Genetic transformation of sweet orange with the coat protein gene of Citrus psorosis virus and evaluation of resistance against the virus

Plant Cell Reports

Volumn 27, Issue 1, 2008, Pages 57-66

  Zanek, María Cecilia ^a   Reyes, Carina Andrea ^a   Cervera, Magdalena ^c   Peña, Eduardo José ^a   Velázquez, Karelia ^a   Costa, Norma ^b   Plata, Maria Inés ^b   Grau, Oscar ^a   Peña, Leandro ^c   García, María Laura ^a  

^a Inst de Biotecnología Y Biología Molecular IBBM   (Argentina)

^b INSTITUTO NACIONAL DE TECNOLOGÍA AGROPECUARIA INTA   (Argentina)

^c INSTITUTO VALENCIANO DE INVESTIGACIONES AGRARIAS IVIA   (Spain)

Author keywords

Citrus; Citrus psorosis virus; PDR; Post transcriptional gene silencing; Transgenic trees

Indexed keywords

FORESTRY; GENE EXPRESSION; PATHOGENS; PROTEINS; RNA; VIRUSES;

CITRUS PSOROSIS VIRUS; GENETIC TRANSFORMATION; POST TRANSCRIPTIONAL GENE SILENCING; TRANSGENIC TREES;

FRUITS;

CAPSID PROTEIN;

ARTICLE; BIOLOGICAL MODEL; CITRUS FRUIT; ENZYME LINKED IMMUNOSORBENT ASSAY; GENETIC TRANSFORMATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; INNATE IMMUNITY; NORTHERN BLOTTING; PLANT DISEASE; PLANT VIRUS; PLASMID; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; TRANSGENIC PLANT; VIROLOGY;

BLOTTING, NORTHERN; BLOTTING, SOUTHERN; CAPSID PROTEINS; CITRUS; ENZYME-LINKED IMMUNOSORBENT ASSAY; IMMUNITY, NATURAL; MODELS, GENETIC; PLANT DISEASES; PLANT VIRUSES; PLANTS, GENETICALLY MODIFIED; PLASMIDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANSFORMATION, GENETIC;

FRUITS; NUCLEIC ACIDS; PATHOGENS; PROTEINS; TREES; VIRUSES;

CITRUS; CITRUS PSOROSIS; CITRUS PSOROSIS VIRUS; CITRUS SINENSIS; CITRUS X PARADISI; OPHIOVIRUS;

EID: 36949026682     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00299-007-0422-8     Document Type: Article

References (40)

