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Volumn 27, Issue 3, 2011, Pages 727-730

Reliable fusion PCR using Taq polymerases and pGEM-T easy vectors

Author keywords

Gene fusion; Overlap extension PCR; Primer design; Taq polymerase

Indexed keywords

CLONING; FUSION REACTIONS; GENES; POLYMERS;

EID: 79951555537     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-010-0498-0     Document Type: Article
Times cited : (1)

References (8)
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    • (1988) Nucleic Acids Res , vol.16 , pp. 9677-9686
    • Clark, J.M.1
  • 2
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    • Simultaneous introduction of multiple mutations using overlap extension PCR
    • Ge L, Rudolph P (1997) Simultaneous introduction of multiple mutations using overlap extension PCR. Biotechniques 22: 28-30.
    • (1997) Biotechniques , vol.22 , pp. 28-30
    • Ge, L.1    Rudolph, P.2
  • 3
    • 34248559599 scopus 로고    scopus 로고
    • Gene splicing and mutagenesis by PCR-driven overlap extension
    • Heckman KL, Pease LR (2007) Gene splicing and mutagenesis by PCR-driven overlap extension. Nat Protoc 2: 924-932.
    • (2007) Nat Protoc , vol.2 , pp. 924-932
    • Heckman, K.L.1    Pease, L.R.2
  • 4
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    • Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension
    • Horton RM, Hunt HD, Ho SN, Pullen JK, Pease LR (1989) Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77: 61-68.
    • (1989) Gene , vol.77 , pp. 61-68
    • Horton, R.M.1    Hunt, H.D.2    Ho, S.N.3    Pullen, J.K.4    Pease, L.R.5
  • 5
    • 41549105328 scopus 로고    scopus 로고
    • Use of the lambda red recombinase system to rapidly generate mutants in Pseudomonas aeruginosa
    • Lesic B, Rahme LG (2008) Use of the lambda red recombinase system to rapidly generate mutants in Pseudomonas aeruginosa. BMC Mol Biol 9: 20.
    • (2008) BMC Mol Biol , vol.9 , pp. 20
    • Lesic, B.1    Rahme, L.G.2
  • 8
    • 0031579372 scopus 로고    scopus 로고
    • Splicing by overlap extension by PCR using asymmetric amplification: an improved technique for the generation of hybrid proteins of immunological interest
    • Warrens AN, Jones MD, Lechler RI (1997) Splicing by overlap extension by PCR using asymmetric amplification: an improved technique for the generation of hybrid proteins of immunological interest. Gene 186: 29-35.
    • (1997) Gene , vol.186 , pp. 29-35
    • Warrens, A.N.1    Jones, M.D.2    Lechler, R.I.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.