Production and evaluation of transgenic sweet orange (Citrus sinensis Osbeck) containing bivalent antibacterial peptide genes (Shiva A and Cecropin B) via a novel Agrobacterium-mediated transformation of mature axillary buds

Scientia Horticulturae

Volumn 128, Issue 2, 2011, Pages 99-107

  He, Yongrui ^a,b   Chen, Shanchun ^a,b   Peng, Aihong ^a,b   Zou, Xiuping ^a,b   Xu, Lanzheng ^a,b   Lei, Tiangang ^a,b   Liu, Xiaofeng ^a,b   Yao, Lixiao ^a,b  

^a INSTITUTE OF PLANT PROTECTION   (China)

^b National Engineering Research Center for Citrus Technology   (China)

Author keywords

Citrus; Citrus canker; Disease resistance; Mature axillary bud; Transformation

Indexed keywords

ACIDITY; AGRICULTURAL PRODUCTION; ANTIMICROBIAL ACTIVITY; BACTERIUM; DEVELOPMENTAL BIOLOGY; DISEASE RESISTANCE; FRUIT; GENE EXPRESSION; GENOME; INOCULATION; PEPTIDE; POLYMERASE CHAIN REACTION; SUGAR; TRANSFORMATION; TRANSGENIC PLANT;

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; CITRUS; CITRUS SINENSIS; PONCIRUS TRIFOLIATA; RHIZOBIUM; XANTHOMONAS AXONOPODIS; XANTHOMONAS AXONOPODIS PV. CITRI;

EID: 79951677872     PISSN: 03044238     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.scienta.2011.01.002     Document Type: Article

References (46)