1. Alioto D, Gangemi M, Deaglio S, Sposato S, Noris E, Luisoni E, Milne RG (1999) Improved detection of citrus psorosis virus using polyclonal and monoclonal antibodies. Plant Pathol 48:735-741. doi: 10.1046/j.1365-3059.1999. 00410.x
2. Barthe GA, Ceccardi TL, Manjunath KL, Derrick KS (1998) Citrus psorosis virus: nucleotide sequencing of the coat protein gene and detection by hybridisation and RT-PCR. J Gen Virol 79:1531-1537
3. Baulcombe DC (1996) Mechanisms of pathogen-derived resistance to viruses in transgenic plants. Plant Cell 8:1833-1844. doi: 10.1105/tpc.8.10.1833
4. Beachy RN, Loesch-Fries S, Tumer NE (1990) Coat protein-mediated resistance against virus infection. Annu Rev Phytopathol 28:451-474
5. Bekesiova I, Nap J-P, Mlynarova L (1999) Isolation of high Quality DNA and RNA from leaves of the carnivorous plant Drosera rotundifolia. Plant Mol Biol Rep 17:269-277
6. Beñatena HN, Portillo MM (1984) Natural spread of psorosis in sweet orange seedlings. In: Garnsey SM, Timmer LW and Dodds JA (eds) Proceedings of the 9th conference of the international organization of citrus virologists, IOCV, Riverside, pp 159-164
7. Cervera M (2005) Histochemical and fluorometric assays for uidA (GUS) gene detection. Methods Mol Biol 286:203-214
8. Danós E (1990) La psorosis de los cítricos: la epidemia en curso en Argentina y el desafio de su control. In: International Foundation for Science (IFS) e Instituto Nacional de Tecnologia Agropecuaria (INTA) (eds) Revista de Investigaciones Agropecuarias, vol 22, pp 265-277
9. Domínguez A, Guerri J, Cambra M, Navarro L, Moreno P, Peña L (2000) Efficient production of citrus transgenic plants expressing the coat protein gene of Citrus Tristeza Virus. Plant Cell Rep. 19:427-433. doi: 10.1007/s002990050751
10. Domínguez A, Hermoso de Mendoza A, Guerri J, Cambra M, Navarro L, Moreno P, Peña L (2002) Pathogen-derived resistance to Citrus tristeza virus (CTV) in transgenic Mexican lime (Citrus aurantifolia (Christ.) Swing.) plants expressing its p25 coat protein gene. Mol Breed 10:1-10. doi: 10.1023/A:1020347415333
11. Fagoaga C, López C, Hermoso de Mendoza A, Moreno P, Navarro L, Flores R, Peña L (2006) Post-transcriptional gene silencing of the p23 silencing supresor of Citrus tristeza virus confers resistance to the virus in transgenic Mexican lime. Plant Mol Biol 60:153-165. doi: 10.1007/s11103-005- 3129-7
12. Futterer J, Gisel A, Iglesias V, Kloti A, Kost B, Mittelsten Sceid O, Neuhaus-Url G, Schrott M, Shillito R, Spangenber G, Wang ZY (1995) Standard molecular techniques for analysis of trangenic plants. In: Potrykus I, Spangenberg G (eds) Gene tranfer to plants, Springer Lab manual, vol 25, pp 215
13. Gambino G, Gribaudo I, Stephan L, Scharti A, Laimer M (2005) Molecular characterization of grapevine plants transformed with GFLV resistance genes:I. Plant Cell Rep 24:665-662. doi: 10.1007/s00299-005-0006-4
14. García ML, Dal Bo E, Grau O, Milne R (1994) The closely related citrus ringspot and citrus psorosis viruses have particles of novel filamentous morphology. J Gen Virol 75:3585-3590
15. Garnsey SM, Timmer LW (1980) Mechanical transmissibility of citrus ringspot virus isolates from Florida, Texas, and California. In: Calavan EC, Garnsey SM, Timmer LW (eds) Proceedings of the Eighth conference of the international organization of citrus virologists, IOCV, Riverside, pp 174-179
16. Garnsey SM, Youtsey CO, Bridges GD, Burnett HC (1976) A necrotic ringspot-like virus found in a "Star Ruby" grapefruit tree imported without authorization from Texas. In: Proceedings of the Florida State Horticultural Society, vol 89, pp 63-67
17. Ghorbel R, Lopez C, Fagoaga C, Moreno P, Navarro L, Flores R, Peña L (2001) Transgenic citrus plants expressing the citrus tristeza virus p23 protein exhibit viral-like symptoms. Mol Plant Pathol 2:27-36
18. Goldbach R, Bucher, Prins M (2003) Resistance mechanisms to plant viruses: an overview. Virus Res 92:207-212. doi: 10.1016/S0168-1702(02)00353-2
19. Hily JH, Scorza R, Malinowski T, Zawadzka B, Ravelonandro M (2004) Stability of gene silencing-based resistance to Plum pox virus in transgenic plum (Prunus domestica L.) under field conditions. Transgenic Res 13:427-436. doi: 10.1007/s11248-004-8702-3
20. Kalantidis K, Psaradakis S, Tabler M, Tsagris M (2002) The occurrence of CMV-specific short RNAs in transgenic tobacco expressing virus-derived double-stranded RNA is indicative of resistence to the virus. Mol Plant Microbe Interact 15:826-833
