The effect of the feed-to-buffer ratio on bacterial diversity and ruminal fermentation in single-flow continuous-culture fermenters

Journal of Dairy Science

Volumn 94, Issue 3, 2011, Pages 1374-1384

  Cantalapiedra Hijar, G ^a   Yanez Ruiz D R ^a   Newbold, C J ^b   Molina Alcaide, E ^a  

^a CSIC   (Spain)

^b ABERYSTWYTH UNIVERSITY   (United Kingdom)

Author keywords

Bacterial diversity; Continuous culture fermenter; Feed buffer; Ruminal fermentation

Indexed keywords

BUFFER;

ANIMAL; ANIMAL DISEASE; ANIMAL FOOD; ARTICLE; BIOREACTOR; CHEMISTRY; DIET; EVALUATION; FERMENTATION; GOAT; METABOLISM; MICROBIOLOGY; PH; PHYSIOLOGY; RUMINANT STOMACH;

ANIMAL FEED; ANIMAL NUTRITIONAL PHYSIOLOGICAL PHENOMENA; ANIMALS; BIOREACTORS; BUFFERS; DIET; FERMENTATION; GOATS; HYDROGEN-ION CONCENTRATION; RUMEN;

BACTERIA (MICROORGANISMS); MEDICAGO SATIVA;

EID: 79951718653     PISSN: 00220302     EISSN: 15253198     Source Type: Journal    
DOI: 10.3168/jds.2010-3260     Document Type: Article

References (50)

