, Van Soolingen D & Schouls LM (2003) Use of DNA extracts from Ziehl-Neelsen-stained slides for molecular detection of rifampin resistance and spoligotyping of Mycobacterium tuberculosis. Journal of Clinical Microbiology 41, 1101-1108.
30. WHO (2009) Title of page? Available at
31. Williams DL, Waguespack C, Eisenach K et al. (1994) Characterization of rifampin-resistance in pathogenic mycobacteria. Antimicrobial Agents and Chemotherapy 38, 2380-2386.
32. Zink A, Haas CJ, Reischl U, Szeimies U & Nerlich AG (2001) Molecular analysis of skeletal tuberculosis in an ancient Egyptian population. Journal of Medical Microbiology 50, 355-366.