Role of RhoA in platelet-derived growth factor-BB-induced migration of rat hepatic stellate cells

Chinese Medical Journal

Volumn 123, Issue 18, 2010, Pages 2502-2509

  Li, Lei ^a   Li, Jing ^a   Wang, Ji Yao ^a   Yang, Chang Qing ^b   Jia, Ming Lei ^a   Jiang, Wei ^a  

^a FUDAN UNIVERSITY   (China)

^b TONGJI UNIVERSITY   (China)

Author keywords

Cell movement; Hepatic stellate cells; Liver fibrosis; Platelet derived growth factor BB; Rhoa protein

Indexed keywords

BETA ACTIN; F ACTIN; GLUTATHIONE TRANSFERASE; MESSENGER RNA; PLATELET DERIVED GROWTH FACTOR BB; PROTEIN CDC42; RAC1 PROTEIN; RHO GUANINE NUCLEOTIDE BINDING PROTEIN; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; VINCULIN;

ANIMAL CELL; ARTICLE; CELL ACTIVATION; CELL MIGRATION; CELL MOTILITY; CELL STIMULATION; CONTROLLED STUDY; CYTOSKELETON; ENZYME ACTIVATION; ENZYME ASSAY; ENZYME INHIBITION; FOCAL ADHESION; GENETIC TRANSFECTION; INTRACELLULAR SIGNALING; MALE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN TARGETING; RAT; REGULATORY MECHANISM; SIGNAL TRANSDUCTION; STELLATE CELL; STRESS FIBER;

ANIMALS; BLOTTING, WESTERN; CELL LINE; CELL MOVEMENT; CELLS, CULTURED; FLUORESCENT ANTIBODY TECHNIQUE; GLUTATHIONE TRANSFERASE; HEPATIC STELLATE CELLS; MALE; PLATELET-DERIVED GROWTH FACTOR; POLYMERASE CHAIN REACTION; RATS; RATS, SPRAGUE-DAWLEY; RHOA GTP-BINDING PROTEIN;

EID: 79851480794     PISSN: 03666999     EISSN: None     Source Type: Journal    
DOI: 10.3760/cma.j.issn.0366-6999.2010.18.002     Document Type: Article

References (26)

1. Friedman SL. Mechanisms of hepatic fibrogenesis. Gastroenterology 2008; 134: 1655-1669.
2. Liu X, Zhu ST, You H, Cong M, Liu TH, Wang BE, et al. Hepatitis B virus infects hepatic stellate cells and affects their proliferation and expression of collagen type I. Chin Med J 2009; 122: 1455-1461.
3. Yang C, Zeisberg M, Mosterman B, Sudhakar A, Yerramalla U, Holthaus K, et al. Liver fibrosis: insights into migration of hepatic stellate cells in response to extracellular matrix and growth factors. Gastroenterology 2003; 124: 147-159.
4. Wang XM, Yu DM, McCaughan GW, Gorrell MD. Fibroblast activation protein increases apoptosis, cell adhesion, and migration by the LX-2 human stellate cell line. Hepatology 2005; 42: 935-945.
5. Dormann D, Weijer CJ. Imaging of cell migration. EMBO J 2006; 25: 3480-3493.
6. Le Clainche C, Carlier MF. Regulation of actin assembly associated with protrusion and adhesion in cell migration. Physiol Rev 2008; 88: 489-513.
7. Lanzetti L. Actin in membrane trafficking. Curr Opin Cell Biol 2007; 19: 453-458.
8. Lemonnier M, Landraud L, Lemichez E. Rho GTPase-activating bacterial toxins: from bacterial virulence regulation to eukaryotic cell biology. FEMS Microbiol Rev 2007; 31: 515-534.
9. Ridley AJ. Rho GTPases and cell migration. J Cell Sci 2001; 114: 2713-2722.
10. Moustakas A, Stournaras C. Regulation of actin organisation by TGF-beta in H-ras-transformed fibroblasts. J Cell Sci 1999; 112: 1169-1179.
11. Riccalton-Banks L, Bhandari R, Fry J, Shakesheff KM. A simple method for the simultaneous isolation of stellate cells and hepatocytes from rat liver tissue. Mol Cell Biochem 2003; 248: 97-102.
12. Moreira RK. Hepatic stellate cells and liver fibrosis. Arch Pathol Lab Med 2007; 131: 1728-1734.
13. Wynn TA. Cellular and molecular mechanisms of fibrosis. J Pathol 2008; 214: 199-210.
14. Andrae J, Gallini R, Betsholtz C. Role of platelet-derived growth factors in physiology and medicine. Genes Dev 2008; 22: 1276-1312.
15. Papakonstanti EA, Stournaras C. Cell responses regulated by early reorganization of actin cytoskeleton. FEBS Lett 2008; 582: 2120-2127.
16. Mierke CT. The role of vinculin in the regulation of the mechanical properties of cells. Cell Biochem Biophys 2009; 53: 115-126.
17. Le Clainche C, Carlier MF. Regulation of actin assembly associated with protrusion and adhesion in cell migration. Physiol Rev 2008; 88: 489-513.
18. Charest PG, Firtel RA. Big roles for small GTPases in the control of directed cell movement. Biochem J 2007; 401: 377-390.
19. Price LS, Collard JG. Regulation of the cytoskeleton by Rho-family GTPases: implications for tumour cell invasion. Semin Cancer Biol 2001; 11: 167-173.
20. O'Connor KL, Nguyen BK, Mercurio AM. RhoA function in lamellae formation and migration is regulated by the alpha6beta4 integrin and cAMP metabolism. J Cell Biol 2000; 148: 253-258.
21. Ray RM, Patel A, Viar MJ, McCormack SA, Zheng Y, Tigyi G, et al. RhoA inactivation inhibits cell migration but does not mediate the effects of polyamine depletion. Gastroenterology 2002; 123: 196-205.
22. Banyard J, Anand-Apte B, Symons M, Zetter BR. Motility and invasion are differentially modulated by Rho family GTPases. Oncogene 2000; 19: 580-591.
23. Allen WE, Zicha D, Ridley AJ, Jones GE. A role for Cdc42 in macrophage chemotaxis. J Cell Biol 1998; 141: 1147-1157.
24. Tominaga T, Sahai E, Chardin P, McCormick F, Courtneidge SA, Alberts AS. Diaphanous-related formins bridge Rho GTPase and Src tyrosine kinase signaling. Mol Cell 2000; 5: 13-25.
25. Kawada N, Seki S, Kuroki T, Kaneda K. ROCK inhibitor Y-27632 attenuates stellate cell contraction and portal pressure increase induced by endothelin-1. Biochem Biophys Res Commun 1999; 266: 296-300.
26. Fukushima M, Nakamuta M, Kohjima M, Kotoh K, Enjoji M, Kobayashi N, et al. Fasudil hydrochloride hydrate, a Rho-kinase (ROCK) inhibitor, suppresses collagen production and enhances collagenase activity in hepatic stellate cells. Liver Int 2005; 25: 829-838.