Effect of trenbolone acetate plus estradiol on transcriptional regulation of metabolism pathways in bovine liver

Hormone Molecular Biology and Clinical Investigation

Volumn 2, Issue 2, 2010, Pages 257-265

  Becker, Christiane ^a   Riedmaier, Irmgard ^a   Reiter, Martina ^b   Tichopad, Ales ^a   Pfaffl, Michael W ^a,b   Meyer, Heinrich H D ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b BioEPS GmbH   (Germany)

Author keywords

Anabolic steroids; Estradiol; Liver; mRNA gene expression; Principal components analysis; Reverse transcriptase quantitative polymerase chain reaction; Trenbolone acetate

Indexed keywords

17 BETA HYDROXY STEROID DEHYDROGENASE 2,3 HYDROXY METHYLGLUTARYL COENZYM A SYNTHASE; ANDROGEN RECEPTOR; APOLIPOPROTEIN; APOLIPOPROTEIN 2; CATHEPSIN B; ESTRADIOL; GLOBULIN; INSULIN RECEPTOR; INSULIN RECEPTOR ALPHA; INSULIN RECEPTOR BETA; SCATTER FACTOR; SOMATOMEDIN BINDING PROTEIN; SOMATOMEDIN C; STEROIDOGENIC ACUTE REGULATORY PROTEIN; SYNTHETASE; TRENBOLONE ACETATE; TUMOR NECROSIS FACTOR; TYROSINE AMINOTRANSFERASE; UNCLASSIFIED DRUG;

ADOLESCENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; FEMALE; GENE; HEIFER; LIVER METABOLISM; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RECEPTOR UPREGULATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 79951673320     PISSN: 18681883     EISSN: 18681891     Source Type: Journal    
DOI: 10.1515/HMBCI.2010.029     Document Type: Article

References (39)