1. Association of Official Analytical Chemists. Official methods of analysis of the AOAC 2005, AOAC, AOAC, Arlington, VA. 18th Ed.
2. Bach A., Calsamiglia S., Stern M.D. Nitrogen metabolism in the rumen. J. Dairy Sci. 2005, 88:E9-E21.
3. Balcells J., Guada J.A., Peiró J.M., Parker D.S. Simultaneous determination of allantoin and oxypurines in biological fluids by high-performance liquid chromatography. J. Chromatogr. 1992, 575:153-157.
4. Calsamiglia S., Cardozo P.W., Ferret A., Bach A. Changes in rumen microbial fermentation are due to a combined effect of type of diet and pH. J. Anim. Sci. 2008, 86:702-711.
5. Calsamiglia S., Ferret A., Devant M. Effects of pH and pH fluctuations on microbial fermentation and nutrient flow from a dual-flow continuous culture system. J. Dairy Sci. 2002, 85:574-579.
6. Cantalapiedra-Hijar G., Yáñez-Ruiz D.R., Martín-García A.I., Molina-Alcaide E. Effects of forage:concentrate ratio and forage type on apparent digestibility, ruminal fermentation, and microbial growth in goats. J. Anim. Sci. 2009, 87:622-631.
7. Carro M.D., Ranilla M.J., Martín-García A.I., Molina-Alcaide E. Comparison of microbial fermentation of high- and low-forage diets in Rusitec, single-flow continuous-culture fermenters and sheep rumen. Animal 2009, 3:527-534.
8. Castillejos L., Calsamiglia S., Ferret A., Losa R. Effects of a specific blend of essential oil compounds and the type of diet on rumen microbial fermentation and nutrient flow from a continuous culture system. Anim. Feed Sci. Technol. 2005, 119:29-41.
9. Cerrato-Sánchez M., Calsamiglia S., Ferret A. Effect of the magnitude of the decrease of rumen pH on rumen fermentation in a dual-flow continuous culture system. J. Anim. Sci. 2008, 86:378-383.
10. Church D.C. Digestive Physiology and Nutrition of Ruminants 1976, I. Digestive Physiology. D. C. Church, Corvallis, OR.
11. Colombatto D., Hervás G., Yang W.Z., Beauchemin K.A. Effects of enzyme supplementation of a total mixed ration on microbial fermentation in continuous culture, maintained at high and low pH. J. Anim. Sci. 2003, 81:2617-2627.
12. Crawford R.J., Hoover W.H., Knowlton P.H. Effects of solids and liquid flows on fermentation in continuous cultures. I. Dry matter and fiber digestion, VFA production and protozoa numbers. J. Anim. Sci. 1980, 51:975-984.
13. Dahlberg E.M., Stern M.D., Ehle F.R. Effects of forage source on ruminal microbial nitrogen metabolism and carbohydrate digestion in continuous culture. J. Anim. Sci. 1988, 66:2071-2083.
14. de Veth M.J., Kolver E.S. Diurnal variation in pH reduces digestion and synthesis of microbial protein when pasture is fermented in continuous culture. J. Dairy Sci. 2001, 84:2066-2072.
15. Devant M., Ferret A., Calsamiglia S., Casals R., Gasa J. Effect of nitrogen source in high-concentrate, low-protein beef cattle diets on microbial fermentation studied in vivo and in vitro. J. Anim. Sci. 2001, 79:1944-1953.
16. Griswold K.E., Apgar G.A., Bouton J., Firkins J.L. Effects of urea infusion and ruminal degradable protein concentration on microbial growth, digestibility, and fermentation in continuous culture. J. Anim. Sci. 2003, 81:329-336.
17. Hannah S.M., Stern M.D., Ehle F.R. Evaluation of a dual flow continuous culture system for estimating bacterial fermentation in vivo of mixed diets containing various soya bean products. Anim. Feed Sci. Technol. 1986, 16:51-62.
18. Hongoh Y., Deevong P., Inoue T., Moriya S., Trakulnaleamsai S., Ohkuma M., Vongkaluang C., Noparatnaraporn N., Kudo T. Intra- and interspecific comparisons of bacterial diversity and community structure support coevolution of gut microbiota and termite host. Appl. Environ. Microbiol. 2005, 71:6590-6599.
19. Hoover W.H., Crooker B.A., Sniffen C.J. Effects of differential solid-liquid removal rates on protozoa numbers in continuous cultures of rumen contents. J. Anim. Sci. 1976, 43:528-534.
20. Isac M.D., García M.A., Aguilera J.F., Molina-Alcaide E. A comparative study of nutrient digestibility, kinetics of digestion and passage and rumen fermentation pattern in goats and sheep offered medium quality forages at the maintenance level of feeding. Arch. Tierernahr. 1994, 46:37-50.
21. Littell R.C., Henry P.R., Ammerman C.B. Statistical analysis of repeated measures data using SAS procedures. J. Anim. Sci. 1998, 76:1216-1231.
22. Liu W.T., Marsh T.L., Cheng H., Forney L.J. Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16rRNA. Appl. Environ. Microbiol. 1997, 63:4516-4522.
23. Mansfield H.R., Endres M.I., Stern M.D. Comparison of microbial fermentation in the rumen of dairy cows and dual flow continuous culture. Anim. Feed Sci. Technol. 1995, 55:47-66.
24. Marsh T.L. Terminal restriction fragment length polymorphism (T-RFLP): An emerging method for characterizing diversity among homologous populations of amplification products. Curr. Opin. Microbiol. 1999, 2:323-327.
