Effects of a HP0859 (rfaD) knockout mutation on lipopolysaccharide structure of Helicobacter pylori 26695 and the bacterial adhesion on AGS cells

Biochemical and Biophysical Research Communications

Volumn 405, Issue 3, 2011, Pages 497-502

  Chang, Po Chun ^a   Wang, Chun Jen ^a   You, Chung Kai ^a   Kao, Mou Chieh ^a  

^a NATIONAL TSING HUA UNIVERSITY   (Taiwan)

Author keywords

Adhesion; ADP l glycero d manno heptose 6 epimerase; Helicobacter pylori; Lipopolysaccharide

Indexed keywords

BACTERIAL PROTEIN; HP0859 PROTEIN; LIPOPOLYSACCHARIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; NOVOBIOCIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GROWTH; BACTERIUM ADHERENCE; CIRCULAR DICHROISM; CONTROLLED STUDY; ESCHERICHIA COLI; GENE DISRUPTION; GENE INACTIVATION; GENETIC COMPLEMENTATION; HELICOBACTER PYLORI; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; PROTEIN STRUCTURE;

BACTERIAL ADHESION; CARBOHYDRATE EPIMERASES; CELL LINE, TUMOR; CLONING, MOLECULAR; GENE KNOCKOUT TECHNIQUES; HELICOBACTER PYLORI; HUMANS; LIPOPOLYSACCHARIDES; MUTATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HELICOBACTER PYLORI; HELICOBACTER PYLORI 26695;

EID: 79951570407     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2011.01.060     Document Type: Article

References (23)

1. Eaton K.A., Kersulyte D., Mefford M., Danon S.J., Krakowka S., Berg D.E. Role of Helicobacter pylori cag region genes in colonization and gastritis in two animal models. Infect. Immun. 2001, 69:2902-2908.
2. Raetz C.R., Whitfield C. Lipopolysaccharide endotoxins. Annu. Rev. Biochem. 2002, 71:635-700.
3. Aspinall G.O., Monteiro M.A., Pang H., Walsh E.J., Moran A.P. Lipopolysaccharide of the Helicobacter pylori type strain NCTC 11637 (ATCC 43504): structure of the O antigen chain and core oligosaccharide regions. Biochemistry 1996, 35:2489-2497.
4. Valvano M.A., Messner P., Kosma P. Novel pathways for biosynthesis of nucleotide-activated glycero-manno-heptose precursors of bacterial glycoproteins and cell surface polysaccharides. Microbiology 2002, 148:1979-1989.
5. Marais A., Mendz G.L., Hazell S.L., Megraud F. Metabolism and genetics of Helicobacter pylori: the genome era. Microbiol. Mol. Biol. Rev. 1999, 63:642-674.
6. Coleman W.G. The rfaD gene codes for ADP-l-glycero-d-mannoheptose-6-epimerase. An enzyme required for lipopolysaccharide core biosynthesis. J. Biol. Chem. 1983, 258:1985-1990.
7. Ding L., Seto B.L., Ahmed S.A., Coleman W.G. Purification and properties of the Escherichia coli K-12 NAD-dependent nucleotide diphosphosugar epimerase, ADP-l-glycero-d-mannoheptose 6-epimerase. J. Biol. Chem. 1994, 269:24384-24390.
8. Drazek E.S., Stein D.C., Deal C.D. A mutation in the Neisseria gonorrhoeae rfaD homolog results in altered lipooligosaccharide expression. J. Bacteriol. 1995, 177:2321-2327.
9. Coleman W.G., Leive L. Two mutations which affect the barrier function of the Escherichia coli K-12 outer membrane. J. Bacteriol. 1979, 139:899-910.
10. Haas R., Meyer T.F., van Putten J.P. Aflagellated mutants of Helicobacter pylori generated by genetic transformation of naturally competent strains using transposon shuttle mutagenesis. Mol. Microbiol. 1993, 8:753-760.
11. Croxen M.A., Ernst P.B., Hoffman P.S. Antisense RNA modulation of alkyl hydroperoxide reductase levels in Helicobacter pylori correlates with organic peroxide toxicity but not infectivity. J. Bacteriol. 2007, 189:3359-3368.
12. Ni Y., McPhie P., Deacon A., Ealick S., Coleman W.G. Evidence that NADP+ is the physiological cofactor of ADP-l-glycero-d-mannoheptose 6-epimerase. J. Biol. Chem. 2001, 276:27329-27334.
13. Gomi T., Takata Y., Fujioka M. Rat liver S-adenosylhomocysteinase. Spectrophotometric study of coenzyme binding. Biochim. Biophys. Acta 1989, 994:172-179.
14. Westphal O., Jann K. Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure. Methods Carbohydr. Chem. 1965, 5:83-91.
15. Tsai C.M., Frasch C.E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal. Biochem. 1982, 119:115-119.
16. Segal E.D., Cha J., Lo J., Falkow S., Tompkins L.S. Altered states: involvement of phosphorylated CagA in the induction of host cellular growth changes by Helicobacter pylori. Proc. Natl. Acad. Sci. USA 1999, 96:14559-14564.
17. Deacon A.M., Ni Y.S., Coleman W.G., Ealick S.E. The crystal structure of ADP-l-glycero-d-mannoheptose 6-epimerase: catalysis with a twist. Structure 2000, 8:453-462.
18. Walsh A.G., Matewish M.J., Burrows L.L., Monteiro M.A., Perry M.B., Lam J.S. Lipopolysaccharide core phosphates are required for viability and intrinsic drug resistance in Pseudomonas aeruginosa. Mol. Microbiol. 2000, 35:718-727.
19. Moran A.P. Molecular structure, biosynthesis, and pathogenic roles of lipopolysaccharides. Helicobacter pylori: Physiology and Genetics 2001, 81-95. American Society for Microbiology, Washington (DC). M. HLT, M. GL, H. SL (Eds.).
20. Edwards N.J., Monteiro M.A., Faller G., Walsh E.J., Moran A.P., Roberts I.S., High N.J. Lewis X structures in the O antigen side-chain promote adhesion of Helicobacter pylori to the gastric epithelium. Mol. Microbiol. 2000, 35:1530-1539.
21. Moran A.P., Sturegard E., Sjunnesson H., Wadstrom T., Hynes S.O. The relationship between O-chain expression and colonisation ability of Helicobacter pylori in a mouse model. FEMS Immunol. Med. Microbiol. 2000, 29:263-270.
22. Valkonen K.H., Wadstrom T., Moran A.P. Interaction of lipopolysaccharides of Helicobacter pylori with basement membrane protein laminin. Infect. Immun. 1994, 62:3640-3648.
23. Wirth H.P., Yang M., Karita M., Blaser M.J. Expression of the human cell surface glycoconjugates Lewis x and Lewis y by Helicobacter pylori isolates is related to cagA status. Infect. Immun. 1996, 64:4598-4605.