Single-tube balanced heminested PCR for detecting Mycobacterium tuberculosis in smear-negative samples

Journal of Clinical Microbiology

Volumn 38, Issue 3, 2000, Pages 1166-1169

  García Quintanilla, Albert ^a   Garcia, Lourdes ^c   Tudó, Griselda ^a   Navarro, Maria ^a   González, Julià ^b   Jiménez De Anta, Maria T ^b  

^a UNIVERSITY OF BARCELONA   (Spain)

^b HOSPITAL CLÍNIC   (Spain)

^c UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM CULTURE; CONTROLLED STUDY; HUMAN; MAJOR CLINICAL STUDY; MYCOBACTERIUM TUBERCULOSIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SPUTUM; TUBERCULOSIS;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS; UNCULTURED ACTINOMYCETE;

EID: 0343986451     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.38.3.1166-1169.2000     Document Type: Article

References (24)

1. American Thoracic Society Workshop. 1997. Rapid diagnostic tests for tuberculosis. What is the appropriate use? Am. J. Respir. Crit. Care Med. 155:1804-1814.
2. Behr, M. A., S. A. Warren, H. Salamon, P. C. Hopewell, A. Ponce de Leon, C. L. Daley, P. M. Small. 1999. Transmission of Mycobacterium tuberculosis from patients smear-negative for acid-fast bacilli. Lancet 353:444-449.
3. Bennedsen, J., V. O. Thomson, G. E. Pfyffer, G. Funke, K. Feldmann, A. Beneke, et al. 1996. Utility of PCR in diagnosing pulmonary tuberculosis. J. Clin. Microbiol. 34:1407-1411.
4. Brugiere, O., M. Vokurka, D. Lecossier, G. Mangiapan, A. Amrane, B. Milleron, C. Mayaud, J. Cadranel, and A. J. Hance. 1997. Diagnosis of smear-negative pulmonary tuberculosis using sequence capture polymerase chain reaction. Am. J. Respir. Crit. Care Med. 155:1478-1481.
5. Chan, C. M., K. Y. Yuen, K. S. Chan, W. C. Yam, K. H. M. Yim, W. F. Ng, and M. H. Ng. 1996. Single-tube nested PCR in the diagnosis of tuberculosis. J. Clin. Pathol. 49:290-294.
6. Chou, Q., M. Russell, D. E. Birch, J. Raymond, and W. Block. 1992. Prevention of pre-PCR mis-priming and primer dimerization improves low-copy number amplifications. Nucleic Acids Res. 20:1717-1723.
7. Clarridge, J. E., III, R. M. Shawar, T. M. Shinnick, and B. B. Plikaytis. 1993. Large-scale use of polymerase chain reaction for detection of Mycobacleriutn tuberculosis in a routine mycobacteriology laboratory. J Clin. Microbiol. 31:2049-2056.
8. Colt, S. T., R. Brosch, J. Parkhill, T. Garnier, C. Churcher, D. Harris, S. V. Gordon, K. Eiglmeier, S. Gas, C. E. Barry III, F. Tekaia, K. Badcock, D. Basham, D. Brown, et al. 1998. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393:537-544.
9. Doern, G. V. 1996. Diagnostic mycobacteriology: where are we today? J. Clin. Microbiol. 34:1873-1876.
10. Kennedy, N., S. H. Gillespie, A. O. S. Saruni, G. Kisyombe, R. McNerney, F. I. Ngowi, and S. Wilson. 1994. Polymerase chain reaction for assessing treatment response in patients with pulmonary tuberculosis. J. Infect. Dis. 170:713-716.
11. Kocagoz, T., E. Yilmaz, S. Ozkara, S. Kocagoz, M. Hayran, M. Sachedeva, and H. F. Chambers. 1993. Detection of Mycobacterium tuberculosis in sputum samples by polymerase chain reaction using a simplified procedure. J. Clin. Microbiol. 31:1435-1438.
12. Kubica, G. P. W., E. Dye, M. L. Cohn, and G. Middlebrook. 1963. Sputum digestion and decontamination with N-acetyl-L-cysteine-sodium hydroxide for culture of mycobacteria. Am. Rev. Respir. Dis. 87:775-779.
13. Lebrun, L., D. Mathieu, C. Saulnier, and P. Nordmann. 1997. Limits of commercial molecular tests for diagnosis of pulmonary tuberculosis. Eur. Respir. J. 10:1874-1876.
14. Liu, P. Y.-F., Z.-Y. Shi, Y.-J. Lau, and B.-S. Hu. 1994. Rapid diagnosis of tuberculous meningitis by a simplified nested amplification protocol. Neurology 44:1161-1164.
15. Longo, M. C., M. S. Berninger, and J. L. Hartley. 1990. DNA glycosylase to control carry-over contamination in polymerase chain reactions. Gene 93: 125-128.
16. Nolte, F. S., B. Metchock, J. E. McGowan, Jr., A. Edwards, O. Kwumabua, C. Thurmond, et al. 1993. Direct detection of Mycobacterium tuberculosis in sputum by polymerase chain reaction and DNA hybridization. J. Clin. Microbiol. 31:1777-1782.
17. Noordhoek, G. T., A. H. J. Kolk, G. Bjune, D. Catty, J. W. Dale, P. E. M. Fine, P. Godfrey-Faussett, S.-N. Cho, T. Shinnick, S. B. Svenson, S. Wilson, and J. D. A. van Embden. 1994. Sensitivity and specificity of PCR for detection of Mycobacterium tuberculosis: a blind comparison study among seven laboratories. J. Clin. Microbiol. 32:277-284.
18. Pierre, C., D. Lecossier, Y. Boussougant, D. Bocart, V. Joly, P. Yeni, et al. 1991. Use of a reamplification protocol improves sensitivity of detection of Mycobacterium tuberculosis in clinical samples by amplification of DNA. J. Clin. Microbiol. 29:712-717.
19. Roth, A., T. Schaberg, and H. Mauch. 1997. Molecular diagnosis of tuberculosis: current clinical validity and future perspectives. Eur. Respir. J. 10: 1877-1891.
20. Shawar, R. M., F. A. K. El Zaatari, A. Nataraj, and J. E. Clarridge. 1993. Detection of Mycobacterium tuberculosis in clinical samples by two-step polymerase chain reaction and nonisotopic hybridization methods. J. Clin. Microbiol. 31:61-65.
21. Thierry, D., M. D. Cave, K. D. Eisenach, J. T. Crawford, J. H. Bates, B. Gicquel, et al. 1990. IS6110, an IS-like element of Mycobaclerium tuberculosis complex. Nucleic Acids Res. 18:188.
22. Wilson, S. M., R. McNerney, P. M. Nye, P. D. Godfrey-Faussett, N. G. Stoker, and A. Voller. 1993. Progress toward a simplified polymerase chain reaction and its application to diagnosis of tuberculosis. J. Clin. Microbiol. 31:776-782.
23. World Health Organization. 1998. WHO report on the tuberculosis epidemic 1997. WHO, Geneva, Switzerland.
24. Yuen, K., W. C. Yam, L. P. Wong, and W. H. Seto. 1997. Comparison of two automated DNA amplification systems with a manual one-tube nested PCR assay for diagnosis of pulmonary tuberculosis. J. Clin. Microbiol. 35:1385-1389.