1. Henricks DM, Brandt RT Jr, Titgemeyer EC, Milton CT. Serum concentrations of trenbolone-17 beta and estradiol-17 beta and performance of heifers treated with trenbolone acetate, melengestrol acetate, or estradiol-17 beta. J Anim Sci 1997;75: 2627-33.
2. Meyer HH. Biochemistry and physiology of anabolic hormones used for improvement of meat production. APMIS 2001;109: 1-8.
3. Urhausen A, Torsten A, Wilfried K. Reversibility of the effects on blood cells, lipids, liver function and hormones in former anabolic-androgenic steroid abusers. J Steroid Biochem Mol Biol 2003;84:369-75.
4. Goll DE, Thompson VF, Taylor RG, Christiansen JA. Role of the calpain system in muscle growth. Biochimie 1992;74: 225-37.
5. Flores EA, Bistrian BR, Pomposelli JJ, Dinarello CA, Blackburn GL, Istfan NW. Infusion of tumor necrosis factor/cachectin promotes muscle catabolism in the rat. A synergistic effect with interleukin 1. J Clin Invest 1989;83:1614-22.
6. Delhanty PJ. Interleukin-1 beta suppresses growth hormoneinduced acid-labile subunit mRNA levels and secretion in primary hepatocytes. Biochem Biophys Res Commun 1998;243: 269-72.
7. Saini A, Al-Shanti N, Stewart CE. Waste management-cytokines, growth factors and cachexia. Cytokine Growth Factor Rev 2006;17:475-86.
8. Lang CH, Frost RA, Vary TC. Skeletal muscle protein synthesis and degradation exhibit sexual dimorphism after chronic alcohol consumption but not acute intoxication. Am J Physiol Endocrinol Metab 2007;292:E1497-506.
9. Frost RA, Lang CH. Regulation of muscle growth by pathogenassociated molecules. J Anim Sci 2008;86:E84-93.
10. Labrie F, Luu-The V, Lin SX, Simard J, Labrie C. Role of 17 beta-hydroxysteroid dehydrogenases in sex steroid formation in peripheral intracrine tissues. Trends Endocrinol Metab 2000; 11:421-7.
11. Mindnich R, Moller G, Adamski J. The role of 17 betahydroxysteroid dehydrogenases. Mol Cell Endocrinol 2004; 218:7-20.
12. Viturro E, Koenning M, Kroemer A, Schlamberger G, Wiedemann S, Kaske M, Meyer HH. Cholesterol synthesis in the lactating cow: induced expression of candidate genes. J Steroid Biochem Mol Biol 2009;115:62-7.
13. Christenson LK, Strauss JF III. Steroidogenic acute regulatory protein (StAR) and the intramitochondrial translocation of cholesterol. Biochim Biophys Acta 2000;1529:175-87.
14. Ning Y, Xu L, Ren S, Pandak WM, Chen S, Yin L. StAR overexpression decreases serum and tissue lipids in apolipoprotein E-deficient mice. Lipids 2009;44:511-9.
15. Bonetti A, Tirelli F, Catapano A, Dazzi D, Dei CA, Solito F, Ceda G, Reverberi C, Monica C, Pipitone S, Elia G, Spattini M, Magnati G. Side effects of anabolic androgenic steroids abuse. Int J Sports Med 2008;29:679-87.
16. Kindermann W. Cardiovascular side effects of anabolic-androgenic steroids. Herz 2006;31:566-73.
17. Wu FC. Endocrine aspects of anabolic steroids. Clin Chem 1997;43:1289-92.
18. Giannitrapani L, Soresi M, La SE, Cervello M, D'Alessandro N, Montalto G. Sex hormones and risk of liver tumor. Ann NY Acad Sci 2006;1089:228-36.
19. d'Arville CN, Johnson PJ. Growth factors, endocrine aspects and hormonal treatment in hepatocellular carcinoma-an overview. J Steroid Biochem Mol Biol 1990;37:1007-12.
20. Verbeke R, Debackere M, Hicquet R, Lauwers H, Pottie G, Stevens J, Van Moer D, Van Hoof J, Vermeersch G. Quality of the meat after the application of anabolic agents in young calves. Environ Qual Saf Suppl 1976;5:123-30.
21. Pottier J, Busigny M, Grandadam JA. Plasma kinetics, excretion in mild and tissue levels in the cow following implantation of trenbolone acetate. J Anim Sci 1975;41:962-8.
22. Riedmaier I, Becker C, Pfaffl MW, Meyer HH. The use of omic technologies for biomarker development to trace functions of anabolic agents. J Chromatogr A 2009;1216:8192-9.
23. Prakash BS, Meyer HH, Schallenberger E, van de Wiel DF. Development of a sensitive enzyme immunoassay (EIA) for progesterone determination in unextracted bovine plasma using the second antibody technique. J Steroid Biochem 1987;28: 623-7.
24. Fleige S, Pfaffl MW. RNA integrity and the effect on the realtime qRT-PCR performance. Mol Aspects Med 2006;27:126-39.
25. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001;25:402-8.
26. Stahlberg A, Elbing K, Andrade-Garda JM, Sjogreen B, Forootan A, Kubista M. Multiway real-time PCR gene expression profiling in yeast Saccharomyces cerevisiae reveals altered transcriptional response of ADH-genes to glucose stimuli. BMC Genomics 2008;9:170.
27. Becker C, Hammerle-Fickinger A, Riedmaier I, Pfaffl MW. mRNA and microRNA quality control for RT-qPCR analysis. Methods 2010;50:237-43.
28. Pfaffl M, Schwarz F, Sauerwein H. Quantification of insulinlike growth factor-1 (IGF-1) mRNA: modulation of growth intensity by feeding results in inter-and intra-tissue-specific differences of IGF-1 mRNA expression in steers. Exp Clin Endocrinol Diabetes 1998;106:514-21.
29. Borski RJ, Tsai W, Mott-Friberg R, Barkan AL. Regulation of somatic growth and the somatotropic axis by gonadal steroids: primary effect on insulin-like growth factor I gene expression and secretion. Endocrinology 1996;137:3253-9.
30. White ME, Johnson BJ, Hathaway MR, Dayton WR. Growth factor messenger RNA levels in muscle and liver of steroidimplanted and nonimplanted steers. J Anim Sci 2003;81: 965-72.
31. Johnson BJ, Hathaway MR, Anderson PT, Meiske JC, Dayton WR. Stimulation of circulating insulin-like growth factor I (IGF-I) and insulin-like growth factor binding proteins (IGFBP) due to administration of a combined trenbolone acetate and estradiol implant in feedlot cattle. J Anim Sci 1996;74:372-9.
32. Johnson BJ, White ME, Hathaway MR, Christians CJ, Dayton WR. Effect of a combined trenbolone acetate and estradiol implant on steady-state IGF-I mRNA concentrations in the liver of wethers and the longissimus muscle of steers. J Anim Sci 1998;76:491-7.
33. Gockerman A, Prevette T, Jones JI, Clemmons DR. Insulin-like growth factor (IGF)-binding proteins inhibit the smooth muscle cell migration responses to IGF-I and IGF-II. Endocrinology 1995;136:4168-73.
34. Allen LB, Capps BE, Miller EC, Clemmons DR, Maile LA. Glucose-oxidized low-density lipoproteins enhance insulin-like growth factor I-stimulated smooth muscle cell proliferation by inhibiting integrin-associated protein cleavage. Endocrinology 2009;150:1321-9.
35. Glazer G. Atherogenic effects of anabolic steroids on serum lipid levels. A literature review. Arch Intern Med 1991;151: 1925-33.
36. Mooney MH, Bergwerff AA, van Meeuwen JA, Luppa PB, Elliott CT. Biosensor-based detection of reduced sex hormonebinding globulin binding capacities in response to growth-promoter administrations. Anal Chim Acta 2009;637:235-40.
37. Johnson RW, Roberson LE, Kenney FT. Regulation of tyrosine aminotransferase in rat liver. X. Characterization and interconversion of the multiple enzyme forms. J Biol Chem 1973; 248:4521-7.
38. Ing NH. Steroid hormones regulate gene expression posttranscriptionally by altering the stabilities of messenger RNAs. Biol Reprod 2005;72:1290-6.
39. Staton JM, Thomson AM, Leedman PJ. Hormonal regulation of mRNA stability and RNA-protein interactions in the pituitary. J Mol Endocrinol 2000;25:17-34.