1. Ali S., Mirza B. Micropropagation of rough lemon (Citrus jambhiri Lush.): effect of explant type and hormone concentration. Acta Bot. Croat. 2006, 65:137-146.
2. An G., Watson B.D., Stachel S., Gordon M.P., Nester E.W. New cloning vehicles for transformation of higher plants. EMBO J. 1985, 4:277-284.
3. Andreu D., Merrifield R.B., Steiner H., Boman H.G. Solid-phase synthesis of Cecropin A and related peptides. Proc. Natl. Acad. Sci. U.S.A. 1983, 80:6475-6479.
4. Barbosa-Mendes J.M., Filho F.A.A.M., Filho A.B., Harakava R., Beer S.V., Mendes B.M.J. Genetic transformation of Citrus sinensis cv. Hamlin with hrpN gene from Erwinia amylovora and evaluation of the transgenic lines for resistance to citrus canker. Sci. Hortic. 2009, 122:109-115.
5. Ballester A., Cervera M., Peña L. Efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination. Plant Cell Rep. 2007, 26:39-45.
6. Boman H.G., Boman A., Pigon A. Immune and injury response in Cecropia pupaerna: isolation and comparison of synthesis in vivo and in vitro. Insect Biochem. 1981, 11:33-42.
7. Boman H.G., Faye I., Gudmundsson G.H., Lee J.Y., Lidholm D.A. Cell-free immunity in Cecropia. A model system for antibacterial proteins. Eur. J. Biochem. 1991, 201:23-31.
8. Boman H.G. Antibacterial peptides: basic facts and emerging concepts. J. Intern. Med. 2003, 254:197-215.
9. Bond J.E., Roose M.L. Agrobacterium-mediated transformation of the commercially important citrus cultivar Washington navel orange. Plant Cell Rep. 1998, 18:229-234.
10. Boscariol R.L., Monteiro M., Takahashi E.K. Attacin A gene from Tricloplusia ni reduces susceptibility to Xanthomonas axonopodis pv. citri in transgenic Citrus sinensis cv. Hamlin. J. Am. Soc. Hortic. Sci. 2006, 131:530-536.
11. Brunings A.M., Gabriel D.W. Xanthomonas citri: breaking the surface. Mol. Plant Pathol. 2003, 4:141-157.
12. Cardoso S.C., Barbosa-Mendes J.M., Boscariol-Camargo R.L., Christiano R.S.C., Filho A.B., Vieira M.L.C., Mendes B.M.J., Filho F.A.A.M. Transgenic sweet orange (Citrus sinensis L. Osbeck) expressing the attacin A gene for resistance to Xanthomonas citri subsp. citri. Plant Mol. Biol. Rep. 2009, Publish online.
13. Cervera M., José J., Navarro A., José A., Pina J.A., Navarro L., Peña L. Genetic transformation and regeneration of mature tissue of woody fruit plants bypassing the juvenile stage. Transgenic Res. 1998, 7:51-59.
14. Cervera M., José J., Navarro L., Peña L. Genetic transformation of mature citrus plants. Methods Mol. Biol. 2004, 286:177-187.
15. Chen S.C., Zhang J.R., Huang Z.R., Gao F., Chen F.Z., Long Y.Q. Studies on Agrobacterium-mediated antibacterial peptide D gene transfer in citrus. Sci. Agric. Sin. 1998, 3:7-13.
16. Civerolo E.L. Bacterial canker disease of citrus. J. Rio Grande Valle Hortic. Soc. 1984, 37:127-146.
17. Fagoaga C., Rodrigo I., Conejero V., Hinarejos C., Tuset J.J., Arnau J., Pina J.A., Navarro L., Peña L. Increased tolerance to Phytophthora citrophthora in transgenic orange plants constitutively expressing a tomato pathogenesis related protein PR-5. Mol. Breed. 2001, 7:175-185.
18. Fagoaga C., Lopez C.L., Mendoza H. Posttranscriptional gene silencing of the p23 silencing suppressor of Citrus tristeza virus confers resistance to the virus in transgenic Mexican lime. Plant Mol. Biol. 2006, 2:153-165.
19. Febres V.J., Lee R.F., Moore G.A. Transgenic resistance to Citrus tristeza virus in grapefruit. Plant Cell Rep. 2008, 27:93-104.
20. Ghorbel R., Domínguez A., Navarro L., Peña L. High efficiency genetic transformation of sour orange (Citrus aurantium L.) and production of transgenic trees containing the coat protein gene of Citrus tristeza virus. Tree Physiol. 2000, 20:1183-1189.
21. Gutiérrez M.A., Luth D.E., Moore G.A. Factors affecting Agrobacterium-mediated transformation in Citrus and production of sour orange (Citrus aurantium L.) plants expressing the coat protein gene of Citrus tristeza virus. Plant Cell Rep. 1997, 16:745-753.
22. Hu, R., Wei, H., Chen, S.C., He, Y.R., 2004. Construction of a vector with hepatitis A capsid protein gene and genetic transformation of Citrus sinensis Osbeck. Hereditas 4, 425-431.
23. Jaynes J.M., Nagpala P., Destefano-Beltran L., Huang J.H., Kim J.H., Denny T., Cetiner S. Expression of a Cecropin B lytic peptide analog in transgenic tobacco confers enhanced resistance to bacterial wilt caused by Pseudomonas solanacearum. Plant Sci. 1993, 89:43-53.
24. Jia S.R., Qu X.M., Feng L.X., Tang T., Tang Y.X. Development of potato clones with enhanced resistance to bacterial wilt by introducing antibacterial peptide gene. Sci. Agric. Sin. 1998, 3:5-12.