25. Marsh T.L., Saxman P., Cole J., Tiedje J. Terminal restriction fragment length polymorphism analysis program, a web-based research tool for microbial community analysis. Appl. Environ. Microbiol. 2000, 66:3616-3620.
26. Martín-Orúe S.M., Balcells J., Zakraoui F., Castrillo C. Quantification and chemical composition of mixed bacteria harvested from solid fractions of rumen digesta: Effect of detachment procedure. Anim. Feed Sci. Technol. 1998, 71:269-282.
27. Martínez M.E., Ranilla M.J., Ramos S., Tejido M.L., Carro M.D. Effects of dilution rate and retention time of concentrate on efficiency of microbial growth, methane production, and ruminal fermentation in Rusitec fermenters. J. Dairy Sci. 2009, 92:3930-3938.
28. McDougall E.I. Studies on ruminant saliva. The composition and output of sheep's saliva. Biochem. J. 1948, 43:99-109.
29. Meng Q., Kerley M.S., Ludden P.A., Belyea R.L. Fermentation substrate and dilution rate interact to affect microbial growth and efficiency. J. Anim. Sci. 1999, 77:206-214.
30. Miettinen H., Setälä J. Design and development of a continuous culture system to study rumen fermentation. J. Agric. Sci. Finland. 1989, 61:463-473.
31. Molina-Alcaide E., Pascual M.R., Cantalapiedra-Hijar G., Morales-García E.Y., Martín-García A.I. Effects of concentrate replacement by feed blocks on ruminal fermentation and microbial growth in goats and single-flow continuous-culture fermenters. J. Anim. Sci. 2009, 87:1321-1333.
32. Muetzel S., Lawrence P., Hoffman E.M., Becker K. Evaluation of a stratified continuous rumen incubation system. Anim. Feed Sci. Technol. 2009, 151:32-43.
33. Obispo N.E., Dehority B.A. Feasibility of using total purines as a marker for ruminal bacteria. J. Anim. Sci. 1999, 77:3084-3095.
34. Osborn A.M., Moore E.R.B., Timmis K.N. An evaluation of terminal-restriction fragment length polymorphism (T-RFLP) analysis for the study of microbial community structure and dynamics. Environ. Microbiol. 2000, 2:39-50.
35. 15N and purine bases as markers of microbial nitrogen entering the duodenum. Br. J. Nutr. 1996, 75:699-709.
36. Russell J.B. The importance of pH in the regulation of ruminal acetate to propionate ratio and methane production in vitro. J. Dairy Sci. 1998, 81:3222-3230.
37. Russell J.B., Dombrowski D.B. Effect of pH on the efficiency of growth by pure cultures of rumen bacteria in continuous culture. Appl. Environ. Microbiol. 1980, 39:604-610.
38. Sait L., Galic M., Strugnell R.A., Janssen P.H. Secretory antibodies do not affect the composition of the bacterial microbiota in the terminal ileum of 10-week-old mice. Appl. Environ. Microbiol. 2003, 69:2100-2109.
39. Shannon C.E., Weaver W. The Mathematical Theory of Communication 1963, University of Illinois Press, Urbana.
40. Shriver B.J., Hoover W.H., Sargent J.P., Crawford R.J., Thayne W.V. Fermentation of a high concentrate diet as affected by ruminal pH and digesta flow. J. Dairy Sci. 1986, 69:413-419.
41. Tapia M.O., Stern M.D., Koski R.L., Bach A., Murphy M.J. Effects of patulin on rumen microbial fermentation in continuous culture fermenters. Anim. Feed Sci. Technol. 2002, 97:239-246.
42. Tilley J.M.A., Terry R.A. A two-stage technique for the in vitro digestion of forage crops. J. Br. Grassl. Soc. 1963, 18:104-111.
43. Van Soest P.J., Robertson J.B., Lewis B.A. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 1991, 74:3583-3597.
44. Wales W.J., Kolver E.S., Thorne P.L., Egan A.R. Diurnal variation in ruminal pH on the digestibility of highly digestible perennial ryegrass during continuous culture fermentation. J. Dairy Sci. 2004, 87:1864-1871.
45. Wallace R.J., Cotta M.A. Metabolism of nitrogen-containing compounds. The Rumen Microbial Ecosystem 1989, 217-250. Elsevier Applied Science, New York, NY. P.N. Hobson (Ed.).
46. Weatherburn M.W. Phenol-hypochlorite reaction for determination of ammonia. Anal. Chem. 1967, 39:971-974.
47. Winkler P., Ghadimi D., Schrezenmeir J., Kraehenbuhl J.P. Molecular and cellular basis of microflora-host interactions. J. Nutr. 2007, 137(3 Suppl 2):756S-772S.
48. Yang W.Z., Beauchemin K.A., Vedres D.D. Effects of pH and fibrolytic enzymes on digestibility, bacterial protein synthesis, and fermentation in continuous culture. Anim. Feed Sci. Technol. 2002, 102:137-150.
49. Zhang R., Thiyagarajan V., Qian P.-Y. Evaluation of terminal-restriction fragment length polymorphism analysis in contrasting marine environments. FEMS Microbiol. Ecol. 2008, 65:169-178.
50. Ziemer C.J., Sharp R., Stern M.D., Cotta M.A., Whitehead T.R., Stahl D.A. Comparison of microbial populations in model and natural rumens using 16S ribosomal RNA-targeted probes. Environ. Microbiol. 2000, 2:632-643.