21. Kouassi NK, Chen L, Siré C, Bangratz-Reyser M, Beachy RN, Fauquet CM, Brugidou C (2006) Expression of rice yellow mottle virus coat protein enhances virus infection in transgenic plants. Arch Virol 151:2111-2122. doi: 10.1007/s00705-006-0802-3
22. Levin JS, Thompson WF, Csinos AS, Stephenson MG, Weissinger AK (2005) Matrix attachment regions increase the efficiency and stability of RNA-mediated resistance to Tomato Spotted Wilt Virus in transgenic tobacco. Transgenic Res 14:193-206. doi: 10.1007/s11248-004-5413-8
23. Maghuly F, Stephan L, da Câmara Machado A, Borroto Fernandez E, Mahmood AK, Gambino G, Gribaudo I, Schartl A, Laimer M (2006) Molecular characterization of grapevine plants transformed with GFLV esistance genes: II. Plant Cell Rep 25:546-553. doi: 10.1007/s00299-005-0087-0
24. Milne RG, Garcia ML, Moreno P (2003) Citrus psorosis virus. Association of applied biologists (AAB) descriptions of plant viruses. http://www.dpvweb. net/dpv/showdpv.php?dpvno=401
25. Naum-Ongania G, Gago-Zachert S, Pena E, Grau O, Garcia ML (2003) Citrus psorosis virus RNA 1 is of negative polarity and potentially encodes in its complementary strand a 24 K protein of unknown function and 280 K putative RNA dependent RNA polymerase. Virus Res 96:49-61. doi: 10.1016/S0168-1702(03)00172-2
26. Palle SR, Miao H, Seyran M, Louzada ES, da Graça JV, Skaria M (2004) Preliminary evidence for natural transmission of citrus psorosis virus by an olpidium-like fungus. In: 16th conference of the international organization of citrus virologists, IOCV, Abstract conference, Mexico
27. Pang SZ, Nagpala P, Wang M, Slightom JL, Gonsalves D (1992) Resistance to heterologous isolates of tomato spotted wilt virus in transgenic tobacco expressing its nucleocapsid protein gene. Phytopathology 82:1223-1229
28. Peña L, Cervera M, Juárez J, Navarro A, Pina JA, Durán-Vila N, Navarro L (1995) Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plant. Plant Cell Rep 14:616-619. doi: 10.1007/BF00232724
29. Powell-Abel P, Nelson RS, De B, Hoffmann N, Rogers SG, Fraley RT, Beachy RN (1986) Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232:738-743. doi: 10.1126/science.3457472
30. Prins M de Haan P, Luyten R, van Veller M, van Grinsven MQ, Goldbach R (1995) Broad resistance to tospoviruses in transgenic tobacco plants expressing three tospoviral nucleoprotein gene sequences. Mol Plant Microbe Interact 8:85-91
31. Ravelonandro M, Scorza R, Bachelier JC, Labonne G, Levy L, Damsteegt V (1997) Resistance of Prunus domestica L. to plum pox virus infection. Plant Dis 81:1231-1235
32. Roistacher CN (1991) Graft-transmissible diseases of citrus. In: Food and Agriculture Organization of the United Nations, FAO (ed) Handbook for detection and diagnosis, Rome, pp 115-126
33. Roistacher CN (1993) Psorosis-A review. In: Moreno P, da Graça JV, Timmer LW (eds) Proceedings of the 12th conference of the international organization of citrus virologists, IOCV, Riverside, pp 139-154
34. Sánchez de la Torre ME, Riva O, Zandomeni R, Grau O, García ML (1998) Mol Plant Pathol. Online http://www.bspp.org.uk/mppol/1998/ 1019sanchez
35. Sánchez de la Torre ME, López C, Grau O, García ML (2002) RNA2 of citrus psorosis virus is of negative polarity and has a single open reading frame in its complementary strand. J Gen Virol 83:1777-1781
36. Sanford JC, Johnston SA (1985) The concept of parasite-derived resistance-deriving resistance genes from the parasite's own genome. J Theor Biol 113:395-405
37. Scorza R, Callahan A, Levy L, Damsteegt V, Webb K, Revelonandro M (2001) Post-transcriptional gene silencing in plum pox virus resistant transgenic European plum containing the plum potyvirus coat protein gene. Transgenic Res 10:201-209. doi: 10.1023/A:1016644823203
38. Swingle WT, Webber HJ (1896) The principal diseases of citrus fruits in Florida. United States Department of Agriculture, Division of Vegetable Physiology and Pathology, Bulletin 8
39. Vancanneyt G, Schmidt R, ÓConnor-Sanchez L, Willmitzer L, Rocha-Sosa M (1990) Construction of an intron-containing marker gene: splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation. Mol Gen Genet 220:245-250
40. Zanek MC, Peña EJ, Reyes CA, Figueroa J, Stein B, Grau O, García ML (2006) Detection of Citrus psorosis virus in the northwestern citrus production area of Argentina by using an improved TAS-ELISA. J Virol Methods 137:245-251. doi: 101016/jjviromet200606021