25. Kobayashi S., Uchimiya H. Expression and integration of a foreign gene in orange (Citrus sinensis Osb.) protonlasts by direct DNA transfer. Jpn. J. Genet. 1989, 64:91-97.
26. Kaneyoshi J., Kobayashi S., Nakamura Y., Shigemoto N., Doi Y. A simple and efficient gene transfer system of trifoliate orange (Poncirus trifoliata Raf.). Plant Cell Rep. 1994, 13:541-545.
27. Khawale R.N., Singh S.K., Garg G., Baranwal V.K., Ajirlo S.A. Agrobacterium-mediated genetic transformation of Nagpur mandarin (Citrus reticulate Blanco). Curr. Sci. 2006, 91:1700-1705.
28. Koizumi M., Kuhara S. Evaluation of citrus Plants for Resistance to Bacterial Canker Disease in Relation to the Lesion Extension 1982, Bull Fruit Tree Research Station, Japan D4, pp. 73-92.
29. Li M.J., Li W., Huang W.H., Jiang Z., Xia M.M., Qu X.M., Hu W.W., Jia S.R. Gene synthesis and cloning of Shiva A. Genetic Engineering of Chinese Potato Cultivars by Introducing Antibacterial Peptide Gene 1996, 114-118. Agriculture Technology Publishing, Beijing TX. S.R. Jia, X.M. Qu (Eds.).
30. Molinari H.B.C., Bespalhok J.C., Kobayashi A.K., Pereira L.F.P., Vieira L.G.P. Agrobacterium tumefaciens-mediated transformation of Swingle citrumelo (Citrus paradisi Macf X Poncirus trifoliata L. Raf.) using thin epicotyl sections. Sci. Hortic. 2004, 99:379-385.
31. Moore G.A., Jacono C.C., Neidigh J.L., Lawrence S.D., Cline K. Agrobacterium-mediated transformation of Citrus stem segments and regeneration of transgenic plants. Plant Cell Rep. 1992, 11:238-242.
32. Moore G.A., Febres V.J., Niblett C.L., McCaffery Luth D., Garnsey S.M. Agrobacterium-mediated transformation of grapefruit (Citrus paradisi macf.) with genes from Citrus tristeza virus. Acta Hortic. 2000, 535:237-243.
33. Omar A.A., Song W.Y., Grosser J.W. Introduction of Xa21, a Xanthomonas-resistance gene from rice, into Hamlin sweet orange (Citrus sinensis L. Osbeck) using protoplast-GFP co-transformation or single plasmid transformation. J. Hortic. Sci. Biotechnol. 2007, 82:914-923.
34. Peña L., Cervera M., Juárez J., Navarro A., Pina J.A., Durán-Vila N., Navarro L. Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants. Plant Cell Rep. 1995, 14:616-619.
35. Peña L., Cervera M., Juárez J., Navarro A., Pina J.A., Navarro L. Genetic transformation of lime (Citrus aurantifolia Swing.): factors affecting transformation and regeneration. Plant Cell Rep. 1997, 16:731-737.
36. Peña L., Martin-Trillo M., Juarez J., Pina J.A., Navarro L., Martinez-Zapater J.M. Constitutive expression of Arabidopsis LEAFY or APETALA1 genes in citrus reduces their generation time. Nat. Biotechnol. 2001, 19:263-267.
37. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual 2001, Cold Spring Harbor Laboratory Press, New York. 2nd ed.
38. Tan B., Li D.L., Xu S.X., Fan G.E., Fan J., Guo W.W. Highly efficient transformation of GFP and KNU genes into precocious trifoliate orange (Poncirus trifoliata [L.] Raf), a potential model genotype for functional genomics studies in citrus. Tree Genet. Genome 2009, 5:529-537.
39. Tang Q.Y., Feng M.G. DPS Data Processing System: Experimental Design, Statistical Analysis, and Data Mining 2007, Science Press, Beijing.
40. Van Hofsten P., Faye I., Kockum K., Lee J.Y., Xanthopoulos K.G., Boman I.A., Boman H.G., Engstromt A., Andreut D., Merrifieldt R.B. Molecular cloning, cDNA sequencing, and chemical synthesis of Cecropin B from Hyalophora cecropia. Proc. Natl. Acad. Sci. U.S.A. 1985, 82:2240-2243.
41. Wang Z.X., Jia S.R. Construction of vectors harboring a barley α-amylase signal sequence and a Cecropin B or Shiva A gene that resulted in the extracellular protein secretion in potato transgenic plants. J. Agric. Biotechnol. 1996, 3:277-286.
42. White F.F., Taylor B.H., Huffman G.A., Gordon M.P., Nester E.W. Molecular and genetic analysis of the transferred DNA regions of the root inducing plasmid of Agrobacterium rhizogenes. J. Bact. 1985, 164:33-34.
43. Xie Y., Min Y.J., Tang T., Feng L.X., Wang Q.S. Chemical synthesis and cloning of a Cecropin B gene. Chin. J. Biotechnol. 1990, 6:311-331.
44. Zanek M.C., Reyes C.A., Cervera M. Genetic transformation of sweet orange with the coat protein gene of Citrus psorosis virus and evaluation of resistance against the virus. Plant Cell Rep. 2008, 27:57-66.
45. Zasloff M. Antibiotic peptides as mediators of innate immunity. Curr. Opin. Immunol. 1992, 4:3-7.
46. Zhang Q.J., Zhang J.N., Huang Z.R., Tan S.C., Guo Z.Y. The bactericidal effect of antibacterial peptide from Chinese Oak Silkworm to the pathogen of bacterial ulcer and yellow shoot disease in citrus. Sci. Sericult. 1995, 2:77-81.